Click here to switch to the map view.

The map label for this gene is nifE [H]

Identifier: 150398819

GI number: 150398819

Start: 58530

End: 59960

Strand: Reverse

Name: nifE [H]

Synonym: Mevan_0060

Alternate gene names: 150398819

Gene position: 59960-58530 (Counterclockwise)

Preceding gene: 150398820

Following gene: 150398818

Centisome position: 3.49

GC content: 38.5

Gene sequence:

>1431_bases
ATGGTTTTAAACCTGGCTACAGAAAACCGGAAAACTGAAATTTCAAGTAAAGACGGTGATTTTGACATAGAGGTCAAAAT
CCCAAACTACATTATTGATACTTTAAAATCCCGTGAATCACACATGTGTGTTTCCGGTAAAAGCGACTCAATTCCAGACT
GCGACAAAAATTCAGCTCCGGGCATGATTACACAAAGATCCTGCGTATACGGTGGAGCTAGAGTCGTTTTAATGCCGATA
ACTGATGCAATACACCTCGTACACGGGCCAATAGGCTGTGCAGCATGTACATGGGATATTCGGGGAAGTACGTCTTCAGG
GGACAAACTTTATAAAACCGGATTTTCCACTGATTTACGGGAGAAAGACGTGGTATTTGGGGGAGAAAATAAATTATACG
AATCAATACTTGAATTAAATAGACTGTACACTCCTTCTGCAATATTTGTTTATTCTACTTGCGTAGTTGGCCTAATCGGG
GATGATATAAAAGCAGTATGCAAGAAAGCACAAGAAATAACAGGATGTAGGGTTATTCCGGTACAGTCAGAAGGGTTTAA
AAGCTTTAACAAGTCAGCAGGTCACAAACTTGCATGTGACGCAATGATTGATTATTTAATAGGTACAAAAGAACCAGAAG
AAGTTCACCCGTACAGTATTAATATAATTGGGGAATTCAACGTTGCAGGAGACCTCTTTGGAATTATACCATTATATGAA
AAAATGGGTGTAAAGGTACAGACTGCAATTACTGGGGATTCAACTGTAGAAAAAATTGCAAAATGCCACCATGCTAAGTT
AAACATAGTGCAGTGCCAGAAATCTTCCAATTATCTTGCAAGTAAAATGGAAGAGAAATACCAAATACCAACTATCAAGG
TAAATTTCTTTGGAGTGGAAGAAACTACGAAGTCTTTGAGGGCTGTTTCAGAGTTTTTCGGCGATGAAGAAATGATTAAA
AGAACAGAAGAACTCATAAACTCAGAAATTAAAAATCTTAGGGATGAAATTGCAAAATACAAAAAAGACCTTTCCGGAAA
GACTGTTGCAATATATTCTGGAGCCCATAAGTCCTGGGCACTTGTAAGCGCATTTTCAGAACTCGACATGGAAATTATCA
TGAGCGGTACTCAAAACGGAAAGCCCGAAGACTACCAAAAAATCAGGGAACACGTCTGCGAAGGCACATTAATTGTAGAC
GATGCAAATTCAATGGAACTTGCAAAGCTTTTAAAAGAATATAAGCCGGATATATTAATTTCTGGGGCTAAAGAAAAATA
TATTTCCCTAAAATGCGGGGTTCCGCACTGTGATTTCAATCACGATCGGATAACTGCGTTTTCAGGATATAACGGATTTA
TAAATTTTGCAAGAGTTGTGCATACGGCAGTTATGACGCCGGTTTGGAGACTTTCGAAGAAAATGACGTAG

Upstream 100 bases:

>100_bases
AACTTGATTGACGGAATCACGAACACGATTCTTGACAAATACTACGAAGTTCAAGACTGGAAACTCCAACAATAAGGAAT
CGATTGAACCGAGGAATACT

Downstream 100 bases:

>100_bases
AGGAATATTATGAATGATAAAAAATTTGCTGTAATAAATCCCTGTAAAATATGCCAGCCAATGGGGGCAATACTCGCATT
ACTTGGTGTGAAAAATGCAA

Product: nitrogenase MoFe cofactor biosynthesis protein NifE

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MVLNLATENRKTEISSKDGDFDIEVKIPNYIIDTLKSRESHMCVSGKSDSIPDCDKNSAPGMITQRSCVYGGARVVLMPI
TDAIHLVHGPIGCAACTWDIRGSTSSGDKLYKTGFSTDLREKDVVFGGENKLYESILELNRLYTPSAIFVYSTCVVGLIG
DDIKAVCKKAQEITGCRVIPVQSEGFKSFNKSAGHKLACDAMIDYLIGTKEPEEVHPYSINIIGEFNVAGDLFGIIPLYE
KMGVKVQTAITGDSTVEKIAKCHHAKLNIVQCQKSSNYLASKMEEKYQIPTIKVNFFGVEETTKSLRAVSEFFGDEEMIK
RTEELINSEIKNLRDEIAKYKKDLSGKTVAIYSGAHKSWALVSAFSELDMEIIMSGTQNGKPEDYQKIREHVCEGTLIVD
DANSMELAKLLKEYKPDILISGAKEKYISLKCGVPHCDFNHDRITAFSGYNGFINFARVVHTAVMTPVWRLSKKMT

Sequences:

>Translated_476_residues
MVLNLATENRKTEISSKDGDFDIEVKIPNYIIDTLKSRESHMCVSGKSDSIPDCDKNSAPGMITQRSCVYGGARVVLMPI
TDAIHLVHGPIGCAACTWDIRGSTSSGDKLYKTGFSTDLREKDVVFGGENKLYESILELNRLYTPSAIFVYSTCVVGLIG
DDIKAVCKKAQEITGCRVIPVQSEGFKSFNKSAGHKLACDAMIDYLIGTKEPEEVHPYSINIIGEFNVAGDLFGIIPLYE
KMGVKVQTAITGDSTVEKIAKCHHAKLNIVQCQKSSNYLASKMEEKYQIPTIKVNFFGVEETTKSLRAVSEFFGDEEMIK
RTEELINSEIKNLRDEIAKYKKDLSGKTVAIYSGAHKSWALVSAFSELDMEIIMSGTQNGKPEDYQKIREHVCEGTLIVD
DANSMELAKLLKEYKPDILISGAKEKYISLKCGVPHCDFNHDRITAFSGYNGFINFARVVHTAVMTPVWRLSKKMT
>Mature_476_residues
MVLNLATENRKTEISSKDGDFDIEVKIPNYIIDTLKSRESHMCVSGKSDSIPDCDKNSAPGMITQRSCVYGGARVVLMPI
TDAIHLVHGPIGCAACTWDIRGSTSSGDKLYKTGFSTDLREKDVVFGGENKLYESILELNRLYTPSAIFVYSTCVVGLIG
DDIKAVCKKAQEITGCRVIPVQSEGFKSFNKSAGHKLACDAMIDYLIGTKEPEEVHPYSINIIGEFNVAGDLFGIIPLYE
KMGVKVQTAITGDSTVEKIAKCHHAKLNIVQCQKSSNYLASKMEEKYQIPTIKVNFFGVEETTKSLRAVSEFFGDEEMIK
RTEELINSEIKNLRDEIAKYKKDLSGKTVAIYSGAHKSWALVSAFSELDMEIIMSGTQNGKPEDYQKIREHVCEGTLIVD
DANSMELAKLLKEYKPDILISGAKEKYISLKCGVPHCDFNHDRITAFSGYNGFINFARVVHTAVMTPVWRLSKKMT

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 52835; Mature: 52835

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLNLATENRKTEISSKDGDFDIEVKIPNYIIDTLKSRESHMCVSGKSDSIPDCDKNSAP
CEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
GMITQRSCVYGGARVVLMPITDAIHLVHGPIGCAACTWDIRGSTSSGDKLYKTGFSTDLR
CCEEECCEECCCCEEEEEECCHHHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCC
EKDVVFGGENKLYESILELNRLYTPSAIFVYSTCVVGLIGDDIKAVCKKAQEITGCRVIP
CCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
VQSEGFKSFNKSAGHKLACDAMIDYLIGTKEPEEVHPYSINIIGEFNVAGDLFGIIPLYE
ECCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KMGVKVQTAITGDSTVEKIAKCHHAKLNIVQCQKSSNYLASKMEEKYQIPTIKVNFFGVE
HCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEHH
ETTKSLRAVSEFFGDEEMIKRTEELINSEIKNLRDEIAKYKKDLSGKTVAIYSGAHKSWA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
LVSAFSELDMEIIMSGTQNGKPEDYQKIREHVCEGTLIVDDANSMELAKLLKEYKPDILI
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEE
SGAKEKYISLKCGVPHCDFNHDRITAFSGYNGFINFARVVHTAVMTPVWRLSKKMT
ECCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLNLATENRKTEISSKDGDFDIEVKIPNYIIDTLKSRESHMCVSGKSDSIPDCDKNSAP
CEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
GMITQRSCVYGGARVVLMPITDAIHLVHGPIGCAACTWDIRGSTSSGDKLYKTGFSTDLR
CCEEECCEECCCCEEEEEECCHHHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCC
EKDVVFGGENKLYESILELNRLYTPSAIFVYSTCVVGLIGDDIKAVCKKAQEITGCRVIP
CCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
VQSEGFKSFNKSAGHKLACDAMIDYLIGTKEPEEVHPYSINIIGEFNVAGDLFGIIPLYE
ECCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KMGVKVQTAITGDSTVEKIAKCHHAKLNIVQCQKSSNYLASKMEEKYQIPTIKVNFFGVE
HCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEHH
ETTKSLRAVSEFFGDEEMIKRTEELINSEIKNLRDEIAKYKKDLSGKTVAIYSGAHKSWA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
LVSAFSELDMEIIMSGTQNGKPEDYQKIREHVCEGTLIVDDANSMELAKLLKEYKPDILI
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEE
SGAKEKYISLKCGVPHCDFNHDRITAFSGYNGFINFARVVHTAVMTPVWRLSKKMT
ECCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]