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The map label for this gene is cofH2 [H]

Identifier: 150399817

GI number: 150399817

Start: 1088186

End: 1089262

Strand: Reverse

Name: cofH2 [H]

Synonym: Mevan_1072

Alternate gene names: 150399817

Gene position: 1089262-1088186 (Counterclockwise)

Preceding gene: 150399819

Following gene: 150399816

Centisome position: 63.33

GC content: 31.57

Gene sequence:

>1077_bases
ATGGACTTAATTTCCTTTAAAGAAAAGGATATTTCAAAAAAAGAGTGCCTAGAATTATTTTTAGATACTGAAAACTTTTT
TGATATTTTAAAAGTTGCAGATGATATTAGAAAGGAATCTGTTGGGGATACGGTTACTTATGTGGTTAATACAAATATAG
AGACTACAAATGTCTGTATAATGGGTTGTAAATTCTGTGCTTTTGGAAAATCAAAAAATAGCTTGGAAGCATTTAAATTA
AATTCAGAAGAAATTGCAAAAAAAGCCCTTTTAGCAAAAAAAGCAGGATTTACGGAAGTTACAATTCATGGCGGAATTCA
TCCTGATGTAGATACTCATTTTCAAGTTGAAACACTAAAAAAAGTAAATGAAGTAACGAACCCGTTTGGTGGAATTTACG
TCCATGCATATTCACCACAGGAAATATTAAATGGTGCCGAAGTTGCTGGATTAAATGTAAAAGAAGCGTTAGAAATGCTT
TATGACGCAGGTTTAAGAACCATTCCTGGAACCGCTGCTGAAATTCTTAATGACAGCGTTAGAAATGAGATATGTCCTTT
AAAAATGCCCGTAAATAAATGGATTGAAATAATTAAGGAAGCCCATAAAATAGGATTTAAAACGACATCAACTATAATGT
ACGGGCATATTGAACAATACAGTCACGTTGTTGAGCATTTTTCCATTTTAAAGGAAATTCAAAAAGAAACTTCTGTATTT
ACAGAATTTATTCCGCTTTCATACTTATATGAAAATACGCCACTTTATAATTTGGGCAGGGTTAAAGACGGTGCAAACGG
GCTTTACGAATTAAAACTTTATGCAATTTCTAGAATATTTTTTAAAGATACGATTAAAAACATTCAAGTTCCAAGAGTAA
AACTTGGAACCAAATTAAGCCAAATTATTCTAAAATGTGGTGCAAACGACCTTGGTGGAACATTGATGGAAGATAAAGTT
TCAAAAGCTGCCGGAAGTACCTATGAAGATGCAAGTACTGAATTAATGGAAAATGCAATTAAGAATATAGGCAGAATTCC
GAAAAAAAGAACTACACTTTATGAAATAATTAAATAA

Upstream 100 bases:

>100_bases
TAAACAAATTATGGTAGGTATTAATTAAATTACTATACATGCCCAAATTTATATATAAAAAGGTATGAATTAATGAAACA
TTAATAAATTCGGTGATCTA

Downstream 100 bases:

>100_bases
TGGTATTATTATTAATATAATAATTATATAATGATATAATAACTAATAGAATAATAATTAAAATAAATATTTTTTTTAAA
TCCTAACACTCGGCAGGTGA

Product: FO synthase subunit 2

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 2 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVVNTNIETTNVCIMGCKFCAFGKSKNSLEAFKL
NSEEIAKKALLAKKAGFTEVTIHGGIHPDVDTHFQVETLKKVNEVTNPFGGIYVHAYSPQEILNGAEVAGLNVKEALEML
YDAGLRTIPGTAAEILNDSVRNEICPLKMPVNKWIEIIKEAHKIGFKTTSTIMYGHIEQYSHVVEHFSILKEIQKETSVF
TEFIPLSYLYENTPLYNLGRVKDGANGLYELKLYAISRIFFKDTIKNIQVPRVKLGTKLSQIILKCGANDLGGTLMEDKV
SKAAGSTYEDASTELMENAIKNIGRIPKKRTTLYEIIK

Sequences:

>Translated_358_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVVNTNIETTNVCIMGCKFCAFGKSKNSLEAFKL
NSEEIAKKALLAKKAGFTEVTIHGGIHPDVDTHFQVETLKKVNEVTNPFGGIYVHAYSPQEILNGAEVAGLNVKEALEML
YDAGLRTIPGTAAEILNDSVRNEICPLKMPVNKWIEIIKEAHKIGFKTTSTIMYGHIEQYSHVVEHFSILKEIQKETSVF
TEFIPLSYLYENTPLYNLGRVKDGANGLYELKLYAISRIFFKDTIKNIQVPRVKLGTKLSQIILKCGANDLGGTLMEDKV
SKAAGSTYEDASTELMENAIKNIGRIPKKRTTLYEIIK
>Mature_358_residues
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVVNTNIETTNVCIMGCKFCAFGKSKNSLEAFKL
NSEEIAKKALLAKKAGFTEVTIHGGIHPDVDTHFQVETLKKVNEVTNPFGGIYVHAYSPQEILNGAEVAGLNVKEALEML
YDAGLRTIPGTAAEILNDSVRNEICPLKMPVNKWIEIIKEAHKIGFKTTSTIMYGHIEQYSHVVEHFSILKEIQKETSVF
TEFIPLSYLYENTPLYNLGRVKDGANGLYELKLYAISRIFFKDTIKNIQVPRVKLGTKLSQIILKCGANDLGGTLMEDKV
SKAAGSTYEDASTELMENAIKNIGRIPKKRTTLYEIIK

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40123; Mature: 40123

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVVNTNIETTNVCI
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEE
MGCKFCAFGKSKNSLEAFKLNSEEIAKKALLAKKAGFTEVTIHGGIHPDVDTHFQVETLK
EECHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
KVNEVTNPFGGIYVHAYSPQEILNGAEVAGLNVKEALEMLYDAGLRTIPGTAAEILNDSV
HHHHHCCCCCCEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNEICPLKMPVNKWIEIIKEAHKIGFKTTSTIMYGHIEQYSHVVEHFSILKEIQKETSVF
HHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEFIPLSYLYENTPLYNLGRVKDGANGLYELKLYAISRIFFKDTIKNIQVPRVKLGTKLS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QIILKCGANDLGGTLMEDKVSKAAGSTYEDASTELMENAIKNIGRIPKKRTTLYEIIK
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MDLISFKEKDISKKECLELFLDTENFFDILKVADDIRKESVGDTVTYVVNTNIETTNVCI
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEE
MGCKFCAFGKSKNSLEAFKLNSEEIAKKALLAKKAGFTEVTIHGGIHPDVDTHFQVETLK
EECHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
KVNEVTNPFGGIYVHAYSPQEILNGAEVAGLNVKEALEMLYDAGLRTIPGTAAEILNDSV
HHHHHCCCCCCEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RNEICPLKMPVNKWIEIIKEAHKIGFKTTSTIMYGHIEQYSHVVEHFSILKEIQKETSVF
HHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEFIPLSYLYENTPLYNLGRVKDGANGLYELKLYAISRIFFKDTIKNIQVPRVKLGTKLS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QIILKCGANDLGGTLMEDKVSKAAGSTYEDASTELMENAIKNIGRIPKKRTTLYEIIK
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA