Click here to switch to the map view.

The map label for this gene is cobQ

Identifier: 150399282

GI number: 150399282

Start: 582211

End: 583686

Strand: Reverse

Name: cobQ

Synonym: Mevan_0530

Alternate gene names: 150399282

Gene position: 583686-582211 (Counterclockwise)

Preceding gene: 150399283

Following gene: 150399281

Centisome position: 33.93

GC content: 29.47

Gene sequence:

>1476_bases
ATGGCTAAGTTTATAATGGTTGTTGGAACGGCTTCAAACAGTGGAAAAACAGTTTTAGTTTCGGGAATTTGTAGGATGCT
TGTAAATCAAGGATACAAGGTAGCTCCTTTTAAATCCCAAAACATGAGTTTAAATTCAAGGGTAAGTGTCGAAGATGGGG
AAATTGCAGTTGCACAGTACACTCAAGCTATTGCTGCAAAGGTTAATCCTTCAATTCACTTTAATCCCGTTCTTTTAAAG
CCAAAGGGAAATTTTATTTCGCAAGTTATAGTTCACGGAAAGCCGTACAAAGACATGGATTACAACGAATATCGAAAAGA
AAAGAACTATTTTCTTGAAAAAATAAAAGAAAGTATTGAATATCTTGATAAAAATTACGATTACGTTGTAATTGAGGGTG
CTGGAAGTTGTTGTGAAATAAATCTTTTAAATGATGATATTGCAAATTTAAAAGTTGCAGAACTTTCAAATGCAGATGCA
ATTTTAGTTTCAGATATCGATAGAGGAGGAGTTTTTGCTTCAATTTACGGGACTGTTAATTTACTTCCAGAAAATTGGAA
AAAATTACTGAAAGGTTTTGTAATTAATAAATTCAGAGGAAATATTGATGTACTAAAAGACGGGTTTGAAAAAATTGAAG
AACTTACAAATATTCCGGTAATTGGTACGATTCCTTATGATGAAACATTAGTTTTACCTGAAGAAGATAGCCAGGCAATA
CAGGGAAAAAAAGTTTTTGGAAACTTAAAAAGCCCAGTGGAAGTAAATATAGTTAAATTTTCAAAAATTGCAAATTTTAC
TGACTTAGACCCTCTTTCAAATGACTGTTTAATGAAATATATTGACTTTAATGATGATATTACTGGAGATATCTTAATTC
TTCCTGGAACTCGATGTTCAACTGTTGAAATGAATTTAATGAAAAAATATGGGTTGGATAAAAAAATCCACGAATTTATA
AATAAAGGAGGCATAATTTTTGGAATTTGTGGAGGATATCAAACTTTAGGAAAAATTTTAATTGATGAAAATTTTTCTGA
AGGAAATGTTGGAACAATTTCTGGACTAGATGTATTTAATATGGAAACAACTTTTGGAAATAAAAAGGCGATAAATAACT
CTAATGGAATTTTAAAAATAAATGATATGGAATATGAAGTTTCAGGTTATGAATTACATGAAGGATATAGTGTTTCAAAA
GAAACTCCGTTAATAACACTTTCAAATGGTTTTGGAAATCTTGGGGACATGTATGACGGCTCCTTTAAACAAATTGAAAA
TTCATATATTTTTGGAACGTACCTTCATGGAATTTTAGAAAATTTTGAGTTTAGGAACTATCTTGTAAATTTGGTGCTTT
CCAAAAAGAATTTAAAAAACATAAGTAAAGATAATTATGAAAAAGTACTTCAGGAAAACATGGATAAACTTTCAAAAATA
ATTGAGGAAAGTATTGACTTTTCAAAAATATTATAA

Upstream 100 bases:

>100_bases
TTTTAATATTTTTCAGTAACTATATATGTTCGATTTTTCTATATTCGTTTTAAAGAAAAGTTTAAGTGATATCATTATAA
TAAAATTTTCTGGTGAATAA

Downstream 100 bases:

>100_bases
AATTTAAACTAGCTTTTTTTAATAACTTTTTTTAATAAACAAATTTTTTTAATAATTAAGTACATATTTTTTTAAGGGTT
ATTTTGGAGGGGTAAAAAAA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAKFIMVVGTASNSGKTVLVSGICRMLVNQGYKVAPFKSQNMSLNSRVSVEDGEIAVAQYTQAIAAKVNPSIHFNPVLLK
PKGNFISQVIVHGKPYKDMDYNEYRKEKNYFLEKIKESIEYLDKNYDYVVIEGAGSCCEINLLNDDIANLKVAELSNADA
ILVSDIDRGGVFASIYGTVNLLPENWKKLLKGFVINKFRGNIDVLKDGFEKIEELTNIPVIGTIPYDETLVLPEEDSQAI
QGKKVFGNLKSPVEVNIVKFSKIANFTDLDPLSNDCLMKYIDFNDDITGDILILPGTRCSTVEMNLMKKYGLDKKIHEFI
NKGGIIFGICGGYQTLGKILIDENFSEGNVGTISGLDVFNMETTFGNKKAINNSNGILKINDMEYEVSGYELHEGYSVSK
ETPLITLSNGFGNLGDMYDGSFKQIENSYIFGTYLHGILENFEFRNYLVNLVLSKKNLKNISKDNYEKVLQENMDKLSKI
IEESIDFSKIL

Sequences:

>Translated_491_residues
MAKFIMVVGTASNSGKTVLVSGICRMLVNQGYKVAPFKSQNMSLNSRVSVEDGEIAVAQYTQAIAAKVNPSIHFNPVLLK
PKGNFISQVIVHGKPYKDMDYNEYRKEKNYFLEKIKESIEYLDKNYDYVVIEGAGSCCEINLLNDDIANLKVAELSNADA
ILVSDIDRGGVFASIYGTVNLLPENWKKLLKGFVINKFRGNIDVLKDGFEKIEELTNIPVIGTIPYDETLVLPEEDSQAI
QGKKVFGNLKSPVEVNIVKFSKIANFTDLDPLSNDCLMKYIDFNDDITGDILILPGTRCSTVEMNLMKKYGLDKKIHEFI
NKGGIIFGICGGYQTLGKILIDENFSEGNVGTISGLDVFNMETTFGNKKAINNSNGILKINDMEYEVSGYELHEGYSVSK
ETPLITLSNGFGNLGDMYDGSFKQIENSYIFGTYLHGILENFEFRNYLVNLVLSKKNLKNISKDNYEKVLQENMDKLSKI
IEESIDFSKIL
>Mature_490_residues
AKFIMVVGTASNSGKTVLVSGICRMLVNQGYKVAPFKSQNMSLNSRVSVEDGEIAVAQYTQAIAAKVNPSIHFNPVLLKP
KGNFISQVIVHGKPYKDMDYNEYRKEKNYFLEKIKESIEYLDKNYDYVVIEGAGSCCEINLLNDDIANLKVAELSNADAI
LVSDIDRGGVFASIYGTVNLLPENWKKLLKGFVINKFRGNIDVLKDGFEKIEELTNIPVIGTIPYDETLVLPEEDSQAIQ
GKKVFGNLKSPVEVNIVKFSKIANFTDLDPLSNDCLMKYIDFNDDITGDILILPGTRCSTVEMNLMKKYGLDKKIHEFIN
KGGIIFGICGGYQTLGKILIDENFSEGNVGTISGLDVFNMETTFGNKKAINNSNGILKINDMEYEVSGYELHEGYSVSKE
TPLITLSNGFGNLGDMYDGSFKQIENSYIFGTYLHGILENFEFRNYLVNLVLSKKNLKNISKDNYEKVLQENMDKLSKII
EESIDFSKIL

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_METVS (A6UPL5)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323049.1
- ProteinModelPortal:   A6UPL5
- STRING:   A6UPL5
- GeneID:   5325841
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0530
- eggNOG:   arNOG04142
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54909; Mature: 54777

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 330-330 ACT_SITE 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIMVVGTASNSGKTVLVSGICRMLVNQGYKVAPFKSQNMSLNSRVSVEDGEIAVAQY
CCEEEEEEEECCCCCCEEHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCEEHHHH
TQAIAAKVNPSIHFNPVLLKPKGNFISQVIVHGKPYKDMDYNEYRKEKNYFLEKIKESIE
HHHHHHHCCCCEEECEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLDKNYDYVVIEGAGSCCEINLLNDDIANLKVAELSNADAILVSDIDRGGVFASIYGTVN
HHHCCCCEEEEECCCCEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEE
LLPENWKKLLKGFVINKFRGNIDVLKDGFEKIEELTNIPVIGTIPYDETLVLPEEDSQAI
ECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHH
QGKKVFGNLKSPVEVNIVKFSKIANFTDLDPLSNDCLMKYIDFNDDITGDILILPGTRCS
CCHHHHCCCCCCEEEEEEEEEHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCC
TVEMNLMKKYGLDKKIHEFINKGGIIFGICGGYQTLGKILIDENFSEGNVGTISGLDVFN
EEHHHHHHHHCCCHHHHHHHHCCCEEEEECCCHHHHHHHEEECCCCCCCCEEEECCEEEE
METTFGNKKAINNSNGILKINDMEYEVSGYELHEGYSVSKETPLITLSNGFGNLGDMYDG
EEECCCCCCCCCCCCCEEEEECCEEEECCEEEECCCCCCCCCCEEEEECCCCCCCCCCCC
SFKQIENSYIFGTYLHGILENFEFRNYLVNLVLSKKNLKNISKDNYEKVLQENMDKLSKI
CHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
IEESIDFSKIL
HHHCCCHHHCC
>Mature Secondary Structure 
AKFIMVVGTASNSGKTVLVSGICRMLVNQGYKVAPFKSQNMSLNSRVSVEDGEIAVAQY
CEEEEEEEECCCCCCEEHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCEEHHHH
TQAIAAKVNPSIHFNPVLLKPKGNFISQVIVHGKPYKDMDYNEYRKEKNYFLEKIKESIE
HHHHHHHCCCCEEECEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLDKNYDYVVIEGAGSCCEINLLNDDIANLKVAELSNADAILVSDIDRGGVFASIYGTVN
HHHCCCCEEEEECCCCEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEE
LLPENWKKLLKGFVINKFRGNIDVLKDGFEKIEELTNIPVIGTIPYDETLVLPEEDSQAI
ECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHH
QGKKVFGNLKSPVEVNIVKFSKIANFTDLDPLSNDCLMKYIDFNDDITGDILILPGTRCS
CCHHHHCCCCCCEEEEEEEEEHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCC
TVEMNLMKKYGLDKKIHEFINKGGIIFGICGGYQTLGKILIDENFSEGNVGTISGLDVFN
EEHHHHHHHHCCCHHHHHHHHCCCEEEEECCCHHHHHHHEEECCCCCCCCEEEECCEEEE
METTFGNKKAINNSNGILKINDMEYEVSGYELHEGYSVSKETPLITLSNGFGNLGDMYDG
EEECCCCCCCCCCCCCEEEEECCEEEECCEEEECCCCCCCCCCEEEEECCCCCCCCCCCC
SFKQIENSYIFGTYLHGILENFEFRNYLVNLVLSKKNLKNISKDNYEKVLQENMDKLSKI
CHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
IEESIDFSKIL
HHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA