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The map label for this gene is trpA

Identifier: 150399081

GI number: 150399081

Start: 376401

End: 377180

Strand: Reverse

Name: trpA

Synonym: Mevan_0327

Alternate gene names: 150399081

Gene position: 377180-376401 (Counterclockwise)

Preceding gene: 150399082

Following gene: 150399079

Centisome position: 21.93

GC content: 36.67

Gene sequence:

>780_bases
ATGAATAAACCTGTATTAGTTAGTTTTTTAGTTTCCGGGGACCCAAATGCAGACGCAACGTTAAAATTCATGAAAGCACT
TGATAAATATTCTGGCGTAATTGAACTCGGAATTCCATTTAGCGACCCCGTTGCAGATGGAGCTACAATTCAGGCAGCAG
ATGTCAGGGCATTATCAAACGGCTTTAAAATTGCTAAATCCTTTGAAATATTAAAGGAATTTAGAAAAGAGTCAAATACT
CCCGTAATTTTGATGACTTACTATAATCCAGTATACAACCGGGGAATTGAAAACTTTGTAACTCAGGCAAAAGAAGCAGG
AGCAAACGGCCTTATTATTGTTGATTTACCGCTACAGGAAGCAACAGAATATCGAAAAATCTGTAAAAAACACGAAATGG
GAACGGTATTTCTTTCAGCACCGAACACGCCAGAAGAACGTTTAAAAATGTCAGACGAAGCAAGTACTGAATTTTTATAC
CTTATTTCAACATTCGGGATAACTGGTGCAAGGGAATCGTTTGAACAGATGACATTTGACTTTATCAAAAGGGCGAGAAA
AACTTGTAAGGGAAAAATCTGTGTTGGATTTGGAATTTCAAAAGGAACTCACGCTGAAAGCTTGATTGAACAGGGTGCTG
ATGGAGTAATCGTTGGAAGTGCTTTTGTTGATATTATTAAAACATACGGGGACTCTAATGAAGCGATTACTAAATTAGAA
GAGCTCGCAAAAGAGTTGCATAATGGAATCGAAAAAGGATTTGAAAAAAGAAATAAATAA

Upstream 100 bases:

>100_bases
AACCATGGATAAGGATGATATAATGGTCATAAACCTTTCAGGGCGTGGCGACAAGGATTTAAACACGGTAATTGACATAA
TGCAAAAAAGAGGGATGTAA

Downstream 100 bases:

>100_bases
AAAATAAACAATAAATTAAAAATTTTAATATTTTATAATTTTAAAATATTGGGTGATTAAAAGAATTTATCAAGGGTAAT
CTGCCTATCGTTGCTTTTTA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNKPVLVSFLVSGDPNADATLKFMKALDKYSGVIELGIPFSDPVADGATIQAADVRALSNGFKIAKSFEILKEFRKESNT
PVILMTYYNPVYNRGIENFVTQAKEAGANGLIIVDLPLQEATEYRKICKKHEMGTVFLSAPNTPEERLKMSDEASTEFLY
LISTFGITGARESFEQMTFDFIKRARKTCKGKICVGFGISKGTHAESLIEQGADGVIVGSAFVDIIKTYGDSNEAITKLE
ELAKELHNGIEKGFEKRNK

Sequences:

>Translated_259_residues
MNKPVLVSFLVSGDPNADATLKFMKALDKYSGVIELGIPFSDPVADGATIQAADVRALSNGFKIAKSFEILKEFRKESNT
PVILMTYYNPVYNRGIENFVTQAKEAGANGLIIVDLPLQEATEYRKICKKHEMGTVFLSAPNTPEERLKMSDEASTEFLY
LISTFGITGARESFEQMTFDFIKRARKTCKGKICVGFGISKGTHAESLIEQGADGVIVGSAFVDIIKTYGDSNEAITKLE
ELAKELHNGIEKGFEKRNK
>Mature_259_residues
MNKPVLVSFLVSGDPNADATLKFMKALDKYSGVIELGIPFSDPVADGATIQAADVRALSNGFKIAKSFEILKEFRKESNT
PVILMTYYNPVYNRGIENFVTQAKEAGANGLIIVDLPLQEATEYRKICKKHEMGTVFLSAPNTPEERLKMSDEASTEFLY
LISTFGITGARESFEQMTFDFIKRARKTCKGKICVGFGISKGTHAESLIEQGADGVIVGSAFVDIIKTYGDSNEAITKLE
ELAKELHNGIEKGFEKRNK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=231, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321412, Length=259, Percent_Identity=39.3822393822394, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_METVS (A6UP14)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322848.1
- ProteinModelPortal:   A6UP14
- SMR:   A6UP14
- STRING:   A6UP14
- GeneID:   5325934
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0327
- eggNOG:   arNOG07707
- HOGENOM:   HBG391690
- OMA:   VILMTYY
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28526; Mature: 28526

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 35-35 ACT_SITE 46-46

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPVLVSFLVSGDPNADATLKFMKALDKYSGVIELGIPFSDPVADGATIQAADVRALSN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEHHHHHHHHH
GFKIAKSFEILKEFRKESNTPVILMTYYNPVYNRGIENFVTQAKEAGANGLIIVDLPLQE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCCCCEEEEECCHHH
ATEYRKICKKHEMGTVFLSAPNTPEERLKMSDEASTEFLYLISTFGITGARESFEQMTFD
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
FIKRARKTCKGKICVGFGISKGTHAESLIEQGADGVIVGSAFVDIIKTYGDSNEAITKLE
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHH
ELAKELHNGIEKGFEKRNK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKPVLVSFLVSGDPNADATLKFMKALDKYSGVIELGIPFSDPVADGATIQAADVRALSN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEHHHHHHHHH
GFKIAKSFEILKEFRKESNTPVILMTYYNPVYNRGIENFVTQAKEAGANGLIIVDLPLQE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCCCCEEEEECCHHH
ATEYRKICKKHEMGTVFLSAPNTPEERLKMSDEASTEFLYLISTFGITGARESFEQMTFD
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
FIKRARKTCKGKICVGFGISKGTHAESLIEQGADGVIVGSAFVDIIKTYGDSNEAITKLE
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHH
ELAKELHNGIEKGFEKRNK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA