Click here to switch to the map view.

The map label for this gene is ispH

Identifier: 150010306

GI number: 150010306

Start: 4555714

End: 4556586

Strand: Direct

Name: ispH

Synonym: BDI_3740

Alternate gene names: 150010306

Gene position: 4555714-4556586 (Clockwise)

Preceding gene: 150010305

Following gene: 150010307

Centisome position: 94.69

GC content: 52.0

Gene sequence:

>873_bases
ATGATAGAAGTCGAGATAGATAAAGATTCCGGATTCTGTTTTGGGGTTGTCACCGCCATAGAGAGCGCTGAGCGGGAGCT
GGGAAATACGGATACGCTGTATTGCCTAGGCGATATCGTTCATAACAGTTTGGAGGTGGAGCGGTTGGAGCATATGGGGC
TGCATACGATCGACCATGAGGGACTTTCAAGGTTGAGAGACCGGAAGGTGTTGCTCCGGGCGCATGGCGAGCCACCTTCT
ACCTATGCTTTAGCGAAGCGAAACAATATCACGATTATAGACGCTACTTGCCCGGTTGTCTTGCGTTTGCAGCGCAAGAT
CCATAAATGTTATCAAGAGACCCGGGCGAATAACACCCAGTTGGTTATCTATGGGAAGAAAGGCCATGCGGAGGTGAATG
GCTTGGTGGGGCAGACGGAGGGCACGGCGATTGTCATCGAGAAAATAGAAGACCTCGATCGTCTGGACTTTACCCGTGCG
ATCAGTCTGTTTTCCCAAACCACGAAGTCCTTGGATGGATTCAAGGCGGTAGTGGCGGAGATCAAGCAGCGGATGGCGGA
AGGGGTGGAGTTTAATTATTACGATACGATTTGCCGTCAGGTGGCGAATCGTCTTCCGAATATAAAAGCGTTCGCCTCCA
GCCACGATTGGGTCTATTTTGTGGCCGGTCGTAAAAGTTCCAACGGAAAGATGCTGTTCGAGGAGTGCCGGAAAGCGAAC
CCGAATACGCTTTTTATTTCCGAGGTATCAGAGATTACCGAGCCTTTGCCCGAGGGCGTGCGCCGGGTCGGGGTTTGTGG
CGCTACATCCACTCCCAAGTGGTTGATGGAGGAAGTGGCGGTCCGGATCAAGGAACTGAATGCTTCGGAATGA

Upstream 100 bases:

>100_bases
TCTTAGGGAAAACTCTAGTTTTGTTATGAAGAAACTATAGTTTCTTGTGATTGAAAATTAAGAAAACTGTAAGTGCTTGT
TTGTTAATGATATTATAGAT

Downstream 100 bases:

>100_bases
TATTTTGATTAAGATTACGTGAACTTTCCGGGAGAAAGACAGAAGGATAACTAAATATTTACTACTTTTGCCGGAGAACA
TTAAAATCAATGACGCTATG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIEVEIDKDSGFCFGVVTAIESAERELGNTDTLYCLGDIVHNSLEVERLEHMGLHTIDHEGLSRLRDRKVLLRAHGEPPS
TYALAKRNNITIIDATCPVVLRLQRKIHKCYQETRANNTQLVIYGKKGHAEVNGLVGQTEGTAIVIEKIEDLDRLDFTRA
ISLFSQTTKSLDGFKAVVAEIKQRMAEGVEFNYYDTICRQVANRLPNIKAFASSHDWVYFVAGRKSSNGKMLFEECRKAN
PNTLFISEVSEITEPLPEGVRRVGVCGATSTPKWLMEEVAVRIKELNASE

Sequences:

>Translated_290_residues
MIEVEIDKDSGFCFGVVTAIESAERELGNTDTLYCLGDIVHNSLEVERLEHMGLHTIDHEGLSRLRDRKVLLRAHGEPPS
TYALAKRNNITIIDATCPVVLRLQRKIHKCYQETRANNTQLVIYGKKGHAEVNGLVGQTEGTAIVIEKIEDLDRLDFTRA
ISLFSQTTKSLDGFKAVVAEIKQRMAEGVEFNYYDTICRQVANRLPNIKAFASSHDWVYFVAGRKSSNGKMLFEECRKAN
PNTLFISEVSEITEPLPEGVRRVGVCGATSTPKWLMEEVAVRIKELNASE
>Mature_290_residues
MIEVEIDKDSGFCFGVVTAIESAERELGNTDTLYCLGDIVHNSLEVERLEHMGLHTIDHEGLSRLRDRKVLLRAHGEPPS
TYALAKRNNITIIDATCPVVLRLQRKIHKCYQETRANNTQLVIYGKKGHAEVNGLVGQTEGTAIVIEKIEDLDRLDFTRA
ISLFSQTTKSLDGFKAVVAEIKQRMAEGVEFNYYDTICRQVANRLPNIKAFASSHDWVYFVAGRKSSNGKMLFEECRKAN
PNTLFISEVSEITEPLPEGVRRVGVCGATSTPKWLMEEVAVRIKELNASE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=287, Percent_Identity=29.616724738676, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PARD8 (A6LIB3)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001305049.1
- STRING:   A6LIB3
- GeneID:   5308889
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3740
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   AAIHFKR
- ProtClustDB:   PRK01045
- BioCyc:   PDIS435591:BDI_3740-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 32528; Mature: 32528

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVEIDKDSGFCFGVVTAIESAERELGNTDTLYCLGDIVHNSLEVERLEHMGLHTIDHE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCHHHHHHHHHCCCCCCCHH
GLSRLRDRKVLLRAHGEPPSTYALAKRNNITIIDATCPVVLRLQRKIHKCYQETRANNTQ
HHHHHHHHHEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
LVIYGKKGHAEVNGLVGQTEGTAIVIEKIEDLDRLDFTRAISLFSQTTKSLDGFKAVVAE
EEEEECCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQRMAEGVEFNYYDTICRQVANRLPNIKAFASSHDWVYFVAGRKSSNGKMLFEECRKAN
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHCCC
PNTLFISEVSEITEPLPEGVRRVGVCGATSTPKWLMEEVAVRIKELNASE
CCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIEVEIDKDSGFCFGVVTAIESAERELGNTDTLYCLGDIVHNSLEVERLEHMGLHTIDHE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCHHHHHHHHHCCCCCCCHH
GLSRLRDRKVLLRAHGEPPSTYALAKRNNITIIDATCPVVLRLQRKIHKCYQETRANNTQ
HHHHHHHHHEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
LVIYGKKGHAEVNGLVGQTEGTAIVIEKIEDLDRLDFTRAISLFSQTTKSLDGFKAVVAE
EEEEECCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQRMAEGVEFNYYDTICRQVANRLPNIKAFASSHDWVYFVAGRKSSNGKMLFEECRKAN
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHCCC
PNTLFISEVSEITEPLPEGVRRVGVCGATSTPKWLMEEVAVRIKELNASE
CCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA