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The map label for this gene is nifK [H]

Identifier: 150398820

GI number: 150398820

Start: 59986

End: 61374

Strand: Reverse

Name: nifK [H]

Synonym: Mevan_0061

Alternate gene names: 150398820

Gene position: 61374-59986 (Counterclockwise)

Preceding gene: 150398821

Following gene: 150398819

Centisome position: 3.57

GC content: 39.74

Gene sequence:

>1389_bases
ATGAGTAATCTAAACGTAATCAACAAAAACAGGACCGCTGTAATCAACCCGCTCGTAACATGCCAACCATTAGGTGCAAT
GTTTGCAGTATCAGGAATTAAACGGGGACTTCCATTAGTTCACGGATCACAAGGATGTTCAACATTTGTAAGATACGGAT
TTGCAAGACATTTTAGGGAACCAGCTGATATCGCAGTAACTTCACTTCACGAAGATGCCGCAGTATTTGGTGGAAGAAAA
AATTTGATTTCGGGTATCGGAAATTTGGCAGCAAGATTCAAACCTGACGTCATGGGCGTAGTAACAACATGTTCAAGTGA
AATTATTGGGGATGATGTCGGTGGTTTCATTAAAATGGCCAAAGACGAAATTGTAAAGAAAATGGGCGTAACTGCCGCTG
AAAAAATAAAAATTGTCCAGATAAATACCCCAAGTTTTGTTGAACAGCACTACAAGGGATACGACAATGCAATAAAGGCA
ATTGTAGACACGCTCGCTGAACCAAAAGATGAACAGAACGAAAAAATAAACATCGTTCCTGGAATCGTAAACCCTGGTGA
CATTAGGGAAATTAAACACCTTCTTTCACTCATGAAAACAGAAGGAATCATGCTTACCGATACATCAGACCCATTTGACT
CACCACTCAGGCCTTCAAAAGCTGAAAAAAATCCCTACTACCAAAAAGGTGGAACTCCACTCGAAGAAATAAAAGATTCA
GCAAATTCGCTTGGTACAATATCACTCTGTAGTTATGCAAATACTGGTGCTCAGTCGCTTGAAAGAAAATACAAAATTCC
TTCAAAAGTGGGTGGAACTCCAATTGGCGTTCAAAATACTGATGAATTCGTAAGAACAGTTAAAAAATTCACTGGAAATG
AAGTTTCAGACGACCTTCTCGACGAAAGAGGATTATTAATTGACGCAATGGCCGACCTTTCATCAAGATACTTGTTTGGG
AGAAAAGTGGCAATCTACGGGGATCCTGCATTAACCGCAGGACTTGCAAGATTTGTAACGGAGCTTGGAATGATTCCTTC
AGTAGTTTGTGTCGGTGTTAAAAACCCGGAATTCGTTAAAGAAATGGAAAAAGTAGCTAAAGAATCAGAAGACGATGTAG
ATGTTCTCTTAGGACAGGATTTAAGAGCATTGGATGTTTACATAAAAGAAAACCCGGTAGACATCATGATAGGTAACTCA
GACGGTAGATTAATGGCAAAAGACCATAAAATACCGCTTTATAGGGTTGGATACCCAGTTTACGATAGAGTGGGAACTGC
AAGAAGGCCTATTGTTGGATACAACGGTGGCCTTAACTTGATTGACGGAATCACGAACACGATTCTTGACAAATACTACG
AAGTTCAAGACTGGAAACTCCAACAATAA

Upstream 100 bases:

>100_bases
TTGAAGGATTTGTGAATATGGCAAAAGACATATATTCAGCAATATATAACCCAGCCTGGAAATTGATGGAATTCCAAGAA
GAAGAGCCAGGTGATGCAAA

Downstream 100 bases:

>100_bases
GGAATCGATTGAACCGAGGAATACTATGGTTTTAAACCTGGCTACAGAAAACCGGAAAACTGAAATTTCAAGTAAAGACG
GTGATTTTGACATAGAGGTC

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSNLNVINKNRTAVINPLVTCQPLGAMFAVSGIKRGLPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRK
NLISGIGNLAARFKPDVMGVVTTCSSEIIGDDVGGFIKMAKDEIVKKMGVTAAEKIKIVQINTPSFVEQHYKGYDNAIKA
IVDTLAEPKDEQNEKINIVPGIVNPGDIREIKHLLSLMKTEGIMLTDTSDPFDSPLRPSKAEKNPYYQKGGTPLEEIKDS
ANSLGTISLCSYANTGAQSLERKYKIPSKVGGTPIGVQNTDEFVRTVKKFTGNEVSDDLLDERGLLIDAMADLSSRYLFG
RKVAIYGDPALTAGLARFVTELGMIPSVVCVGVKNPEFVKEMEKVAKESEDDVDVLLGQDLRALDVYIKENPVDIMIGNS
DGRLMAKDHKIPLYRVGYPVYDRVGTARRPIVGYNGGLNLIDGITNTILDKYYEVQDWKLQQ

Sequences:

>Translated_462_residues
MSNLNVINKNRTAVINPLVTCQPLGAMFAVSGIKRGLPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRK
NLISGIGNLAARFKPDVMGVVTTCSSEIIGDDVGGFIKMAKDEIVKKMGVTAAEKIKIVQINTPSFVEQHYKGYDNAIKA
IVDTLAEPKDEQNEKINIVPGIVNPGDIREIKHLLSLMKTEGIMLTDTSDPFDSPLRPSKAEKNPYYQKGGTPLEEIKDS
ANSLGTISLCSYANTGAQSLERKYKIPSKVGGTPIGVQNTDEFVRTVKKFTGNEVSDDLLDERGLLIDAMADLSSRYLFG
RKVAIYGDPALTAGLARFVTELGMIPSVVCVGVKNPEFVKEMEKVAKESEDDVDVLLGQDLRALDVYIKENPVDIMIGNS
DGRLMAKDHKIPLYRVGYPVYDRVGTARRPIVGYNGGLNLIDGITNTILDKYYEVQDWKLQQ
>Mature_461_residues
SNLNVINKNRTAVINPLVTCQPLGAMFAVSGIKRGLPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRKN
LISGIGNLAARFKPDVMGVVTTCSSEIIGDDVGGFIKMAKDEIVKKMGVTAAEKIKIVQINTPSFVEQHYKGYDNAIKAI
VDTLAEPKDEQNEKINIVPGIVNPGDIREIKHLLSLMKTEGIMLTDTSDPFDSPLRPSKAEKNPYYQKGGTPLEEIKDSA
NSLGTISLCSYANTGAQSLERKYKIPSKVGGTPIGVQNTDEFVRTVKKFTGNEVSDDLLDERGLLIDAMADLSSRYLFGR
KVAIYGDPALTAGLARFVTELGMIPSVVCVGVKNPEFVKEMEKVAKESEDDVDVLLGQDLRALDVYIKENPVDIMIGNSD
GRLMAKDHKIPLYRVGYPVYDRVGTARRPIVGYNGGLNLIDGITNTILDKYYEVQDWKLQQ

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50563; Mature: 50432

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLNVINKNRTAVINPLVTCQPLGAMFAVSGIKRGLPLVHGSQGCSTFVRYGFARHFRE
CCCCCEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCC
PADIAVTSLHEDAAVFGGRKNLISGIGNLAARFKPDVMGVVTTCSSEIIGDDVGGFIKMA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KDEIVKKMGVTAAEKIKIVQINTPSFVEQHYKGYDNAIKAIVDTLAEPKDEQNEKINIVP
HHHHHHHHCCCHHHCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
GIVNPGDIREIKHLLSLMKTEGIMLTDTSDPFDSPLRPSKAEKNPYYQKGGTPLEEIKDS
CCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ANSLGTISLCSYANTGAQSLERKYKIPSKVGGTPIGVQNTDEFVRTVKKFTGNEVSDDLL
HHCCCEEEEHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
DERGLLIDAMADLSSRYLFGRKVAIYGDPALTAGLARFVTELGMIPSVVCVGVKNPEFVK
HHCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
EMEKVAKESEDDVDVLLGQDLRALDVYIKENPVDIMIGNSDGRLMAKDHKIPLYRVGYPV
HHHHHHHCCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCCEEEECCCCCEEECCCCH
YDRVGTARRPIVGYNGGLNLIDGITNTILDKYYEVQDWKLQQ
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SNLNVINKNRTAVINPLVTCQPLGAMFAVSGIKRGLPLVHGSQGCSTFVRYGFARHFRE
CCCCEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCC
PADIAVTSLHEDAAVFGGRKNLISGIGNLAARFKPDVMGVVTTCSSEIIGDDVGGFIKMA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KDEIVKKMGVTAAEKIKIVQINTPSFVEQHYKGYDNAIKAIVDTLAEPKDEQNEKINIVP
HHHHHHHHCCCHHHCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
GIVNPGDIREIKHLLSLMKTEGIMLTDTSDPFDSPLRPSKAEKNPYYQKGGTPLEEIKDS
CCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ANSLGTISLCSYANTGAQSLERKYKIPSKVGGTPIGVQNTDEFVRTVKKFTGNEVSDDLL
HHCCCEEEEHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
DERGLLIDAMADLSSRYLFGRKVAIYGDPALTAGLARFVTELGMIPSVVCVGVKNPEFVK
HHCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
EMEKVAKESEDDVDVLLGQDLRALDVYIKENPVDIMIGNSDGRLMAKDHKIPLYRVGYPV
HHHHHHHCCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCCEEEECCCCCEEECCCCH
YDRVGTARRPIVGYNGGLNLIDGITNTILDKYYEVQDWKLQQ
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]