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The map label for this gene is mptN [H]

Identifier: 150399662

GI number: 150399662

Start: 931533

End: 932348

Strand: Reverse

Name: mptN [H]

Synonym: Mevan_0912

Alternate gene names: 150399662

Gene position: 932348-931533 (Counterclockwise)

Preceding gene: 150399663

Following gene: 150399658

Centisome position: 54.2

GC content: 27.7

Gene sequence:

>816_bases
ATGAAAGTAGGAATTCTTGCGCATAAAAAAACTCCAGAAAACTTGATGATTTACGAGGAATTTTTAAAAAATAATGTTTC
AATCGAATTTATCGACCCACTTGAAATTATTAACGGGAATGAAAATTTTAACTTTGATCTGATATTATCTCGTGTAGAAA
GGGTTTTTTTAAATGAAGGGCTTTATGCATTAACCGAACTTCAAAATAATTTTAAAGTGATAAATTGTGCAGAAACAGTA
AATCTCTGTCAAAATAAATACCTTACGTATCAAAAATTAAAGGAAGTATCTCCTAAATCTATAATGACGTATTCAAAAGA
TTTTAATACGGTTTTTTCAAACGTAAAAAAATTATTTGGCTACCCATTTGTTTTAAAACCAATTTATGGGGGATATGGGG
AAGGGGTTTTGAAAATAAATTCTAAACATGAATTTTTAAATACTTTTAAAGAATTAACTAAAAATAACAGTGAAATTTTT
ATTCAAGAATATATCGAATATCTTCACGACATACGTGTTTTTGTAATAAATAACGAGATAATTGGGGCAATGGAAAGAAT
TCCAAAAAACAACTGGAAGGCAAATTATTCGCTTGGTGCAGAAATTAAAGAAATAGAACTTTCAAAAGATGTAAAAAATA
TGGTTTTAGACTCAGTAAAAAAAGTAGGTGCAGATATTGTAGGGGTAGACGTTTTAGTTTCAAAAACTAAAAATTATATT
TTGGAAATGAATATAACTCCGCAGTTTAGGGGAATGATGAATTTTGTAAATGTTTCAAGAAAAATAGTCGATTACTGTAT
GGACTTAATGGAATAA

Upstream 100 bases:

>100_bases
AAAATAATTTAAATGCGGGAACTGTAAAAATAAAACTTTCCTATAAAATGGGCGGGGATTTAGAATCAATAAAAGTTCAA
GTTTTAAAATAAGTGAAAAA

Downstream 100 bases:

>100_bases
AAAAATAAAAGACTTAAAAAATTAATTTTTTATTTATTCATTTGACTCATTTGAAAATACTTTTCTAACCTCCTCTAAAG
CAGGTGTTTTTGCAAGAATG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKVGILAHKKTPENLMIYEEFLKNNVSIEFIDPLEIINGNENFNFDLILSRVERVFLNEGLYALTELQNNFKVINCAETV
NLCQNKYLTYQKLKEVSPKSIMTYSKDFNTVFSNVKKLFGYPFVLKPIYGGYGEGVLKINSKHEFLNTFKELTKNNSEIF
IQEYIEYLHDIRVFVINNEIIGAMERIPKNNWKANYSLGAEIKEIELSKDVKNMVLDSVKKVGADIVGVDVLVSKTKNYI
LEMNITPQFRGMMNFVNVSRKIVDYCMDLME

Sequences:

>Translated_271_residues
MKVGILAHKKTPENLMIYEEFLKNNVSIEFIDPLEIINGNENFNFDLILSRVERVFLNEGLYALTELQNNFKVINCAETV
NLCQNKYLTYQKLKEVSPKSIMTYSKDFNTVFSNVKKLFGYPFVLKPIYGGYGEGVLKINSKHEFLNTFKELTKNNSEIF
IQEYIEYLHDIRVFVINNEIIGAMERIPKNNWKANYSLGAEIKEIELSKDVKNMVLDSVKKVGADIVGVDVLVSKTKNYI
LEMNITPQFRGMMNFVNVSRKIVDYCMDLME
>Mature_271_residues
MKVGILAHKKTPENLMIYEEFLKNNVSIEFIDPLEIINGNENFNFDLILSRVERVFLNEGLYALTELQNNFKVINCAETV
NLCQNKYLTYQKLKEVSPKSIMTYSKDFNTVFSNVKKLFGYPFVLKPIYGGYGEGVLKINSKHEFLNTFKELTKNNSEIF
IQEYIEYLHDIRVFVINNEIIGAMERIPKNNWKANYSLGAEIKEIELSKDVKNMVLDSVKKVGADIVGVDVLVSKTKNYI
LEMNITPQFRGMMNFVNVSRKIVDYCMDLME

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=288, Percent_Identity=21.5277777777778, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31410; Mature: 31410

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGILAHKKTPENLMIYEEFLKNNVSIEFIDPLEIINGNENFNFDLILSRVERVFLNEG
CCCEEEECCCCCCHHHHHHHHHHCCCCEEEECHHHEECCCCCCCHHHHHHHHHHHHHHCC
LYALTELQNNFKVINCAETVNLCQNKYLTYQKLKEVSPKSIMTYSKDFNTVFSNVKKLFG
HHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
YPFVLKPIYGGYGEGVLKINSKHEFLNTFKELTKNNSEIFIQEYIEYLHDIRVFVINNEI
CCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCEEEEECCHH
IGAMERIPKNNWKANYSLGAEIKEIELSKDVKNMVLDSVKKVGADIVGVDVLVSKTKNYI
HHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
LEMNITPQFRGMMNFVNVSRKIVDYCMDLME
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGILAHKKTPENLMIYEEFLKNNVSIEFIDPLEIINGNENFNFDLILSRVERVFLNEG
CCCEEEECCCCCCHHHHHHHHHHCCCCEEEECHHHEECCCCCCCHHHHHHHHHHHHHHCC
LYALTELQNNFKVINCAETVNLCQNKYLTYQKLKEVSPKSIMTYSKDFNTVFSNVKKLFG
HHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
YPFVLKPIYGGYGEGVLKINSKHEFLNTFKELTKNNSEIFIQEYIEYLHDIRVFVINNEI
CCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCEEEEECCHH
IGAMERIPKNNWKANYSLGAEIKEIELSKDVKNMVLDSVKKVGADIVGVDVLVSKTKNYI
HHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEE
LEMNITPQFRGMMNFVNVSRKIVDYCMDLME
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]