Click here to switch to the map view.

The map label for this gene is aroE

Identifier: 150399007

GI number: 150399007

Start: 296617

End: 297471

Strand: Reverse

Name: aroE

Synonym: Mevan_0253

Alternate gene names: 150399007

Gene position: 297471-296617 (Counterclockwise)

Preceding gene: 150399008

Following gene: 150399006

Centisome position: 17.29

GC content: 28.54

Gene sequence:

>855_bases
TTGATAGATTCTAAAACAGTACTTCTTGGACTTATTGGCCATCCTGTTGAGCATTCTTTTTCACCAATTATGCACAATGC
TGCAATAAATGACTTAAAAATAAATTATGTGTATTTAGGATTTGATATTTCAAATGAAAATTTAAAAAATGTTGTAAATT
CTGCAAAAACACTCAGTATTTTGGGTTTTAATGTTACAATTCCGTATAAGGTAGAAATAATGAAGTACCTTGACAAAATC
GATGATACTGCAAAGTTTATTGGTGCTGTAAATACTGTAAAAATTGAAAATGGGGAGGCTGTTGGGTATAATACGGATGG
AATTGGTGCAAAAAAATCCCTTGAAGAAGAAATTGGCAAATTTAATAATAAAAATATATTAATGATTGGCTCAGGCGGTT
CTTCAAGATCAATTTCTTTTGAACTTGCAAAAGAGAATGAAATAACGATAATAAATAGAAATATAAAAAATGCAAAAGAA
TTGTCATTTGAAATTTCTAAGAATTTAGCTACTAAGGAGTATGGTGAAAAATACCTAAAATACGGCGATTTATCAACAGA
TATTTCAAAATTCGATATTATTATAAACACAACTCCAGTTGGAATGTACCCTGATGTTAATTCAAAGCCTGTAATTCCAT
TAAAAAATGCAAAAAATGATGCTATTATCATGGATATAATTTATAATCCTTTTGAACCAGTTTTTTTAAAAGAAGCTAAA
AAATATGGTTTAAAGACGTTAAATGGCCTTGGAATGCTTATTTACCAAGGTGCAGTTTCTTTTGAGATATGGACAGGATT
TAAACCAGATACTAACGTAATGAAAAACAGCATTGTTAATTTACTTAAAAGTTAA

Upstream 100 bases:

>100_bases
GGCGAAAATATATTGAAACGACAAGAAAATGCAGTAAATAAGGCTTTTGAGGCATTTATAAAAATATTAAATGAAGAATA
TAATATAAAGGAGTGATAAA

Downstream 100 bases:

>100_bases
TTCATAAATAATATAAATTTATAAATAAATAAAATTTTAGATTTTAAATTTAAAAAAATAGTTTTGGGAAAAGAACATGA
TAAAAGAAAGAGTTAAAATG

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MIDSKTVLLGLIGHPVEHSFSPIMHNAAINDLKINYVYLGFDISNENLKNVVNSAKTLSILGFNVTIPYKVEIMKYLDKI
DDTAKFIGAVNTVKIENGEAVGYNTDGIGAKKSLEEEIGKFNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKE
LSFEISKNLATKEYGEKYLKYGDLSTDISKFDIIINTTPVGMYPDVNSKPVIPLKNAKNDAIIMDIIYNPFEPVFLKEAK
KYGLKTLNGLGMLIYQGAVSFEIWTGFKPDTNVMKNSIVNLLKS

Sequences:

>Translated_284_residues
MIDSKTVLLGLIGHPVEHSFSPIMHNAAINDLKINYVYLGFDISNENLKNVVNSAKTLSILGFNVTIPYKVEIMKYLDKI
DDTAKFIGAVNTVKIENGEAVGYNTDGIGAKKSLEEEIGKFNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKE
LSFEISKNLATKEYGEKYLKYGDLSTDISKFDIIINTTPVGMYPDVNSKPVIPLKNAKNDAIIMDIIYNPFEPVFLKEAK
KYGLKTLNGLGMLIYQGAVSFEIWTGFKPDTNVMKNSIVNLLKS
>Mature_284_residues
MIDSKTVLLGLIGHPVEHSFSPIMHNAAINDLKINYVYLGFDISNENLKNVVNSAKTLSILGFNVTIPYKVEIMKYLDKI
DDTAKFIGAVNTVKIENGEAVGYNTDGIGAKKSLEEEIGKFNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKE
LSFEISKNLATKEYGEKYLKYGDLSTDISKFDIIINTTPVGMYPDVNSKPVIPLKNAKNDAIIMDIIYNPFEPVFLKEAK
KYGLKTLNGLGMLIYQGAVSFEIWTGFKPDTNVMKNSIVNLLKS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=274, Percent_Identity=31.3868613138686, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789675, Length=263, Percent_Identity=31.9391634980989, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320332, Length=282, Percent_Identity=30.1418439716312, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_METVS (A6UNU0)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322774.1
- ProteinModelPortal:   A6UNU0
- SMR:   A6UNU0
- STRING:   A6UNU0
- GeneID:   5324922
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0253
- eggNOG:   arNOG05588
- HOGENOM:   HBG553408
- OMA:   GYNTDAS
- ProtClustDB:   PRK00258
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 31504; Mature: 31504

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSKTVLLGLIGHPVEHSFSPIMHNAAINDLKINYVYLGFDISNENLKNVVNSAKTLSI
CCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEE
LGFNVTIPYKVEIMKYLDKIDDTAKFIGAVNTVKIENGEAVGYNTDGIGAKKSLEEEIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHC
FNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKELSFEISKNLATKEYGEKYLK
CCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
YGDLSTDISKFDIIINTTPVGMYPDVNSKPVIPLKNAKNDAIIMDIIYNPFEPVFLKEAK
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHH
KYGLKTLNGLGMLIYQGAVSFEIWTGFKPDTNVMKNSIVNLLKS
HHCCHHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDSKTVLLGLIGHPVEHSFSPIMHNAAINDLKINYVYLGFDISNENLKNVVNSAKTLSI
CCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEE
LGFNVTIPYKVEIMKYLDKIDDTAKFIGAVNTVKIENGEAVGYNTDGIGAKKSLEEEIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHC
FNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKELSFEISKNLATKEYGEKYLK
CCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
YGDLSTDISKFDIIINTTPVGMYPDVNSKPVIPLKNAKNDAIIMDIIYNPFEPVFLKEAK
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHH
KYGLKTLNGLGMLIYQGAVSFEIWTGFKPDTNVMKNSIVNLLKS
HHCCHHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA