Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 150399082

GI number: 150399082

Start: 377181

End: 378365

Strand: Reverse

Name: trpB [H]

Synonym: Mevan_0328

Alternate gene names: 150399082

Gene position: 378365-377181 (Counterclockwise)

Preceding gene: 150399083

Following gene: 150399081

Centisome position: 22.0

GC content: 40.68

Gene sequence:

>1185_bases
ATGAAGTGTGAAGGATACTTTGGAGAATTTGGTGGGCAGTTTATCCCTGAAGTTTTGATGCCTGCAATTGATGAACTAAA
AGAAGCGTATTTTGAATTAAAAGACGATGAAGATTTTCAAAATGAGCTTTCTTACTATTTGAAACATTATGCCGGGCGTG
AAACTCCGCTTTACTACGCTGAAAACCTGACTAAAAAGCTTGGAGGTGCAAAAATCTACTTAAAACGGGAAGATTTGCTT
CACGGCGGGGCTCACAAAACGAATAACACTATTGGTCAGGCATTACTTGCTAAAAGAATGGGCAAAAAAAGAATAATTGC
AGAAACTGGTGCCGGGCAGCACGGTGTTGGAACTTCGATGGCAGGAGCACTATTTGGACTTGAAACAGAAATTTTTATGG
GGCGCCTTGATACTGAAAGACAGCAACCAAACGTATTTAGAATGAAACTTCTCGGTGCAAAGGTAACTCCTGTAGATACT
GGGTCAAAAGTATTAAAAGACGCTGTAAACGAAGCAATGAGAAACTGGACTGCAACTTTTGAAAACACGCACTATTTAAT
CGGAACTGTTATGGGGCCACATCCTTTCCCGACTATTGTAAGGGATTTCCAGTCCGTAATAGGAAAAGAGGTTAAAAAAC
AAATGACGGAACAGGAAGGAAGGTTTCCCGACTATTTAGTTGCATGCATTGGTGGCGGAAGTAATGCACTTGGTTTATTC
CACGCATTTATTCCAGATAAGGAAGTTAAGATGATCGGAATTGAAGCAGCTGGAAAAGGCCTTGATACAAGCTTACACGG
TGCATCTATTTCAAAAGGAAAAAAAGGAGTTCTTCACGGAATGCTTTCGTACTTTTTACAGGATGAAGATGGTCAAATCG
AAGAAGCATACAGTATTTCGGCAGGACTAGATTACCCCGGAATTGGCCCTGAACATGCATATTTACACAATTCTGGGCGA
GCAGAATACGCATCAGCAACTGATGAACAGGCATTAAATGCACTTATGGAACTTACAAGAAGTGAGGGAATAATTCCAGC
TCTGGAATCATCACATGCAGTAGCTTATGCAATTGAAAATGCGGGAACCATGGATAAGGATGATATAATGGTCATAAACC
TTTCAGGGCGTGGCGACAAGGATTTAAACACGGTAATTGACATAATGCAAAAAAGAGGGATGTAA

Upstream 100 bases:

>100_bases
AGCCGTTTGGAGTTGACGTTTCAAGCGGAGTTGAAAATAAAAATTCAAAGGACGAAGGATTAATAAAAAAATTTTATGAA
AATGTAAAATCGGTGAAATT

Downstream 100 bases:

>100_bases
ATGAATAAACCTGTATTAGTTAGTTTTTTAGTTTCCGGGGACCCAAATGCAGACGCAACGTTAAAATTCATGAAAGCACT
TGATAAATATTCTGGCGTAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKCEGYFGEFGGQFIPEVLMPAIDELKEAYFELKDDEDFQNELSYYLKHYAGRETPLYYAENLTKKLGGAKIYLKREDLL
HGGAHKTNNTIGQALLAKRMGKKRIIAETGAGQHGVGTSMAGALFGLETEIFMGRLDTERQQPNVFRMKLLGAKVTPVDT
GSKVLKDAVNEAMRNWTATFENTHYLIGTVMGPHPFPTIVRDFQSVIGKEVKKQMTEQEGRFPDYLVACIGGGSNALGLF
HAFIPDKEVKMIGIEAAGKGLDTSLHGASISKGKKGVLHGMLSYFLQDEDGQIEEAYSISAGLDYPGIGPEHAYLHNSGR
AEYASATDEQALNALMELTRSEGIIPALESSHAVAYAIENAGTMDKDDIMVINLSGRGDKDLNTVIDIMQKRGM

Sequences:

>Translated_394_residues
MKCEGYFGEFGGQFIPEVLMPAIDELKEAYFELKDDEDFQNELSYYLKHYAGRETPLYYAENLTKKLGGAKIYLKREDLL
HGGAHKTNNTIGQALLAKRMGKKRIIAETGAGQHGVGTSMAGALFGLETEIFMGRLDTERQQPNVFRMKLLGAKVTPVDT
GSKVLKDAVNEAMRNWTATFENTHYLIGTVMGPHPFPTIVRDFQSVIGKEVKKQMTEQEGRFPDYLVACIGGGSNALGLF
HAFIPDKEVKMIGIEAAGKGLDTSLHGASISKGKKGVLHGMLSYFLQDEDGQIEEAYSISAGLDYPGIGPEHAYLHNSGR
AEYASATDEQALNALMELTRSEGIIPALESSHAVAYAIENAGTMDKDDIMVINLSGRGDKDLNTVIDIMQKRGM
>Mature_394_residues
MKCEGYFGEFGGQFIPEVLMPAIDELKEAYFELKDDEDFQNELSYYLKHYAGRETPLYYAENLTKKLGGAKIYLKREDLL
HGGAHKTNNTIGQALLAKRMGKKRIIAETGAGQHGVGTSMAGALFGLETEIFMGRLDTERQQPNVFRMKLLGAKVTPVDT
GSKVLKDAVNEAMRNWTATFENTHYLIGTVMGPHPFPTIVRDFQSVIGKEVKKQMTEQEGRFPDYLVACIGGGSNALGLF
HAFIPDKEVKMIGIEAAGKGLDTSLHGASISKGKKGVLHGMLSYFLQDEDGQIEEAYSISAGLDYPGIGPEHAYLHNSGR
AEYASATDEQALNALMELTRSEGIIPALESSHAVAYAIENAGTMDKDDIMVINLSGRGDKDLNTVIDIMQKRGM

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=392, Percent_Identity=56.8877551020408, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6321412, Length=388, Percent_Identity=52.8350515463918, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43186; Mature: 43186

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCEGYFGEFGGQFIPEVLMPAIDELKEAYFELKDDEDFQNELSYYLKHYAGRETPLYYA
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ENLTKKLGGAKIYLKREDLLHGGAHKTNNTIGQALLAKRMGKKRIIAETGAGQHGVGTSM
HHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
AGALFGLETEIFMGRLDTERQQPNVFRMKLLGAKVTPVDTGSKVLKDAVNEAMRNWTATF
HHHHHCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
ENTHYLIGTVMGPHPFPTIVRDFQSVIGKEVKKQMTEQEGRFPDYLVACIGGGSNALGLF
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HAFIPDKEVKMIGIEAAGKGLDTSLHGASISKGKKGVLHGMLSYFLQDEDGQIEEAYSIS
HHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHC
AGLDYPGIGPEHAYLHNSGRAEYASATDEQALNALMELTRSEGIIPALESSHAVAYAIEN
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
AGTMDKDDIMVINLSGRGDKDLNTVIDIMQKRGM
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKCEGYFGEFGGQFIPEVLMPAIDELKEAYFELKDDEDFQNELSYYLKHYAGRETPLYYA
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ENLTKKLGGAKIYLKREDLLHGGAHKTNNTIGQALLAKRMGKKRIIAETGAGQHGVGTSM
HHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
AGALFGLETEIFMGRLDTERQQPNVFRMKLLGAKVTPVDTGSKVLKDAVNEAMRNWTATF
HHHHHCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
ENTHYLIGTVMGPHPFPTIVRDFQSVIGKEVKKQMTEQEGRFPDYLVACIGGGSNALGLF
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HAFIPDKEVKMIGIEAAGKGLDTSLHGASISKGKKGVLHGMLSYFLQDEDGQIEEAYSIS
HHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHC
AGLDYPGIGPEHAYLHNSGRAEYASATDEQALNALMELTRSEGIIPALESSHAVAYAIEN
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
AGTMDKDDIMVINLSGRGDKDLNTVIDIMQKRGM
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3146017 [H]