Click here to switch to the map view.

The map label for this gene is leuA

Identifier: 150394572

GI number: 150394572

Start: 2208724

End: 2210253

Strand: Direct

Name: leuA

Synonym: SaurJH1_2131

Alternate gene names: 150394572

Gene position: 2208724-2210253 (Clockwise)

Preceding gene: 150394571

Following gene: 150394573

Centisome position: 75.99

GC content: 36.14

Gene sequence:

>1530_bases
ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGAGTGAATTTTACTTTTGATGA
ACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTAGATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTA
GCTTTAAATCTGTTCAAGCAATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATAGCAACATCACCTATTCATCT
TGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCATCTATTAAAGAACATGTCACATACGCGAAACAATTATTTG
ACGTTGTTCAATTTTCACCTGAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATATGCACATATTTTCAAAACCTT
AACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGTGCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATA
GTTTAGCTGCAATTGAAGGCGGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCCCTTAATCTCGAAGAAACTAA
AAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGAGTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTA
GTCATGAATCAGGTATTCACCAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAGTTAAAAGCATTAGGTTATAA
CATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTCAAGACCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAG
ATATTCATGCGATTATTCAAGGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTACAACTACAATATGTC
TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAGGATTCAAGTATTGGTACTGG
TTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTCCAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTG
TCACTGAAGGTACTGATGCCCAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGT
AGGTAATTAA

Upstream 100 bases:

>100_bases
AAATTGAAAAAGTTGGTCGTGAATTACGCGAAATGATGCCTTTTATTAAATCTAAAAGCATTGAAAAATAAGATAGACCT
ACAATGAGGAGTTGTTAAAT

Downstream 100 bases:

>100_bases
TTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAA
ATTATAAGTAATAAATATAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSD
IDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVG
VSTTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYV
SSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
DHDILQASCKAYVEAHAKFAAENVEKVGN

Sequences:

>Translated_509_residues
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSD
IDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVG
VSTTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYV
SSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
DHDILQASCKAYVEAHAKFAAENVEKVGN
>Mature_508_residues
SSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDI
DAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDA
GATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAAL
EEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGV
STTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVS
SGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGID
HDILQASCKAYVEAHAKFAAENVEKVGN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=495, Percent_Identity=43.8383838383838, Blast_Score=404, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=28.3068783068783, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6320071, Length=421, Percent_Identity=27.3159144893112, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=534, Percent_Identity=23.4082397003745, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=529, Percent_Identity=22.6843100189036, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_STAA1 (A7X4N1)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442632.1
- ProteinModelPortal:   A7X4N1
- SMR:   A7X4N1
- STRING:   A7X4N1
- EnsemblBacteria:   EBSTAT00000003756
- GeneID:   5558997
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2042
- eggNOG:   COG0119
- GeneTree:   EBGT00050000025207
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   SAUR418127:SAHV_2042-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55704; Mature: 55573

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQA
CCCCEEEEEEHHCCCCCCCCCCEEHHCCEEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
IAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHCCCHHHHHH
SIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEY
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
AHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCEECHHHHHHHHHCCHHH
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIH
HHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQF
CCCCCCCCCHHHCCCCEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
KTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQ
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
DSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCC
DHDILQASCKAYVEAHAKFAAENVEKVGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQA
CCCEEEEEEHHCCCCCCCCCCEEHHCCEEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
IAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEHHHHCCCHHHHHH
SIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEY
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
AHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAA
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCHHHHCCCCCEECHHHHHHHHHCCHHH
LEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIH
HHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQF
CCCCCCCCCHHHCCCCEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
KTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQ
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
DSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCC
DHDILQASCKAYVEAHAKFAAENVEKVGN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA