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The map label for this gene is leuD

Identifier: 150394575

GI number: 150394575

Start: 2212687

End: 2213259

Strand: Direct

Name: leuD

Synonym: SaurJH1_2134

Alternate gene names: 150394575

Gene position: 2212687-2213259 (Clockwise)

Preceding gene: 150394574

Following gene: 150394576

Centisome position: 76.13

GC content: 33.68

Gene sequence:

>573_bases
ATGGCAGCAATCAAACCTATTACAACATATAAAGGTAAAATAGTCCCTCTCTTCAACGACAATATCGATACAGACCAAAT
CATTCCTAAGGTACACTTAAAGCGTATTTCAAAAAGTGGCTTTGGTCCATTTGCTTTTGATGAATGGCGGTACTTACCTG
ATGGTTCAGATAATCCTGATTTCAATCCTAACAAACCACAATATAAAGGGGCTTCTATTTTAATTACTGGAGATAATTTT
GGATGTGGTTCAAGTCGTGAACATGCTGCTTGGGCTCTTAAGGACTATGGTTTTCATATTATTATTGCAGGAAGTTTCAG
TGACATATTTTATATGAATTGCACTAAAAATGCGATGTTGCCTATCGTTTTAGAAAAAAGTGCTCGTGAACATCTTGCAC
AATATGAGGAAATTGAAATAGATTTACCAAATCAAACTGTGTCATCACCAGACAAGCGTTTCCATTTTGAAATTGATGAA
ACTTGGAAAAATAAACTTGTAAATGGCTTAGATGACATTGCAATCACCCTACAATATGAATCATTAATAGAAAAATATGA
AAAATCACTTTAA

Upstream 100 bases:

>100_bases
ACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCAGCAGCAGCTATTCATGGTAAATTTGTGGACG
TAAGAAAGGTGGTTGTTTAA

Downstream 100 bases:

>100_bases
GGGAGTTGAATATTATGACAGTCAAAACAACAGTTTCTACGAAAGATATCGATGAAGCATTTTTAAGACTTAAAGATATT
GTCAAAGAAACACCTTTACA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNF
GCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDE
TWKNKLVNGLDDIAITLQYESLIEKYEKSL

Sequences:

>Translated_190_residues
MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNF
GCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDE
TWKNKLVNGLDDIAITLQYESLIEKYEKSL
>Mature_189_residues
AAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFG
CGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDET
WKNKLVNGLDDIAITLQYESLIEKYEKSL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=186, Percent_Identity=45.1612903225806, Blast_Score=170, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=47.4747474747475, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_STAA1 (A7X4N4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442635.1
- ProteinModelPortal:   A7X4N4
- SMR:   A7X4N4
- STRING:   A7X4N4
- EnsemblBacteria:   EBSTAT00000002940
- GeneID:   5560047
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2045
- eggNOG:   COG0066
- GeneTree:   EBGT00050000024883
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   SAUR418127:SAHV_2045-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21623; Mature: 21491

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPD
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
FNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAML
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCEE
PIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDETWKNKLVNGLDDIAITLQYE
EEEECHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEHH
SLIEKYEKSL
HHHHHHHHCC
>Mature Secondary Structure 
AAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPD
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
FNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAML
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCEE
PIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDETWKNKLVNGLDDIAITLQYE
EEEECHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEHH
SLIEKYEKSL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA