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The map label for this gene is sdhA [C]

Identifier: 150399339

GI number: 150399339

Start: 640937

End: 642577

Strand: Reverse

Name: sdhA [C]

Synonym: Mevan_0588

Alternate gene names: 150399339

Gene position: 642577-640937 (Counterclockwise)

Preceding gene: 150399340

Following gene: 150399338

Centisome position: 37.36

GC content: 34.49

Gene sequence:

>1641_bases
ATGATAACAGATATACTTATTATTGGTGGAGGAGGTGCTGCAGCAAGGGCAGCAATAGAGTGCGGACAGAAAAATGTAAT
TATCGTATCAAAAGGTCTTTTTGGAAAAAGCGGCTGTACAGTCATGGCAGAAGGGGGCTATAACGCAGTATTTAATCCAA
AAGATGGGTTTGAATCACACTTTAAAGACACGATGAAGGGTGGCGGATTTATAAATAATCCAAAGCTTGTAGAAATTTTG
GTAAATGATGCCCCATCTGAATTTAAAAACTTGGAAAATTTTGGATGCTTATTTGATAGAACCAGTGAAGCAGAATTTTC
ACAAAGGCCATTTGGAGGTCAAAGCTTTAATCGGACATGTTATAGCGGGGATAGGACTGGCCATGAAATGATATTAGGAC
TTATGGAATACTTAAGCAAATTTGATAGGATAAAAATAATGGAAGATGTAATGGCGGTAAAGCTAATTACTGATGAAATC
AAAACAAACGGAAAAAAAATTAAAAAGTGTTTTGGCGCTGTTTTTTTAAATCTATTAACTGGCCATATCTTTCCAATTTA
TGCAAAATCTACAATTCTTGCAACAGGGGGTGCAGGACAAATTTACCCGATTACTTCAAACCCAAAACAAAAAGTTGGAG
ATGGATTTGCAATTGCGTATAGGGAAGGCGTTGAATTAATTGACATGGAAATGGTTCAATTTCACCCGACAGGAATGCTT
GGAACAGGAATTTTAGTAACTGAAGCAGTACGTGGGGAAGGTGGGATACTATACAATAAAAATAAAGAAAGATTTATGAA
AAATTACGACCCCCAAAAATTAGAGCTTTCTACAAGAGATGTAGTTTCTAAAGCAATATTTAATGAAATTATGGATGGAA
GAGGAGTTGACGGCGGAGTATATCTTGATGTAACGCACCTAAATCCAGAAATAATTAAAGAAAAGCTTGAAACAATGTAT
TCTCAGTTTAGGGCCATTGGAATAGATATAACAAAAGAACCGATGAAAGTATCACCAACTGCACACCACTTCATGGGCGG
AATTAAAATAAATGAACAGTGTGAAACGAATATTTCTGGGTTATATGCTTGCGGTGAAGTATGTGGTGGAATTCACGGTG
CAAACAGGCTTGGCGGAAATGCACTTGCAGATACCCAAGTTTTTGGCGCAATTTCTGGGAAAAACGCTTTAAAAAATTCA
GAAAAAAATGAATTTGATATTTCGAACGTCTACCAAAAAACATGCGAATTTATCAATAGTTTAGAATCATGTTTAAAATC
TGAAAAAGAGGATTCAAACTCCGGAACTAACGTTTACGTTCTCATCGATGAACTGAGAAATTTAATGTGGGATCATGTTT
CAATTTCAAGAGACGAAACTGGGCTTAAAATTGCATTAAAAAATATAAATGAAATAAAAGAAAAAATGAAAAAAGTTTCA
ATAAATGGAATAGTTGATTTTTCAAAAAAATTGGAACTTGAAAACATGTTATTAGTTTCTGAAGTTGTAACGAAATGTGC
ACTTTTAAGAAAAGAAACAAGGGGGGCACATACAAGGACTGATTATCCGGAAACAAACGAGGAATGTGTTGGAAATTTTG
TAGCAGATTTGAATGGAATTAAATTTGTAAAAATAAAATAA

Upstream 100 bases:

>100_bases
TGGAATTGGTCTCTGAAAATGGAATTATATCCTTACAGGAAATACACATATTTAAAAATCTAAAATGATTTTGAAACGAT
AATTTAATTTTGGTGGTTCT

Downstream 100 bases:

>100_bases
TTTAAATTTTTTTTAATTTGCAGGAATGATTACTGTATCTAAACTTAATGACTCATAATTAAAAAAGATTATTAATTTAT
TGTTTTCGTCCATACGTGTT

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MITDILIIGGGGAAARAAIECGQKNVIIVSKGLFGKSGCTVMAEGGYNAVFNPKDGFESHFKDTMKGGGFINNPKLVEIL
VNDAPSEFKNLENFGCLFDRTSEAEFSQRPFGGQSFNRTCYSGDRTGHEMILGLMEYLSKFDRIKIMEDVMAVKLITDEI
KTNGKKIKKCFGAVFLNLLTGHIFPIYAKSTILATGGAGQIYPITSNPKQKVGDGFAIAYREGVELIDMEMVQFHPTGML
GTGILVTEAVRGEGGILYNKNKERFMKNYDPQKLELSTRDVVSKAIFNEIMDGRGVDGGVYLDVTHLNPEIIKEKLETMY
SQFRAIGIDITKEPMKVSPTAHHFMGGIKINEQCETNISGLYACGEVCGGIHGANRLGGNALADTQVFGAISGKNALKNS
EKNEFDISNVYQKTCEFINSLESCLKSEKEDSNSGTNVYVLIDELRNLMWDHVSISRDETGLKIALKNINEIKEKMKKVS
INGIVDFSKKLELENMLLVSEVVTKCALLRKETRGAHTRTDYPETNEECVGNFVADLNGIKFVKIK

Sequences:

>Translated_546_residues
MITDILIIGGGGAAARAAIECGQKNVIIVSKGLFGKSGCTVMAEGGYNAVFNPKDGFESHFKDTMKGGGFINNPKLVEIL
VNDAPSEFKNLENFGCLFDRTSEAEFSQRPFGGQSFNRTCYSGDRTGHEMILGLMEYLSKFDRIKIMEDVMAVKLITDEI
KTNGKKIKKCFGAVFLNLLTGHIFPIYAKSTILATGGAGQIYPITSNPKQKVGDGFAIAYREGVELIDMEMVQFHPTGML
GTGILVTEAVRGEGGILYNKNKERFMKNYDPQKLELSTRDVVSKAIFNEIMDGRGVDGGVYLDVTHLNPEIIKEKLETMY
SQFRAIGIDITKEPMKVSPTAHHFMGGIKINEQCETNISGLYACGEVCGGIHGANRLGGNALADTQVFGAISGKNALKNS
EKNEFDISNVYQKTCEFINSLESCLKSEKEDSNSGTNVYVLIDELRNLMWDHVSISRDETGLKIALKNINEIKEKMKKVS
INGIVDFSKKLELENMLLVSEVVTKCALLRKETRGAHTRTDYPETNEECVGNFVADLNGIKFVKIK
>Mature_546_residues
MITDILIIGGGGAAARAAIECGQKNVIIVSKGLFGKSGCTVMAEGGYNAVFNPKDGFESHFKDTMKGGGFINNPKLVEIL
VNDAPSEFKNLENFGCLFDRTSEAEFSQRPFGGQSFNRTCYSGDRTGHEMILGLMEYLSKFDRIKIMEDVMAVKLITDEI
KTNGKKIKKCFGAVFLNLLTGHIFPIYAKSTILATGGAGQIYPITSNPKQKVGDGFAIAYREGVELIDMEMVQFHPTGML
GTGILVTEAVRGEGGILYNKNKERFMKNYDPQKLELSTRDVVSKAIFNEIMDGRGVDGGVYLDVTHLNPEIIKEKLETMY
SQFRAIGIDITKEPMKVSPTAHHFMGGIKINEQCETNISGLYACGEVCGGIHGANRLGGNALADTQVFGAISGKNALKNS
EKNEFDISNVYQKTCEFINSLESCLKSEKEDSNSGTNVYVLIDELRNLMWDHVSISRDETGLKIALKNINEIKEKMKKVS
INGIVDFSKKLELENMLLVSEVVTKCALLRKETRGAHTRTDYPETNEECVGNFVADLNGIKFVKIK

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=531, Percent_Identity=35.0282485875706, Blast_Score=275, Evalue=7e-74,
Organism=Escherichia coli, GI1790597, Length=526, Percent_Identity=34.4106463878327, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1788928, Length=531, Percent_Identity=33.7099811676083, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1786942, Length=538, Percent_Identity=33.457249070632, Blast_Score=267, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17505833, Length=544, Percent_Identity=35.6617647058824, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17550100, Length=542, Percent_Identity=34.5018450184502, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI71986328, Length=445, Percent_Identity=27.6404494382022, Blast_Score=105, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=544, Percent_Identity=35.1102941176471, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322416, Length=544, Percent_Identity=34.7426470588235, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6322511, Length=423, Percent_Identity=29.3144208037825, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320788, Length=482, Percent_Identity=27.8008298755187, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24663005, Length=545, Percent_Identity=33.394495412844, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI17137288, Length=539, Percent_Identity=33.5807050092764, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24655642, Length=539, Percent_Identity=33.5807050092764, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24655647, Length=539, Percent_Identity=33.5807050092764, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 60122; Mature: 60122

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDILIIGGGGAAARAAIECGQKNVIIVSKGLFGKSGCTVMAEGGYNAVFNPKDGFESH
CCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCEEECCCCCHHHH
FKDTMKGGGFINNPKLVEILVNDAPSEFKNLENFGCLFDRTSEAEFSQRPFGGQSFNRTC
HHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCC
YSGDRTGHEMILGLMEYLSKFDRIKIMEDVMAVKLITDEIKTNGKKIKKCFGAVFLNLLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GHIFPIYAKSTILATGGAGQIYPITSNPKQKVGDGFAIAYREGVELIDMEMVQFHPTGML
CCEEEEEECCEEEEECCCCEEEECCCCCHHHHCCCEEEEECCCCCEEEEHHHEECCCCCC
GTGILVTEAVRGEGGILYNKNKERFMKNYDPQKLELSTRDVVSKAIFNEIMDGRGVDGGV
CCCHHEEHHHCCCCCEEECCCHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
YLDVTHLNPEIIKEKLETMYSQFRAIGIDITKEPMKVSPTAHHFMGGIKINEQCETNISG
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCEECCHHCCCCHH
LYACGEVCGGIHGANRLGGNALADTQVFGAISGKNALKNSEKNEFDISNVYQKTCEFINS
HHHHHHHHCCCCCCHHCCCCCCCCHHHHEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHH
LESCLKSEKEDSNSGTNVYVLIDELRNLMWDHVSISRDETGLKIALKNINEIKEKMKKVS
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
INGIVDFSKKLELENMLLVSEVVTKCALLRKETRGAHTRTDYPETNEECVGNFVADLNGI
HHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCE
KFVKIK
EEEEEC
>Mature Secondary Structure
MITDILIIGGGGAAARAAIECGQKNVIIVSKGLFGKSGCTVMAEGGYNAVFNPKDGFESH
CCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCEEECCCCCHHHH
FKDTMKGGGFINNPKLVEILVNDAPSEFKNLENFGCLFDRTSEAEFSQRPFGGQSFNRTC
HHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCC
YSGDRTGHEMILGLMEYLSKFDRIKIMEDVMAVKLITDEIKTNGKKIKKCFGAVFLNLLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GHIFPIYAKSTILATGGAGQIYPITSNPKQKVGDGFAIAYREGVELIDMEMVQFHPTGML
CCEEEEEECCEEEEECCCCEEEECCCCCHHHHCCCEEEEECCCCCEEEEHHHEECCCCCC
GTGILVTEAVRGEGGILYNKNKERFMKNYDPQKLELSTRDVVSKAIFNEIMDGRGVDGGV
CCCHHEEHHHCCCCCEEECCCHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
YLDVTHLNPEIIKEKLETMYSQFRAIGIDITKEPMKVSPTAHHFMGGIKINEQCETNISG
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCEECCHHCCCCHH
LYACGEVCGGIHGANRLGGNALADTQVFGAISGKNALKNSEKNEFDISNVYQKTCEFINS
HHHHHHHHCCCCCCHHCCCCCCCCHHHHEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHH
LESCLKSEKEDSNSGTNVYVLIDELRNLMWDHVSISRDETGLKIALKNINEIKEKMKKVS
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
INGIVDFSKKLELENMLLVSEVVTKCALLRKETRGAHTRTDYPETNEECVGNFVADLNGI
HHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCE
KFVKIK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]