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The map label for this gene is pfkA2 [H]

Identifier: 150010307

GI number: 150010307

Start: 4556684

End: 4557664

Strand: Direct

Name: pfkA2 [H]

Synonym: BDI_3741

Alternate gene names: 150010307

Gene position: 4556684-4557664 (Clockwise)

Preceding gene: 150010306

Following gene: 150010308

Centisome position: 94.71

GC content: 49.03

Gene sequence:

>981_bases
ATGTCTACAGTAAAGTGTGTTGGTATTTTAACGTCCGGAGGTGATGCCCCGGGAATGAATGCCGCTATTCGTGCGGTAAC
ACGTTCCGCTATTTATAACGGAATGAGAGTGAAAGGTATTTACCGTGGTTACAAAGGGTTGATCCTAGATGAGATTGAAG
AGTTTAAGACACAAAATGTCAGTAATATAATTCAGCGCGGCGGAACAATCCTTAAGACCGCCCGTTGCGTGGAGTTCAAG
ACTCCCGAGGGACGAAAACAGGCCTATGATAAGTTGCAAGAGCATGGGATCGATGCCTTGATCGCTATCGGTGGTGACGG
AACGTTGACGGGAGCGCGTATTTTCGCTCAGGAGTTTAATTTCCCGATCGTAGGATTGCCGGGTACGATTGATAATGACT
TGTATGGCACGGATACGACGATCGGTTATGATACGGCCTTGAACACGATCATGGAATGCGTGGATAAGATCCGTGATACG
GCTACCTCGCATGATCGTCTATTTTTTGTGGAGGTCATGGGGCGCGACGCCGGTTTCTTGGCGTTGAACGGAGCGATCGC
TTCTGGTGCCGAAGCCGCTATTATCCCGGAAATCTCCTTGGAGAAAGATCAATTGGCCGAGATGATCGAGAATGGATTCC
GTAAGTCCAAGAACAGTAGTATCGTATTGGTTGCCGAAAGTGAGGTAACCGGTGGCGCTATGGGTGTCGCTGAGCGTGTG
AAGAAGGAATATCCGCAGTTCGATGTGCGTGTCTCTATTTTAGGTCACTTGCAGCGTGGTGGTTCTCCTACGGCACAAGA
CCGGATCTTGGCGACTCGTATGGGTGTGGCCGCGATCGATGCCTTGTTGGATGACCAGCGTAACGTGATGATGGGTATCC
AGAACGACCAGATCGTCTATGTACCGTTCTCTAAAGCGATCAAGAATGATAAGCCTATTAACCGGGATTTGTTGAACACG
CTCCGTGTTTCTTCAATCTAA

Upstream 100 bases:

>100_bases
TGATATTTTGATTAAGATTACGTGAACTTTCCGGGAGAAAGACAGAAGGATAACTAAATATTTACTACTTTTGCCGGAGA
ACATTAAAATCAATGACGCT

Downstream 100 bases:

>100_bases
ATAAGGCATATATTTATACGAATAGGAACCCGGATGTAGCGATTGCGTACATCCGGGTTTTTATTTGAAGAACCTGATAT
CATGAAGAAGTATAGCGGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSTVKCVGILTSGGDAPGMNAAIRAVTRSAIYNGMRVKGIYRGYKGLILDEIEEFKTQNVSNIIQRGGTILKTARCVEFK
TPEGRKQAYDKLQEHGIDALIAIGGDGTLTGARIFAQEFNFPIVGLPGTIDNDLYGTDTTIGYDTALNTIMECVDKIRDT
ATSHDRLFFVEVMGRDAGFLALNGAIASGAEAAIIPEISLEKDQLAEMIENGFRKSKNSSIVLVAESEVTGGAMGVAERV
KKEYPQFDVRVSILGHLQRGGSPTAQDRILATRMGVAAIDALLDDQRNVMMGIQNDQIVYVPFSKAIKNDKPINRDLLNT
LRVSSI

Sequences:

>Translated_326_residues
MSTVKCVGILTSGGDAPGMNAAIRAVTRSAIYNGMRVKGIYRGYKGLILDEIEEFKTQNVSNIIQRGGTILKTARCVEFK
TPEGRKQAYDKLQEHGIDALIAIGGDGTLTGARIFAQEFNFPIVGLPGTIDNDLYGTDTTIGYDTALNTIMECVDKIRDT
ATSHDRLFFVEVMGRDAGFLALNGAIASGAEAAIIPEISLEKDQLAEMIENGFRKSKNSSIVLVAESEVTGGAMGVAERV
KKEYPQFDVRVSILGHLQRGGSPTAQDRILATRMGVAAIDALLDDQRNVMMGIQNDQIVYVPFSKAIKNDKPINRDLLNT
LRVSSI
>Mature_325_residues
STVKCVGILTSGGDAPGMNAAIRAVTRSAIYNGMRVKGIYRGYKGLILDEIEEFKTQNVSNIIQRGGTILKTARCVEFKT
PEGRKQAYDKLQEHGIDALIAIGGDGTLTGARIFAQEFNFPIVGLPGTIDNDLYGTDTTIGYDTALNTIMECVDKIRDTA
TSHDRLFFVEVMGRDAGFLALNGAIASGAEAAIIPEISLEKDQLAEMIENGFRKSKNSSIVLVAESEVTGGAMGVAERVK
KEYPQFDVRVSILGHLQRGGSPTAQDRILATRMGVAAIDALLDDQRNVMMGIQNDQIVYVPFSKAIKNDKPINRDLLNTL
RVSSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=42.9394812680115, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=41.6666666666667, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI266453768, Length=347, Percent_Identity=40.0576368876081, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI266453748, Length=347, Percent_Identity=40.0576368876081, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI4505749, Length=347, Percent_Identity=40.0576368876081, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI266453619, Length=347, Percent_Identity=40.0576368876081, Blast_Score=235, Evalue=4e-62,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=47.1875, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25147584, Length=351, Percent_Identity=41.3105413105413, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17558788, Length=306, Percent_Identity=39.2156862745098, Blast_Score=203, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=33.1034482758621, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=40.2332361516035, Blast_Score=232, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=37.9710144927536, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI28573326, Length=352, Percent_Identity=41.4772727272727, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24652337, Length=347, Percent_Identity=41.4985590778098, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=41.4985590778098, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35307; Mature: 35176

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVKCVGILTSGGDAPGMNAAIRAVTRSAIYNGMRVKGIYRGYKGLILDEIEEFKTQNV
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCCHHHHHHHHHHHCCH
SNIIQRGGTILKTARCVEFKTPEGRKQAYDKLQEHGIDALIAIGGDGTLTGARIFAQEFN
HHHHHCCCCEEEHEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC
FPIVGLPGTIDNDLYGTDTTIGYDTALNTIMECVDKIRDTATSHDRLFFVEVMGRDAGFL
CCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
ALNGAIASGAEAAIIPEISLEKDQLAEMIENGFRKSKNSSIVLVAESEVTGGAMGVAERV
EECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
KKEYPQFDVRVSILGHLQRGGSPTAQDRILATRMGVAAIDALLDDQRNVMMGIQNDQIVY
HHHCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEE
VPFSKAIKNDKPINRDLLNTLRVSSI
EECHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
STVKCVGILTSGGDAPGMNAAIRAVTRSAIYNGMRVKGIYRGYKGLILDEIEEFKTQNV
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCCHHHHHHHHHHHCCH
SNIIQRGGTILKTARCVEFKTPEGRKQAYDKLQEHGIDALIAIGGDGTLTGARIFAQEFN
HHHHHCCCCEEEHEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC
FPIVGLPGTIDNDLYGTDTTIGYDTALNTIMECVDKIRDTATSHDRLFFVEVMGRDAGFL
CCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
ALNGAIASGAEAAIIPEISLEKDQLAEMIENGFRKSKNSSIVLVAESEVTGGAMGVAERV
EECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
KKEYPQFDVRVSILGHLQRGGSPTAQDRILATRMGVAAIDALLDDQRNVMMGIQNDQIVY
HHHCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEE
VPFSKAIKNDKPINRDLLNTLRVSSI
EECHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]