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The map label for this gene is selB [H]

Identifier: 150399398

GI number: 150399398

Start: 697073

End: 698479

Strand: Reverse

Name: selB [H]

Synonym: Mevan_0647

Alternate gene names: 150399398

Gene position: 698479-697073 (Counterclockwise)

Preceding gene: 150399401

Following gene: 150399397

Centisome position: 40.61

GC content: 32.13

Gene sequence:

>1407_bases
ATGGATTTTATAAATATTAATTTGGGTATTTTTGGACATATTGATCATGGAAAGACAACACTTTCAAAAGTTTTAACTGA
AATTGCATCAACATCAGCACTTGATAAGCTTCCGGAATCTCAAAAACGTGGAATTACTATAGATATTGGATTTTCTTCTT
TTAAGCTTGAAAATTATCGTGTAACTCTTGTAGATTCTCCAGGACATGCAGATTTGATTAGGGCAGTAGTTAGTGCTGCA
GATATAATTGACCTAGCATTAATTGTTGTAGATGCAAAAGAGGGCCCAAAAACGCAAACTGGGGAACACATGCTTATTTT
AGACCACTTCAACATTCCAACAATAGTGGTAATGACAAAGGTTGATAATGCAAATCCTGATGAAATTAATAGGACAAAGC
TGTTTATGCAAACCATACTTAAGTCAACTAAAAATCTTAAAGAAAGTTTAATAATCCCAATTTCTGCAAAGACTGATTTT
GGAATTTCTGAACTTAAAAACACGATTTTTAAAACGTTAAATGATATGGAAATAGTTAGAAAAACAGATAGCTATTTTAA
AATGCCAATAGACCATGCATTTCCTATAAAAGGTGCAGGAACCGTTGTAACGGGGACTATAATTAAGGGAACTGTTAAAA
TAGGGGATGAATTGAGGATAATTCCAATAAATATCGGTGCAAAAGTAAGGAGTATACAATGTTTTAAAGAAAGTGTAACT
TTTGCAAAAGCAGGTGATCGAGTTGGGATGGCAATTCAAGGGGTGGACTCAAAACAAATCTATCGAGGATGTATATTAAC
TTCAAAAGATACTAAATTACAGGCAGTTAATAAAATTGTTGCTAAAATAAGGGTTTCAGATATATTTAAATACAATTTAA
CGCCTAAAATGAAGGTTCACTTAAATGTGGGAATGTTAATTGTTCCAGCAGTAATTGTGCCTTTTAAAAAGGTAGATTTT
AGAAATTATACTGAAAATGTTATTTTAGATGAAGTAGTTTCTGGAAATGAATGCTATTGTGCGTTTGAACTTGAAGAAAA
AGTTTTAGCAGAATTTGGCGATAGGGTTTTAATTACGAGGCTTGACCTTCCACCAACAACACTAAGAATCTGCGGTCATG
GCGTAATTGAAGAATTTAAACCTATAAAAGACTTAAATATTAAAAAGGAAGTTTTAAAAGAAGGAAATATTAAAGTAGAC
AAGGGAAGAACCGTAATTGAAGGACTTTCCGAATCAAAAGTCGCTGCTGAAAAATTAATTGGTGAAGAGATTTCAATTGT
TGGAAAAGATATTGTTGGGAAAATAAAAAGCACTTTTGGAACAAAAGGACTTTTAATGGCGGACTTTAATGGAAATGTTG
AAAATCGTGATAAAATAAATTTAAAACGACTTAGAAGGTGGGGTTAG

Upstream 100 bases:

>100_bases
AATGACTTTTACTTTGTTTAATCCTTTTTATACTTAATATAATATATAGTCTAGGGAGCTAAGAATACATAAACTTTAGA
ATAAAAAAGGGTAGAATGAC

Downstream 100 bases:

>100_bases
GTAAAAACATGACATTAACTGTTAAAACACCTTCAAAAGTCATATTATTTGGGGAACATGCGGTTGTAGATGGATATTCT
GCAATTGCAATGGCAATAGA

Product: selenocysteine-specific translation elongation factor

Products: GDP; phosphate

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MDFININLGIFGHIDHGKTTLSKVLTEIASTSALDKLPESQKRGITIDIGFSSFKLENYRVTLVDSPGHADLIRAVVSAA
DIIDLALIVVDAKEGPKTQTGEHMLILDHFNIPTIVVMTKVDNANPDEINRTKLFMQTILKSTKNLKESLIIPISAKTDF
GISELKNTIFKTLNDMEIVRKTDSYFKMPIDHAFPIKGAGTVVTGTIIKGTVKIGDELRIIPINIGAKVRSIQCFKESVT
FAKAGDRVGMAIQGVDSKQIYRGCILTSKDTKLQAVNKIVAKIRVSDIFKYNLTPKMKVHLNVGMLIVPAVIVPFKKVDF
RNYTENVILDEVVSGNECYCAFELEEKVLAEFGDRVLITRLDLPPTTLRICGHGVIEEFKPIKDLNIKKEVLKEGNIKVD
KGRTVIEGLSESKVAAEKLIGEEISIVGKDIVGKIKSTFGTKGLLMADFNGNVENRDKINLKRLRRWG

Sequences:

>Translated_468_residues
MDFININLGIFGHIDHGKTTLSKVLTEIASTSALDKLPESQKRGITIDIGFSSFKLENYRVTLVDSPGHADLIRAVVSAA
DIIDLALIVVDAKEGPKTQTGEHMLILDHFNIPTIVVMTKVDNANPDEINRTKLFMQTILKSTKNLKESLIIPISAKTDF
GISELKNTIFKTLNDMEIVRKTDSYFKMPIDHAFPIKGAGTVVTGTIIKGTVKIGDELRIIPINIGAKVRSIQCFKESVT
FAKAGDRVGMAIQGVDSKQIYRGCILTSKDTKLQAVNKIVAKIRVSDIFKYNLTPKMKVHLNVGMLIVPAVIVPFKKVDF
RNYTENVILDEVVSGNECYCAFELEEKVLAEFGDRVLITRLDLPPTTLRICGHGVIEEFKPIKDLNIKKEVLKEGNIKVD
KGRTVIEGLSESKVAAEKLIGEEISIVGKDIVGKIKSTFGTKGLLMADFNGNVENRDKINLKRLRRWG
>Mature_468_residues
MDFININLGIFGHIDHGKTTLSKVLTEIASTSALDKLPESQKRGITIDIGFSSFKLENYRVTLVDSPGHADLIRAVVSAA
DIIDLALIVVDAKEGPKTQTGEHMLILDHFNIPTIVVMTKVDNANPDEINRTKLFMQTILKSTKNLKESLIIPISAKTDF
GISELKNTIFKTLNDMEIVRKTDSYFKMPIDHAFPIKGAGTVVTGTIIKGTVKIGDELRIIPINIGAKVRSIQCFKESVT
FAKAGDRVGMAIQGVDSKQIYRGCILTSKDTKLQAVNKIVAKIRVSDIFKYNLTPKMKVHLNVGMLIVPAVIVPFKKVDF
RNYTENVILDEVVSGNECYCAFELEEKVLAEFGDRVLITRLDLPPTTLRICGHGVIEEFKPIKDLNIKKEVLKEGNIKVD
KGRTVIEGLSESKVAAEKLIGEEISIVGKDIVGKIKSTFGTKGLLMADFNGNVENRDKINLKRLRRWG

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI54607086, Length=366, Percent_Identity=33.6065573770492, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI34147630, Length=306, Percent_Identity=26.797385620915, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI223555963, Length=356, Percent_Identity=27.8089887640449, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI5729864, Length=356, Percent_Identity=27.8089887640449, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI4503471, Length=333, Percent_Identity=25.5255255255255, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4503475, Length=335, Percent_Identity=26.2686567164179, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI157426893, Length=226, Percent_Identity=26.5486725663717, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI25306283, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI19923640, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI25306287, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI46094014, Length=311, Percent_Identity=23.7942122186495, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729339, Length=229, Percent_Identity=27.5109170305677, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI53729337, Length=229, Percent_Identity=27.5109170305677, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=37.4045801526718, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI194018522, Length=326, Percent_Identity=21.4723926380368, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018520, Length=326, Percent_Identity=21.4723926380368, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI194097354, Length=326, Percent_Identity=21.4723926380368, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367247, Length=276, Percent_Identity=31.8840579710145, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789737, Length=287, Percent_Identity=30.6620209059233, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790412, Length=287, Percent_Identity=30.6620209059233, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789108, Length=279, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789559, Length=209, Percent_Identity=32.0574162679426, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=36.7647058823529, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788922, Length=138, Percent_Identity=29.7101449275362, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17506081, Length=410, Percent_Identity=29.5121951219512, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17506079, Length=406, Percent_Identity=28.0788177339901, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556456, Length=316, Percent_Identity=29.4303797468354, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25141371, Length=279, Percent_Identity=28.673835125448, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17552884, Length=322, Percent_Identity=27.0186335403727, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17569207, Length=322, Percent_Identity=27.0186335403727, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=219, Percent_Identity=26.9406392694064, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI115532067, Length=311, Percent_Identity=24.7588424437299, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115532065, Length=311, Percent_Identity=24.7588424437299, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=212, Percent_Identity=26.8867924528302, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566301, Length=160, Percent_Identity=28.125, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=326, Percent_Identity=30.3680981595092, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325337, Length=316, Percent_Identity=26.8987341772152, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319594, Length=316, Percent_Identity=26.8987341772152, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=23.2558139534884, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320863, Length=267, Percent_Identity=24.7191011235955, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=173, Percent_Identity=31.7919075144509, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322937, Length=377, Percent_Identity=23.342175066313, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922690, Length=507, Percent_Identity=29.9802761341223, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI281363316, Length=306, Percent_Identity=30.0653594771242, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17864358, Length=306, Percent_Identity=30.0653594771242, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19921738, Length=292, Percent_Identity=30.4794520547945, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652838, Length=322, Percent_Identity=27.9503105590062, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17137572, Length=322, Percent_Identity=27.9503105590062, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI45553807, Length=341, Percent_Identity=26.6862170087977, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45553816, Length=341, Percent_Identity=26.6862170087977, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24651721, Length=341, Percent_Identity=26.6862170087977, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17864154, Length=341, Percent_Identity=26.6862170087977, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550900, Length=389, Percent_Identity=25.1928020565553, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=24.4444444444444, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI78706572, Length=246, Percent_Identity=25.609756097561, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922512, Length=342, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24654852, Length=342, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28572034, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 51832; Mature: 51832

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFININLGIFGHIDHGKTTLSKVLTEIASTSALDKLPESQKRGITIDIGFSSFKLENYR
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCEEEEECEE
VTLVDSPGHADLIRAVVSAADIIDLALIVVDAKEGPKTQTGEHMLILDHFNIPTIVVMTK
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEE
VDNANPDEINRTKLFMQTILKSTKNLKESLIIPISAKTDFGISELKNTIFKTLNDMEIVR
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHH
KTDSYFKMPIDHAFPIKGAGTVVTGTIIKGTVKIGDELRIIPINIGAKVRSIQCFKESVT
CCCCCEECCCCCCCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHH
FAKAGDRVGMAIQGVDSKQIYRGCILTSKDTKLQAVNKIVAKIRVSDIFKYNLTPKMKVH
HHHCCCCCCEEEECCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEE
LNVGMLIVPAVIVPFKKVDFRNYTENVILDEVVSGNECYCAFELEEKVLAEFGDRVLITR
EECCHHHHHHHHHCCHHCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHCCCEEEEEE
LDLPPTTLRICGHGVIEEFKPIKDLNIKKEVLKEGNIKVDKGRTVIEGLSESKVAAEKLI
ECCCCHHHHHHCCHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHC
GEEISIVGKDIVGKIKSTFGTKGLLMADFNGNVENRDKINLKRLRRWG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MDFININLGIFGHIDHGKTTLSKVLTEIASTSALDKLPESQKRGITIDIGFSSFKLENYR
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCEEEEECEE
VTLVDSPGHADLIRAVVSAADIIDLALIVVDAKEGPKTQTGEHMLILDHFNIPTIVVMTK
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEE
VDNANPDEINRTKLFMQTILKSTKNLKESLIIPISAKTDFGISELKNTIFKTLNDMEIVR
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHH
KTDSYFKMPIDHAFPIKGAGTVVTGTIIKGTVKIGDELRIIPINIGAKVRSIQCFKESVT
CCCCCEECCCCCCCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHH
FAKAGDRVGMAIQGVDSKQIYRGCILTSKDTKLQAVNKIVAKIRVSDIFKYNLTPKMKVH
HHHCCCCCCEEEECCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEE
LNVGMLIVPAVIVPFKKVDFRNYTENVILDEVVSGNECYCAFELEEKVLAEFGDRVLITR
EECCHHHHHHHHHCCHHCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHCCCEEEEEE
LDLPPTTLRICGHGVIEEFKPIKDLNIKKEVLKEGNIKVDKGRTVIEGLSESKVAAEKLI
ECCCCHHHHHHCCHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHC
GEEISIVGKDIVGKIKSTFGTKGLLMADFNGNVENRDKINLKRLRRWG
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10860743 [H]