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The map label for this gene is cbiF [H]

Identifier: 150399710

GI number: 150399710

Start: 979453

End: 980217

Strand: Reverse

Name: cbiF [H]

Synonym: Mevan_0962

Alternate gene names: 150399710

Gene position: 980217-979453 (Counterclockwise)

Preceding gene: 150399711

Following gene: 150399707

Centisome position: 56.99

GC content: 32.29

Gene sequence:

>765_bases
ATGAAAAAAATGATTATTGTTGGAGCGGGCCCTGGAGATAAGGAATTACTTACAATAAAGGGCAAAAAAGCAATTGAAAA
TGCAGATATTTTAATATATGCAGGTTCCCTTGTAAATCCTGAAGTTTTAGAGTACAATATAAAAAATGCGAAAATCTATA
ACAGTGCAAAAATGACACTTGAAGAAGTAATATCTGTTGCTGTAGATGGAATTAACAATAATTTATCAGTTGTAAGGGTT
CATACTGGGGATCCGTCTTTATATGGTGCAATAAAAGAACAAATTGATGAATTAAAAAAGCATGATATAGATGTAGAAAT
AATTCCTGGAGTAACGTCTCTTTTTGCAGCCGCTGCAACATTAAAATCCGAATTAACGCTTCCAGATGTATCACAAACTG
TAATTATTACAAGACCTGAAGGGCGAACTAAAAAACCCGAAAAAGAAAGTCTAATCGCTCTTTCAAAACACAACGCTACA
ATGGCAATTTATCTTGGAACAGGAATGATAGAAAAAGTATGTGAAGAATTAATTGAAGGAGGATACAATAAAGATACAGC
AGTTTCGGTTGTATATCATGCATCATGGCCTGATGAAAAAAAAATAACTGGAACTTTAACTGATATTTCAAAAAAGGTTA
AGGAAGAAAAAATTACAAAAACGGCCCTAATCATAGTTGGAAATGCAATGAATCCGAAATTTTACGAATATTCAAAACTT
TATGACAAAGATTTTGAACACGAATATAGGGGTGTAAAACAGTAA

Upstream 100 bases:

>100_bases
TACGAAATTTTAAAAAAAGCAACTGAATAATTTATTAATTTTTATTTTAGTAATTTAAATGAATTTTAAATGAATTAATA
AATAATAACTCGGTGAAATT

Downstream 100 bases:

>100_bases
GATATTTAAAAATTAAATCAATTTTAATTCTTCTATTTTTTCCATTAAAATTTTTAAATCGGTTTTTTTTGAAATTTCTT
TTTCATCTGCTATTAATTTA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKKMIIVGAGPGDKELLTIKGKKAIENADILIYAGSLVNPEVLEYNIKNAKIYNSAKMTLEEVISVAVDGINNNLSVVRV
HTGDPSLYGAIKEQIDELKKHDIDVEIIPGVTSLFAAAATLKSELTLPDVSQTVIITRPEGRTKKPEKESLIALSKHNAT
MAIYLGTGMIEKVCEELIEGGYNKDTAVSVVYHASWPDEKKITGTLTDISKKVKEEKITKTALIIVGNAMNPKFYEYSKL
YDKDFEHEYRGVKQ

Sequences:

>Translated_254_residues
MKKMIIVGAGPGDKELLTIKGKKAIENADILIYAGSLVNPEVLEYNIKNAKIYNSAKMTLEEVISVAVDGINNNLSVVRV
HTGDPSLYGAIKEQIDELKKHDIDVEIIPGVTSLFAAAATLKSELTLPDVSQTVIITRPEGRTKKPEKESLIALSKHNAT
MAIYLGTGMIEKVCEELIEGGYNKDTAVSVVYHASWPDEKKITGTLTDISKKVKEEKITKTALIIVGNAMNPKFYEYSKL
YDKDFEHEYRGVKQ
>Mature_254_residues
MKKMIIVGAGPGDKELLTIKGKKAIENADILIYAGSLVNPEVLEYNIKNAKIYNSAKMTLEEVISVAVDGINNNLSVVRV
HTGDPSLYGAIKEQIDELKKHDIDVEIIPGVTSLFAAAATLKSELTLPDVSQTVIITRPEGRTKKPEKESLIALSKHNAT
MAIYLGTGMIEKVCEELIEGGYNKDTAVSVVYHASWPDEKKITGTLTDISKKVKEEKITKTALIIVGNAMNPKFYEYSKL
YDKDFEHEYRGVKQ

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=233, Percent_Identity=27.0386266094421, Blast_Score=88, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322922, Length=246, Percent_Identity=27.2357723577236, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28027; Mature: 28027

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMIIVGAGPGDKELLTIKGKKAIENADILIYAGSLVNPEVLEYNIKNAKIYNSAKMTL
CCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHEEEECCCCEEECHHHHHH
EEVISVAVDGINNNLSVVRVHTGDPSLYGAIKEQIDELKKHDIDVEIIPGVTSLFAAAAT
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
LKSELTLPDVSQTVIITRPEGRTKKPEKESLIALSKHNATMAIYLGTGMIEKVCEELIEG
HHHCCCCCCCCCEEEEECCCCCCCCCCHHHEEEEECCCCEEEEEECCCHHHHHHHHHHHC
GYNKDTAVSVVYHASWPDEKKITGTLTDISKKVKEEKITKTALIIVGNAMNPKFYEYSKL
CCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHH
YDKDFEHEYRGVKQ
HCCCHHHHHCCCCC
>Mature Secondary Structure
MKKMIIVGAGPGDKELLTIKGKKAIENADILIYAGSLVNPEVLEYNIKNAKIYNSAKMTL
CCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHEEEECCCCEEECHHHHHH
EEVISVAVDGINNNLSVVRVHTGDPSLYGAIKEQIDELKKHDIDVEIIPGVTSLFAAAAT
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
LKSELTLPDVSQTVIITRPEGRTKKPEKESLIALSKHNATMAIYLGTGMIEKVCEELIEG
HHHCCCCCCCCCEEEEECCCCCCCCCCHHHEEEEECCCCEEEEEECCCHHHHHHHHHHHC
GYNKDTAVSVVYHASWPDEKKITGTLTDISKKVKEEKITKTALIIVGNAMNPKFYEYSKL
CCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHH
YDKDFEHEYRGVKQ
HCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]