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The map label for this gene is hlb [H]

Identifier: 150394540

GI number: 150394540

Start: 2171446

End: 2172270

Strand: Direct

Name: hlb [H]

Synonym: SaurJH1_2098

Alternate gene names: 150394540

Gene position: 2171446-2172270 (Clockwise)

Preceding gene: 150394539

Following gene: 150394543

Centisome position: 74.71

GC content: 33.58

Gene sequence:

>825_bases
ATGTATCCAAACTGGGGACAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAAT
ATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTACCAAACACCTG
TACTCGGTCGTTCTCAATCAGGTTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGCTGAAGATGGTGGCGTAGCG
ATTGTAAGTAAATATCCTATTAAAGAAAAAATCCAGCATGTTTTCAAAAGCGGTTGTGGATTCGATAATGATAGCAACAA
AGGCTTTGTTTATACAAAAATAGAGAAAAATGGTAAGAACATTCACGTTATCGGTACACATACACAATCTGAAGATTCAC
GTTGTGGTGCTGGACATGATCGAAAAATTAGAGCTGAACAAATGAAAGAAATCAGTGACTTTGTTAAAAAGAAAAATATC
CCTAAAGATGAAACGGTATATATAGGTGGCGACCTTAATGTTAATAAAGGCACTCCAGAGTTCAAAGATATGCTTAAAAA
CTTGAATGTAAATGATGTTCTATATGCAGGTCATAATAGCACATGGGACCCTCAATCAAATTCAATTGCGAAATATAATT
ACCCTAATGGTAAACCAGAACATTTAGACTATATATTTACAGATAAAGATCATAAACAACCAAAACAATTAGTCAATGAA
GTTGTGACTGAAAAACCTAAGCCATGGGATGTATATGCGTTCCCATATTACTACGTTTACAATGATTTTTCAGATCATTA
CCCAATCAAAGCCTATAGTAAATAG

Upstream 100 bases:

>100_bases
ATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAAT
ACTGTTGTTACAAGGTTTCT

Downstream 100 bases:

>100_bases
TGCTCAACTAACTAATAACTCGCTTCGTTCTAAAAGGACGAAGCGAGTTATATTGTTAAAATTTGAATTGACTTACATTT
TAATAAAATCATCTTAACAA

Product: phospholipase C

Products: NA

Alternate protein names: Beta-hemolysin; Beta-toxin; Sphingomyelinase; SMase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVA
IVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNIHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNI
PKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNE
VVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK

Sequences:

>Translated_274_residues
MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVA
IVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNIHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNI
PKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNE
VVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK
>Mature_274_residues
MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVA
IVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNIHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNI
PKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNE
VVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK

Specific function: Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Beta-hemolysin is a phospholipase C with specific activity toward sphingomyelins. Has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005135
- InterPro:   IPR017766 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 31270; Mature: 31270

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQS
CCCCCCCCHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKN
CCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCE
IHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPE
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCHH
FKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHH
VVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK
HHHCCCCCCEEEECEEEEEECCCCCCCCEEECCC
>Mature Secondary Structure
MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQS
CCCCCCCCHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
GWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKN
CCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCE
IHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPE
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCHH
FKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHH
VVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK
HHHCCCCCCEEEECEEEEEECCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8914839 [H]