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The map label for this gene is pyrB

Identifier: 150399707

GI number: 150399707

Start: 977810

End: 978718

Strand: Reverse

Name: pyrB

Synonym: Mevan_0959

Alternate gene names: 150399707

Gene position: 978718-977810 (Counterclockwise)

Preceding gene: 150399710

Following gene: 150399706

Centisome position: 56.9

GC content: 33.66

Gene sequence:

>909_bases
ATGAGACATCTGATTTCAATGAGGGACATCGGGCGAGATGATATTCTTCGTATTTTGGAAGAATCTGAAAAAATGGAAGC
TGTTTTAAATGAAAAGGGGCATTCTGATATATTAAACGGCAAAATTTTAGCAACTCTTTTTTATGAGCCGTCTACAAGAA
CGAGATTGTCTTTCGAAACTGCAATGAAAAGGCTTGGTGGAAATGTTATTGGTTTTACAGATATTTCAAATACTTCGGTA
ACAAAAGGCGAGTCTTTAACTGATACTATAAAAGTAATAAGTGGATACAGCGATTTAATTGTTATCCGGCACCCTTCAGA
AGGTGCTGCAAGACTTTCAAGTGAGGTTTCAGGTGTTCCAGTAATAAATGCAGGCGATGGCTCAAACCAGCATCCAACAC
AAACACTTCTTGACTTATATACTATAAAGCGAGAAGTTGGTAAAATTGACGGCTTAAAAATTGCATTTATTGGCGACTTA
AAATATGGTAGAACTGTTCATTCATTATGCCAAGCGTTATCTCTGTTTAAAAATGTGGAACTACGCCTTATTTCTCCAGA
TGAATTAAAAATACCAAGGGAAGTTTTAGAATATATTGATGGAAAAGTTTTACTGAGTGAAACTAGTGAAATTAATATTG
AAGATGTTGATGTAGTTTACATGACAAGAATTCAAAAAGAGAGATTTATTGATTTAAACGAATATCAAAAAGTAAAAGGT
ACCTACAGGCTTTTAAAAGAGCACGTTTTGGAAAAAAATTTAATAATAATGCACCCGCTTCCAAGGGTAGATGAAATCGA
TTCAAAAGTCGATAGTTTAAATCAAGCAAAGTATTTCAAACAGTCTTTTTACGGAGTTCCGGTTAGAATGGCGATTTTAA
GCCTTTTATCAAAAGATTTGCAAAAATAA

Upstream 100 bases:

>100_bases
TATTTTGAACAAGTTCCATTAATTACACCTCGATATATTTATTTCTACAATAATTTTATATATATTTAATTTATAACTAA
TGAAAAATGCCGGTGGTTTA

Downstream 100 bases:

>100_bases
TCTGATTTTATATGTTTATTTTGGTAATTTATAACTTTTAAAAGCAGTTAATTTTTTATACTATTATGTCAATCACATAT
TATACTCGCATACTCTCTCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRHLISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFETAMKRLGGNVIGFTDISNTSV
TKGESLTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVPVINAGDGSNQHPTQTLLDLYTIKREVGKIDGLKIAFIGDL
KYGRTVHSLCQALSLFKNVELRLISPDELKIPREVLEYIDGKVLLSETSEINIEDVDVVYMTRIQKERFIDLNEYQKVKG
TYRLLKEHVLEKNLIIMHPLPRVDEIDSKVDSLNQAKYFKQSFYGVPVRMAILSLLSKDLQK

Sequences:

>Translated_302_residues
MRHLISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFETAMKRLGGNVIGFTDISNTSV
TKGESLTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVPVINAGDGSNQHPTQTLLDLYTIKREVGKIDGLKIAFIGDL
KYGRTVHSLCQALSLFKNVELRLISPDELKIPREVLEYIDGKVLLSETSEINIEDVDVVYMTRIQKERFIDLNEYQKVKG
TYRLLKEHVLEKNLIIMHPLPRVDEIDSKVDSLNQAKYFKQSFYGVPVRMAILSLLSKDLQK
>Mature_302_residues
MRHLISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFETAMKRLGGNVIGFTDISNTSV
TKGESLTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVPVINAGDGSNQHPTQTLLDLYTIKREVGKIDGLKIAFIGDL
KYGRTVHSLCQALSLFKNVELRLISPDELKIPREVLEYIDGKVLLSETSEINIEDVDVVYMTRIQKERFIDLNEYQKVKG
TYRLLKEHVLEKNLIIMHPLPRVDEIDSKVDSLNQAKYFKQSFYGVPVRMAILSLLSKDLQK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=299, Percent_Identity=41.8060200668896, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI38788445, Length=320, Percent_Identity=25.625, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=50.4885993485342, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786469, Length=229, Percent_Identity=27.9475982532751, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790703, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=318, Percent_Identity=47.4842767295598, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=302, Percent_Identity=42.3841059602649, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642586, Length=266, Percent_Identity=48.1203007518797, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_METVS (A6UQU0)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323474.1
- ProteinModelPortal:   A6UQU0
- SMR:   A6UQU0
- STRING:   A6UQU0
- GeneID:   5325899
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0959
- eggNOG:   arNOG04260
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34120; Mature: 34120

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFET
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEHHH
AMKRLGGNVIGFTDISNTSVTKGESLTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVP
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCE
VINAGDGSNQHPTQTLLDLYTIKREVGKIDGLKIAFIGDLKYGRTVHSLCQALSLFKNVE
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCE
LRLISPDELKIPREVLEYIDGKVLLSETSEINIEDVDVVYMTRIQKERFIDLNEYQKVKG
EEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
TYRLLKEHVLEKNLIIMHPLPRVDEIDSKVDSLNQAKYFKQSFYGVPVRMAILSLLSKDL
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QK
CC
>Mature Secondary Structure
MRHLISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFET
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEHHH
AMKRLGGNVIGFTDISNTSVTKGESLTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVP
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCE
VINAGDGSNQHPTQTLLDLYTIKREVGKIDGLKIAFIGDLKYGRTVHSLCQALSLFKNVE
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCE
LRLISPDELKIPREVLEYIDGKVLLSETSEINIEDVDVVYMTRIQKERFIDLNEYQKVKG
EEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
TYRLLKEHVLEKNLIIMHPLPRVDEIDSKVDSLNQAKYFKQSFYGVPVRMAILSLLSKDL
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA