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The map label for this gene is purA

Identifier: 150010348

GI number: 150010348

Start: 4610895

End: 4612166

Strand: Direct

Name: purA

Synonym: BDI_3782

Alternate gene names: 150010348

Gene position: 4610895-4612166 (Clockwise)

Preceding gene: 150010347

Following gene: 150010349

Centisome position: 95.83

GC content: 47.17

Gene sequence:

>1272_bases
ATGAAAGTAGACGTTTTGTTAGGATTGCAATGGGGCGACGAGGGCAAAGGCAAGGTTGTCGATGTGCTGACTCCGAATTA
CGATGTGGTAACTCGTTTTCAAGGAGGCCCTAATGCCGGACATACATTGGAGTTTAACGGGGAAAAGTATGTGTTACGTT
CGATTCCTTCCGGTATTTTCCAAGGTGGAAAGATAAACGTAATCGGTAACGGTGTCGTTCTGGACCCGTTATTGTTCAAG
CAGGAAGCGGAATCATTGGCAGCGAGTGGTCACGATATCACCAAGCAGCTTTATATCTCTAAGAAAGCTCACCTAATTTT
ACCTACTCACCGGATCTTGGATGCCGCTTACGAGGCCGCAAAAGGTTCCGGAAAGATCGGTACGACAGGAAAGGGTATCG
GCCCGACTTATACGGATAAGATTAGCCGTAACGGTGTTCGTGTGGGCGATTTGTTGCATAACTTCGAAGAGAAATATGCG
GCCGCTAAAGCAAAACATGAAGCGATCTTGCGTTCATTGAACTATGAATATGATATAACCGAGATGGAGGCTCAATGGCT
GGAGGCCTTGAACTACCTCAGACAATTCAAATTGATTGATAGCGAGCATGTCATCAATAACTATTTGAAGGAAGGCAAGT
CGGTCTTGGCGGAAGGCGCTCAAGGTACGATGCTGGATATAGACTTCGGTTCTTATCCATTCGTGACTTCCTCTAACACG
ATCTGTGCCGGATGTTGTACGGGACTGGGGGTTTCTCCCCGGAATATCGGTGAGGTATATGGAATCTTCAAGGCTTATTG
TACACGTGTAGGTAGCGGTCCGTTCCCGACCGAGTTGTTTGATGAGGTGGGAGATAAGATCGGTCAATTAGGCCATGAGT
TCGGTGCGGTTACGGGACGTAAACGCCGTTGTGGCTGGATTGATTTGGTAGCCTTGAGATATGCCGTGATGATCAACGGT
GTAAGCCAATTGATCATGATGAAGAGTGATGTGTTAGATACCTTCGATACGATCAAGGCTTGCGTGGCTTATAAGATGAA
CGGTGTCGAGACTAATGAGTTCCCGTATGAGATCGATGATACGATCGAACCTGTATACGTGGAATTACCGGGTTGGAAGA
CGGATATGACGAAGATGCGGAGCGAGGACGAGTTCCCTGAGGAGTTCAACGCTTACCTTTCTTTCTTGGAGGAAGAACTG
GGTGTGCCTGTGAAGATCGTTTCCGTAGGACCGGATCGTGAACAGACGATTGTCAGATATACAGAAGAATAA

Upstream 100 bases:

>100_bases
TTATTGTTTGTACATTTATGGCCTATGCAGTAAATGCAAGTTTAAGTTGCAAAGGTCAGTTAAATAAGAATATTAATAGC
CTATAATAAATATAGAAAAG

Downstream 100 bases:

>100_bases
ACTCGTTATAAATCGTACAAAATGGCAGAAGACCACATTCCTCAAGGGGTGTCTCCTGCCATTTTTGTCTTTGGCAAACT
TTTTGTCGTTGTTTTATAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPNYDVVTRFQGGPNAGHTLEFNGEKYVLRSIPSGIFQGGKINVIGNGVVLDPLLFK
QEAESLAASGHDITKQLYISKKAHLILPTHRILDAAYEAAKGSGKIGTTGKGIGPTYTDKISRNGVRVGDLLHNFEEKYA
AAKAKHEAILRSLNYEYDITEMEAQWLEALNYLRQFKLIDSEHVINNYLKEGKSVLAEGAQGTMLDIDFGSYPFVTSSNT
ICAGCCTGLGVSPRNIGEVYGIFKAYCTRVGSGPFPTELFDEVGDKIGQLGHEFGAVTGRKRRCGWIDLVALRYAVMING
VSQLIMMKSDVLDTFDTIKACVAYKMNGVETNEFPYEIDDTIEPVYVELPGWKTDMTKMRSEDEFPEEFNAYLSFLEEEL
GVPVKIVSVGPDREQTIVRYTEE

Sequences:

>Translated_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPNYDVVTRFQGGPNAGHTLEFNGEKYVLRSIPSGIFQGGKINVIGNGVVLDPLLFK
QEAESLAASGHDITKQLYISKKAHLILPTHRILDAAYEAAKGSGKIGTTGKGIGPTYTDKISRNGVRVGDLLHNFEEKYA
AAKAKHEAILRSLNYEYDITEMEAQWLEALNYLRQFKLIDSEHVINNYLKEGKSVLAEGAQGTMLDIDFGSYPFVTSSNT
ICAGCCTGLGVSPRNIGEVYGIFKAYCTRVGSGPFPTELFDEVGDKIGQLGHEFGAVTGRKRRCGWIDLVALRYAVMING
VSQLIMMKSDVLDTFDTIKACVAYKMNGVETNEFPYEIDDTIEPVYVELPGWKTDMTKMRSEDEFPEEFNAYLSFLEEEL
GVPVKIVSVGPDREQTIVRYTEE
>Mature_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPNYDVVTRFQGGPNAGHTLEFNGEKYVLRSIPSGIFQGGKINVIGNGVVLDPLLFK
QEAESLAASGHDITKQLYISKKAHLILPTHRILDAAYEAAKGSGKIGTTGKGIGPTYTDKISRNGVRVGDLLHNFEEKYA
AAKAKHEAILRSLNYEYDITEMEAQWLEALNYLRQFKLIDSEHVINNYLKEGKSVLAEGAQGTMLDIDFGSYPFVTSSNT
ICAGCCTGLGVSPRNIGEVYGIFKAYCTRVGSGPFPTELFDEVGDKIGQLGHEFGAVTGRKRRCGWIDLVALRYAVMING
VSQLIMMKSDVLDTFDTIKACVAYKMNGVETNEFPYEIDDTIEPVYVELPGWKTDMTKMRSEDEFPEEFNAYLSFLEEEL
GVPVKIVSVGPDREQTIVRYTEE

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=44.1588785046729, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI34577063, Length=433, Percent_Identity=45.2655889145497, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=42.6767676767677, Blast_Score=304, Evalue=9e-83,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=46.0992907801418, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=45.6876456876457, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=45.6876456876457, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=44.1588785046729, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=45.6264775413712, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PARD8 (A6LIF5)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001305091.1
- ProteinModelPortal:   A6LIF5
- SMR:   A6LIF5
- STRING:   A6LIF5
- GeneID:   5308931
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3782
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   PDIS435591:BDI_3782-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46775; Mature: 46775

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 221-221 BINDING 236-236 BINDING 300-300 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPNYDVVTRFQGGPNAGHTLEFNGEKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEHHHCCHHHC
QGGKINVIGNGVVLDPLLFKQEAESLAASGHDITKQLYISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHH
KGSGKIGTTGKGIGPTYTDKISRNGVRVGDLLHNFEEKYAAAKAKHEAILRSLNYEYDIT
CCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EMEAQWLEALNYLRQFKLIDSEHVINNYLKEGKSVLAEGAQGTMLDIDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCC
ICAGCCTGLGVSPRNIGEVYGIFKAYCTRVGSGPFPTELFDEVGDKIGQLGHEFGAVTGR
EEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KRRCGWIDLVALRYAVMINGVSQLIMMKSDVLDTFDTIKACVAYKMNGVETNEFPYEIDD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TIEPVYVELPGWKTDMTKMRSEDEFPEEFNAYLSFLEEELGVPVKIVSVGPDREQTIVRY
CCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
TEE
ECC
>Mature Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPNYDVVTRFQGGPNAGHTLEFNGEKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEHHHCCHHHC
QGGKINVIGNGVVLDPLLFKQEAESLAASGHDITKQLYISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHH
KGSGKIGTTGKGIGPTYTDKISRNGVRVGDLLHNFEEKYAAAKAKHEAILRSLNYEYDIT
CCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EMEAQWLEALNYLRQFKLIDSEHVINNYLKEGKSVLAEGAQGTMLDIDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCC
ICAGCCTGLGVSPRNIGEVYGIFKAYCTRVGSGPFPTELFDEVGDKIGQLGHEFGAVTGR
EEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KRRCGWIDLVALRYAVMINGVSQLIMMKSDVLDTFDTIKACVAYKMNGVETNEFPYEIDD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TIEPVYVELPGWKTDMTKMRSEDEFPEEFNAYLSFLEEELGVPVKIVSVGPDREQTIVRY
CCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
TEE
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA