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The map label for this gene is asnB [C]

Identifier: 150398966

GI number: 150398966

Start: 244392

End: 245936

Strand: Reverse

Name: asnB [C]

Synonym: Mevan_0211

Alternate gene names: 150398966

Gene position: 245936-244392 (Counterclockwise)

Preceding gene: 150398967

Following gene: 150398962

Centisome position: 14.3

GC content: 31.07

Gene sequence:

>1545_bases
ATGTGTTCTATAAGCGGAATTATTGCAAAAGACGGCGAAAGTACAAATGGCCGTGTTCTTATAAATAATCTTTCTAAACA
TTGCATAAATATGATGAAAATGCTAAAACACCGTGGGCCAGATAGTTCTGGAATAATCCTTGATGGTAACGTTTACTATT
TTAATAGTTTTGAAAATACGGATTTAGACTTTGATGGAACATTTAGACTTGGAATTTCACATAACAGACTTGCCATAGTT
GGAACTACAATTCAGCCAATTTCAAATCATGATAAATCAATATGGATTGTTTGTAACGGTGAAATATATAACCACGTTAA
ATTAAGGGAAAATTTAGCTACTGAACACGAATTTAAAACGGACAGTGATTCAGAAGTTATTATTCATTCATATGAGGATG
AATTAGTGGATATTCTTGATGGGGATTATGCATATGCCCTATACAATAAAGAAAAAAATTTATTAGAACTTAGGCGGGAT
TTAATGGGTGTAAAACCGCTTTTTTATCTCAATAGAAAAGATTATTTTGCATTTTCTTCTGAAAAAAAAGCACTATTTTA
CTGTCTTATGGAAATTGAAGGAATGGATTTTTACAGTGCATTTAATTTTAATGAAATACATCGGGTAAACCCTAATTCAC
GTATAATTTACGAATTAAACGAAAATAGCTATTATATTGAAGATAATCTTGAAAAGATAAATTCAAACTATTTTGAAGGT
GTAAATTATGAAAAATGTAAGGATGAACTTGAAAATGCACTTTTAGAATCTGTATTAAAGCGTGTAAACGGGATTGAAAA
AGTAGGAATTATCTATTCAGGTGGTGTTGATAGCACCCTTATATCAAAAATAGCATCAGAATTTTCAAATGTAACACTTT
ACTCCGTTGGAACAAAAGACTCTGAAGATTTAATTTATGCCGAGCGTGCTGCAATTGAAATGGGCCTAAAATTCAGGAAA
AAAATTATTTTAAAAGAAGATTACGAAAAATACCTTTTAAGCGTTGCACAGGCAATTGATGAACTAGACGTAATGAAATT
GAGTGTTGGAATTCCAATATATGTTGCATCACAAATGGCAAAAGAAGATGGAATAACTGTTGTACTGTCGGGTCAAGGTG
CAGATGAATTATTTGCAGGATATCATCGATACAAAAGAATATATGATGAAAATGGCGAAGAATATTTAAAAGAATCGATA
AAAAAAGATGTTTATAATATTTATAGGGCAAATCTTGAACGGGATGACCACGTAACAATGGCAAACGGCGTTGAATTGAG
GGTTCCATTTTTAGATAAAAAGGTAGTTGAAGTTTCGCTTTCAATTCCTGTTAAGTATAAAATTGAAGGCCAAGGAAAGA
AGATACTTAGAGAAATCGCTTTAAAATACATTCCAGAGTATATTGCAGAAAGACCGAAAAAAGCCGCACAGTATGGAAGT
GGCAGTGAAAAAATGGTTTACAGTGTTTCAAAAGATTATGGCTATTCAAAAAAAGAAATATCTAAATTTTTTGAAAACGT
ATTGATTGAGAAAATAAGATTTTAA

Upstream 100 bases:

>100_bases
AGATAATTTAATAATTTAATAATTAATTAGTTAATATTTAATAATGCATGAAAAGAAATAAAAATAAAAATACTTTTTTA
AGTGTTTAACGTGATTTAAA

Downstream 100 bases:

>100_bases
TATTTATTTATTTATTTATTTATTTATTTATTTTTAATTACATAATTTAAATTACCTTTCTAACTTATCCTTCTAGGCAT
CGCATCTCCTTTTCTTTTTT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MCSISGIIAKDGESTNGRVLINNLSKHCINMMKMLKHRGPDSSGIILDGNVYYFNSFENTDLDFDGTFRLGISHNRLAIV
GTTIQPISNHDKSIWIVCNGEIYNHVKLRENLATEHEFKTDSDSEVIIHSYEDELVDILDGDYAYALYNKEKNLLELRRD
LMGVKPLFYLNRKDYFAFSSEKKALFYCLMEIEGMDFYSAFNFNEIHRVNPNSRIIYELNENSYYIEDNLEKINSNYFEG
VNYEKCKDELENALLESVLKRVNGIEKVGIIYSGGVDSTLISKIASEFSNVTLYSVGTKDSEDLIYAERAAIEMGLKFRK
KIILKEDYEKYLLSVAQAIDELDVMKLSVGIPIYVASQMAKEDGITVVLSGQGADELFAGYHRYKRIYDENGEEYLKESI
KKDVYNIYRANLERDDHVTMANGVELRVPFLDKKVVEVSLSIPVKYKIEGQGKKILREIALKYIPEYIAERPKKAAQYGS
GSEKMVYSVSKDYGYSKKEISKFFENVLIEKIRF

Sequences:

>Translated_514_residues
MCSISGIIAKDGESTNGRVLINNLSKHCINMMKMLKHRGPDSSGIILDGNVYYFNSFENTDLDFDGTFRLGISHNRLAIV
GTTIQPISNHDKSIWIVCNGEIYNHVKLRENLATEHEFKTDSDSEVIIHSYEDELVDILDGDYAYALYNKEKNLLELRRD
LMGVKPLFYLNRKDYFAFSSEKKALFYCLMEIEGMDFYSAFNFNEIHRVNPNSRIIYELNENSYYIEDNLEKINSNYFEG
VNYEKCKDELENALLESVLKRVNGIEKVGIIYSGGVDSTLISKIASEFSNVTLYSVGTKDSEDLIYAERAAIEMGLKFRK
KIILKEDYEKYLLSVAQAIDELDVMKLSVGIPIYVASQMAKEDGITVVLSGQGADELFAGYHRYKRIYDENGEEYLKESI
KKDVYNIYRANLERDDHVTMANGVELRVPFLDKKVVEVSLSIPVKYKIEGQGKKILREIALKYIPEYIAERPKKAAQYGS
GSEKMVYSVSKDYGYSKKEISKFFENVLIEKIRF
>Mature_514_residues
MCSISGIIAKDGESTNGRVLINNLSKHCINMMKMLKHRGPDSSGIILDGNVYYFNSFENTDLDFDGTFRLGISHNRLAIV
GTTIQPISNHDKSIWIVCNGEIYNHVKLRENLATEHEFKTDSDSEVIIHSYEDELVDILDGDYAYALYNKEKNLLELRRD
LMGVKPLFYLNRKDYFAFSSEKKALFYCLMEIEGMDFYSAFNFNEIHRVNPNSRIIYELNENSYYIEDNLEKINSNYFEG
VNYEKCKDELENALLESVLKRVNGIEKVGIIYSGGVDSTLISKIASEFSNVTLYSVGTKDSEDLIYAERAAIEMGLKFRK
KIILKEDYEKYLLSVAQAIDELDVMKLSVGIPIYVASQMAKEDGITVVLSGQGADELFAGYHRYKRIYDENGEEYLKESI
KKDVYNIYRANLERDDHVTMANGVELRVPFLDKKVVEVSLSIPVKYKIEGQGKKILREIALKYIPEYIAERPKKAAQYGS
GSEKMVYSVSKDYGYSKKEISKFFENVLIEKIRF

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=516, Percent_Identity=28.2945736434109, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI168229252, Length=516, Percent_Identity=28.2945736434109, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI168229250, Length=516, Percent_Identity=28.2945736434109, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010848, Length=482, Percent_Identity=28.4232365145228, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI296010852, Length=405, Percent_Identity=27.9012345679012, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI296010850, Length=405, Percent_Identity=27.9012345679012, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI9506697, Length=314, Percent_Identity=26.1146496815287, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786889, Length=511, Percent_Identity=29.1585127201566, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71993933, Length=471, Percent_Identity=29.723991507431, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=471, Percent_Identity=29.723991507431, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17560178, Length=418, Percent_Identity=29.1866028708134, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25147560, Length=291, Percent_Identity=28.1786941580756, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321563, Length=518, Percent_Identity=28.957528957529, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325403, Length=519, Percent_Identity=27.5529865125241, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323542, Length=463, Percent_Identity=25.7019438444924, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI45553209, Length=524, Percent_Identity=27.4809160305344, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI221329991, Length=255, Percent_Identity=27.843137254902, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 59009; Mature: 59009

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSISGIIAKDGESTNGRVLINNLSKHCINMMKMLKHRGPDSSGIILDGNVYYFNSFENT
CCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCC
DLDFDGTFRLGISHNRLAIVGTTIQPISNHDKSIWIVCNGEIYNHVKLRENLATEHEFKT
CCCCCCEEEEECCCCEEEEEEEEECCCCCCCCEEEEEECCCEEEHHHHHHHHCCCCCCCC
DSDSEVIIHSYEDELVDILDGDYAYALYNKEKNLLELRRDLMGVKPLFYLNRKDYFAFSS
CCCCEEEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEECC
EKKALFYCLMEIEGMDFYSAFNFNEIHRVNPNSRIIYELNENSYYIEDNLEKINSNYFEG
CCCEEEEEEEEECCCCEEEECCCCCEEEECCCCEEEEEECCCCEEEECCHHHHCCHHHCC
VNYEKCKDELENALLESVLKRVNGIEKVGIIYSGGVDSTLISKIASEFSNVTLYSVGTKD
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCC
SEDLIYAERAAIEMGLKFRKKIILKEDYEKYLLSVAQAIDELDVMKLSVGIPIYVASQMA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHH
KEDGITVVLSGQGADELFAGYHRYKRIYDENGEEYLKESIKKDVYNIYRANLERDDHVTM
HCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ANGVELRVPFLDKKVVEVSLSIPVKYKIEGQGKKILREIALKYIPEYIAERPKKAAQYGS
ECCCEEEECCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GSEKMVYSVSKDYGYSKKEISKFFENVLIEKIRF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCSISGIIAKDGESTNGRVLINNLSKHCINMMKMLKHRGPDSSGIILDGNVYYFNSFENT
CCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCC
DLDFDGTFRLGISHNRLAIVGTTIQPISNHDKSIWIVCNGEIYNHVKLRENLATEHEFKT
CCCCCCEEEEECCCCEEEEEEEEECCCCCCCCEEEEEECCCEEEHHHHHHHHCCCCCCCC
DSDSEVIIHSYEDELVDILDGDYAYALYNKEKNLLELRRDLMGVKPLFYLNRKDYFAFSS
CCCCEEEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEECC
EKKALFYCLMEIEGMDFYSAFNFNEIHRVNPNSRIIYELNENSYYIEDNLEKINSNYFEG
CCCEEEEEEEEECCCCEEEECCCCCEEEECCCCEEEEEECCCCEEEECCHHHHCCHHHCC
VNYEKCKDELENALLESVLKRVNGIEKVGIIYSGGVDSTLISKIASEFSNVTLYSVGTKD
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCC
SEDLIYAERAAIEMGLKFRKKIILKEDYEKYLLSVAQAIDELDVMKLSVGIPIYVASQMA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHH
KEDGITVVLSGQGADELFAGYHRYKRIYDENGEEYLKESIKKDVYNIYRANLERDDHVTM
HCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ANGVELRVPFLDKKVVEVSLSIPVKYKIEGQGKKILREIALKYIPEYIAERPKKAAQYGS
ECCCEEEECCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GSEKMVYSVSKDYGYSKKEISKFFENVLIEKIRF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]