Click here to switch to the map view.

The map label for this gene is 150399066

Identifier: 150399066

GI number: 150399066

Start: 363643

End: 364515

Strand: Reverse

Name: 150399066

Synonym: Mevan_0312

Alternate gene names: NA

Gene position: 364515-363643 (Counterclockwise)

Preceding gene: 150399067

Following gene: 150399065

Centisome position: 21.19

GC content: 30.81

Gene sequence:

>873_bases
ATGCGAATTATCGTGTTATACTCTGGAAATTACGGTGAACGGGTTTTAAATAATATTTTAACCAAATTTGCACCAAATAT
TATATCAGTTTATGAATTTGAAGAAAATTTACCCGAATATATCGAAGATATTGAAAATTACGTCCCAGATAATTTACCAG
AATGTGATTTACTGGTAGTAACTGGGTTACACGGCGATATTAATTTAGTTACCTGTAAAATTGCTAACAAAACCGGTGCA
AAATCTGTAATAATTGAATCACATTCTCAAAAGCAGTTACCGTTTGGCATACGATCTGAAATTACTGAAAGCCTTTTAAA
TGTAAAATGTGTATTTCCAAAGCCATTCTGTTCTTTGAAACCTGTTGGTGATATTTATATTGATGAATTTGTAAAATATT
TTGGAGCTCCAAAAATTGAAATAATCGGGGAAAAATTTATTAAATCAGTAATTGTACATAGAAATGCTCCTTGCGGTTCT
ACAAAATACATTGCAGAAAATTTAACTGGTTATCCATTAAATGAAGCAGAAATTGAGTCAGGAAATAAATTACATAATTA
CCCCTGCTTAGCATCGATGGAGTTTGATAACGAGTTTTCTGATACAATACTGCATCTTGCAGGTTATAAAATAAAAGAAG
CAGTTAAAAAATCTTTAGGATTTTCAAACATGGTTTTAAACGTAAATTCTAATTGCAAGGGGGTAGAATGCGGGTTTAAA
TGCTATAATATATGCCCCGTAGTTAAAATGGGTGAAAAGGCAGTAGAAATAATCGATAAGACTGCAAAAATTAATTATTT
ATACTGTGGCCTATGTATGAAATGTATTCGAGAATGCCCCTTTAATGCAATAATCTCTTTAAAGTATAGTTAA

Upstream 100 bases:

>100_bases
TTTTAAATTAGCGCCTGAAAAAATTGGGGAATTAAGCAATATATTGTACCTGAATATAATTTCAAAAAAATAATGAAATA
ATGGTTAACAGGTGGTTTAA

Downstream 100 bases:

>100_bases
TTTTTAAATAATAATATTTATTTTAAAAATAAATAGATTTTAACTAAATTAAGATTTAAAATATGCTATTTTTTCACATT
TTAGTATCAAGTTTAATGTT

Product: hypothetical protein

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIIVLYSGNYGERVLNNILTKFAPNIISVYEFEENLPEYIEDIENYVPDNLPECDLLVVTGLHGDINLVTCKIANKTGA
KSVIIESHSQKQLPFGIRSEITESLLNVKCVFPKPFCSLKPVGDIYIDEFVKYFGAPKIEIIGEKFIKSVIVHRNAPCGS
TKYIAENLTGYPLNEAEIESGNKLHNYPCLASMEFDNEFSDTILHLAGYKIKEAVKKSLGFSNMVLNVNSNCKGVECGFK
CYNICPVVKMGEKAVEIIDKTAKINYLYCGLCMKCIRECPFNAIISLKYS

Sequences:

>Translated_290_residues
MRIIVLYSGNYGERVLNNILTKFAPNIISVYEFEENLPEYIEDIENYVPDNLPECDLLVVTGLHGDINLVTCKIANKTGA
KSVIIESHSQKQLPFGIRSEITESLLNVKCVFPKPFCSLKPVGDIYIDEFVKYFGAPKIEIIGEKFIKSVIVHRNAPCGS
TKYIAENLTGYPLNEAEIESGNKLHNYPCLASMEFDNEFSDTILHLAGYKIKEAVKKSLGFSNMVLNVNSNCKGVECGFK
CYNICPVVKMGEKAVEIIDKTAKINYLYCGLCMKCIRECPFNAIISLKYS
>Mature_290_residues
MRIIVLYSGNYGERVLNNILTKFAPNIISVYEFEENLPEYIEDIENYVPDNLPECDLLVVTGLHGDINLVTCKIANKTGA
KSVIIESHSQKQLPFGIRSEITESLLNVKCVFPKPFCSLKPVGDIYIDEFVKYFGAPKIEIIGEKFIKSVIVHRNAPCGS
TKYIAENLTGYPLNEAEIESGNKLHNYPCLASMEFDNEFSDTILHLAGYKIKEAVKKSLGFSNMVLNVNSNCKGVECGFK
CYNICPVVKMGEKAVEIIDKTAKINYLYCGLCMKCIRECPFNAIISLKYS

Specific function: Is able to catalyze the biosynthesis of dTMP using dUMP, tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45). However, M.jannaschii like most methanogenic Archaea lacks

COG id: COG1810

COG function: function code S; Uncharacterized protein conserved in archaea

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003745 [H]

Pfam domain/function: PF02593 DUF166 [H]

EC number: 2.1.1.45

Molecular weight: Translated: 32516; Mature: 32516

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIVLYSGNYGERVLNNILTKFAPNIISVYEFEENLPEYIEDIENYVPDNLPECDLLVV
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TGLHGDINLVTCKIANKTGAKSVIIESHSQKQLPFGIRSEITESLLNVKCVFPKPFCSLK
ECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCC
PVGDIYIDEFVKYFGAPKIEIIGEKFIKSVIVHRNAPCGSTKYIAENLTGYPLNEAEIES
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHCC
GNKLHNYPCLASMEFDNEFSDTILHLAGYKIKEAVKKSLGFSNMVLNVNSNCKGVECGFK
CCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCE
CYNICPVVKMGEKAVEIIDKTAKINYLYCGLCMKCIRECPFNAIISLKYS
EECCCHHHHCCHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MRIIVLYSGNYGERVLNNILTKFAPNIISVYEFEENLPEYIEDIENYVPDNLPECDLLVV
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TGLHGDINLVTCKIANKTGAKSVIIESHSQKQLPFGIRSEITESLLNVKCVFPKPFCSLK
ECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCC
PVGDIYIDEFVKYFGAPKIEIIGEKFIKSVIVHRNAPCGSTKYIAENLTGYPLNEAEIES
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHCC
GNKLHNYPCLASMEFDNEFSDTILHLAGYKIKEAVKKSLGFSNMVLNVNSNCKGVECGFK
CCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCE
CYNICPVVKMGEKAVEIIDKTAKINYLYCGLCMKCIRECPFNAIISLKYS
EECCCHHHHCCHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]