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The map label for this gene is dapE

Identifier: 150394543

GI number: 150394543

Start: 2175037

End: 2176260

Strand: Direct

Name: dapE

Synonym: SaurJH1_2101

Alternate gene names: 150394543

Gene position: 2175037-2176260 (Clockwise)

Preceding gene: 150394540

Following gene: 150394544

Centisome position: 74.83

GC content: 33.91

Gene sequence:

>1224_bases
ATGACAACTTTTAGTGAAAAAGAAAAAATTCAATTACTAGCAGATATTGTTGAACTACAAACTGAAAATAATAATGAAAT
AGACGTTTGTAATTATTTAAAAGATTTATTCGACAAGTACGATATTAAATCTGAAATTTTGAAAGTTAATGAACACCGCG
CCAATATCGTTGCAGAAATCGGTAACGGCTCACCTATACTCGCATTGAGTGGTCATATGGATGTTGTTGATGCAGGAAAT
CAAGATAATTGGACTTATCCCCCTTTTCAACTCACAGAAAAAGCTGGCAAATTATACGGTCGAGGCACAACGGATATGAA
AGGCGGTCTGATGGCTTTGGTCATCACACTCATTGAATTAAAAGAACAAAATCAATTGCCTCAAGGAACGATAAGATTAC
TAGCTACTGCTGGCGAAGAGAAAGAACAAGAAGGTGCCAAATTATTGGCTGATAAAGGCTATTTAGACGATGTAGATGGC
TTAATTATTGCTGAACCAACTGGATCTGGAATTTATTATGCACATAAAGGGTCTATGTCATGTAAAGTAACTGCAACTGG
CAAAGCTGTCCATAGCTCAGTTCCATTTATTGGTGACAATGCAATTGATACACTGCTTGAATTTTATAATCAATTTAAAG
AAAAATATTCAGAGCTTAAAAAACATGATACTAAACATGAATTAGATGTTGCACCCATGTTCAAATCATTGATAGGAAAA
GAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATGTTCGATTATAAATGGCGGCAAACAATTTAA
CTCTGTACCAGATGAAGCTTCACTTGAATTTAACGTAAGACCAGTTCCTGAGTATGATAACGACTTTATAGAATCGTTTT
TCCAAAATATCATTAATGATGTGGATAGCAATAAGCTTTCACTCGATATTCCAAGCAATCACCGACCTGTAACAAGCGAT
AAAAATAGCAAATTAATTACTACGATTAAAGATGTAGCTTCTAGTTATGTAGAACAAGACGAAATATTTGTTTCAGCGCT
TGTAGGTGCAACAGATGCCTCTAGTTTCTTAGGAGATAATAAGGACAATGTTGATTTAGCCATTTTTGGACCAGGTAATC
CATTAATGGCACATCAAATCGATGAATATATTGAAAAAGATATGTACCTGAAATATATTGATATTTTTAAAGAGGCTTCC
ATTCAATATTTAAAAGAGAAATAA

Upstream 100 bases:

>100_bases
AAATCAAATAACACGCTACTTTCTGCATTTTAAATTATGTTTAAGAATAAAAATTATGTTTATATAAATATATATACTAC
TTTGAAAAGGTGTGAGCTTA

Downstream 100 bases:

>100_bases
GAACGATGCTGTCAGCTGCCCTATTCGCGTGCTGGCAGTTTTTTATTGCGAATTTTAAGTTGATAGAAATAGCGATGAAT
ATTGTTTGACTGTAAATATT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGN
QDNWTYPPFQLTEKAGKLYGRGTTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTKHELDVAPMFKSLIGK
EISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSD
KNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEAS
IQYLKEK

Sequences:

>Translated_407_residues
MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGN
QDNWTYPPFQLTEKAGKLYGRGTTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTKHELDVAPMFKSLIGK
EISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSD
KNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEAS
IQYLKEK
>Mature_406_residues
TTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQ
DNWTYPPFQLTEKAGKLYGRGTTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGL
IIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTKHELDVAPMFKSLIGKE
ISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDK
NSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEASI
QYLKEK

Specific function: Displays A Broad Specificity And Can Also Deacylate Substrates Such As Acetylarginine, Acetylhistidine Or Acetylglutamate Semialdehyde. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family

Homologues:

Organism=Escherichia coli, GI1790395, Length=373, Percent_Identity=27.3458445040214, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788816, Length=340, Percent_Identity=23.2352941176471, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789236, Length=215, Percent_Identity=26.5116279069767, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_STAAN (Q99SN6)

Other databases:

- EMBL:   BA000018
- PIR:   E89991
- RefSeq:   NP_375115.1
- ProteinModelPortal:   Q99SN6
- STRING:   Q99SN6
- SWISS-2DPAGE:   Q99SN6
- EnsemblBacteria:   EBSTAT00000001306
- GeneID:   1124706
- GenomeReviews:   BA000018_GR
- KEGG:   sau:SA1814
- eggNOG:   COG0624
- GeneTree:   EBGT00050000024076
- HOGENOM:   HBG515002
- OMA:   KEQNELP
- ProtClustDB:   PRK08588
- BioCyc:   SAUR158879:SA1814-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001261
- InterPro:   IPR010182
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01910

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 45137; Mature: 45006

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 74-74 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNEHRANIVAEI
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEC
GNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRGTTDMKGGLMALVITLIEL
CCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLIIAEPTGSGIYYAHKGSMS
HHHCCCCHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCE
CKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTKHELDVAPMFKSLIGK
EEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCC
EISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIIND
HHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
VDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDN
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
KDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEASIQYLKEK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNEHRANIVAEI
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEC
GNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRGTTDMKGGLMALVITLIEL
CCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLIIAEPTGSGIYYAHKGSMS
HHHCCCCHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCE
CKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTKHELDVAPMFKSLIGK
EEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCC
EISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIIND
HHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
VDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDN
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
KDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEASIQYLKEK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146