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The map label for this gene is gltD [H]

Identifier: 150010256

GI number: 150010256

Start: 4504665

End: 4506140

Strand: Direct

Name: gltD [H]

Synonym: BDI_3682

Alternate gene names: 150010256

Gene position: 4504665-4506140 (Clockwise)

Preceding gene: 150010255

Following gene: 150010266

Centisome position: 93.63

GC content: 51.36

Gene sequence:

>1476_bases
ATGACAACAGAAGAATTAATCGCTGCTCGTCGTGCCGAACCTTGGCGGGAGGCACTTCGCAGAAGCAAGAAAAATAAAGA
ACGTGCGGATATCCCCCGTGTGAAGATGAACGAGTTGGACCCGGAATACCGGAGTCGTACCCGTTTGGAGGAAGTTAACT
TAGGCTTGACAAAAGAACAGGCCATGCAAGAGGCCGAGCGCTGTTTGGATTGTCCGAATCCTACTTGTATGCAGGGATGT
CCGGTAAATATCAATATACCTACTTTTATCAAGAATATCGAGCGTGGGGAATTCTTGGAGGCCGCTAAGACCTTGAAAGA
GACAAGTGCCCTGCCCGCTGTTTGCGGTCGTGTCTGTCCGCAGGAGAAACAATGCGAGAGCAAGTGTATCCATTTGAAAA
TGGGTAAGGAAGCGGTTGCCATTGGCTACTTGGAGCGTTTCGCCGCTGATTACGAACGTGAGAGCGGTAATATCTCGGTT
CCGGAGATCGCGGAGAAGAACGGTATCAAGATTGCCGTGGTAGGTTCCGGTCCTGCCGGATTGTCTTTTGCCGGAGATAT
GGCGAAGCGTGGATATGATGTGACTGTGTTCGAGGCATTGCATGAGATCGGTGGTGTATTGAAATATGGTATCCCGGAGT
TCCGCCTACCGAATAAGATCGTGGACGTGGAGATTGATGGTCTTCGGAAGATGGGCGTACAGTTTGAGAAAGATTGTATC
GTGGGCAAGACGATCAGTTATGATGATTTGCATGCGGATGGCTTTAAGGGCGTGTTTGTGGCAAGTGGCGCCGGTCTGCC
TAACTTTATGAACATTCCGGGTGAGAACTTTGTGGGCGTGATGTCGTCCAACGAGTATTTGACCCGTGTAAACTTAATGG
ATGCGGCGAATCCGGAAAGTGATACGCCGGTGCTACAAGGAAAGAAGGTGGCCGTGATCGGTGGAGGAAATACCGCTATG
GACTCTGTCCGTACCGCTCGTCGTCTGGGTGCTGAGCGTGCTATGATCGTCTACCGTCGTAGCGAGGAGGAGATGCCTGC
CCGTCTGGAAGAGGTGAAACATGCGAAGGAAGAAGGTGTGGAGTTCTTGACGCTTCATAATCCGATTGAATATATAGGTG
ATGAAAGAGGCCGTGTGAAACAGATGCGTTTGCAGAAGATGGAATTGGGCGAGCCGGATGCCTCTGGTCGCCGTCGTCCG
GTACCTATCGAGGGCGCTATCGAAACAATTGATGTGGATGAGGTGATCGTTAGCGTCGGTGTATCTCCGAATCCATTAAT
ACCCCGTGCCTTTGGAGGCTTGGAGGTGAGCAAGAAGGGTACGATCGTAGTGGAGGAAGACAGTATGCGTTCCGCTTTAG
GCGATGTGTATGCCGGTGGTGACATCGTTCGTGGTGGAGCTACCGTGATTCTGGCGATGGGAGACGGTCGTAAGGCCGCC
GCCGCTATGGATGCGGACTTAAAAGGAAAAGTATAA

Upstream 100 bases:

>100_bases
CGGAGTTCGATGCTCATCAAGTGGATTTTGATGAGATGATCATGCGTCTCGGCGCTTATAAGGATATAGAGAAGAAATAA
CGAAACCACGTAATTGAATC

Downstream 100 bases:

>100_bases
TATCTATTAATTCAATAAGGAAAGACGGGTTGTTTAAACGATCCGTCTTTTTTCTTTTATCGTGATACGGAGATTTATGT
TTATTATCTATAAGACTAAT

Product: putative NADPH-dependent glutamate synthase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTTEELIAARRAEPWREALRRSKKNKERADIPRVKMNELDPEYRSRTRLEEVNLGLTKEQAMQEAERCLDCPNPTCMQGC
PVNINIPTFIKNIERGEFLEAAKTLKETSALPAVCGRVCPQEKQCESKCIHLKMGKEAVAIGYLERFAADYERESGNISV
PEIAEKNGIKIAVVGSGPAGLSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIDGLRKMGVQFEKDCI
VGKTISYDDLHADGFKGVFVASGAGLPNFMNIPGENFVGVMSSNEYLTRVNLMDAANPESDTPVLQGKKVAVIGGGNTAM
DSVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGVEFLTLHNPIEYIGDERGRVKQMRLQKMELGEPDASGRRRP
VPIEGAIETIDVDEVIVSVGVSPNPLIPRAFGGLEVSKKGTIVVEEDSMRSALGDVYAGGDIVRGGATVILAMGDGRKAA
AAMDADLKGKV

Sequences:

>Translated_491_residues
MTTEELIAARRAEPWREALRRSKKNKERADIPRVKMNELDPEYRSRTRLEEVNLGLTKEQAMQEAERCLDCPNPTCMQGC
PVNINIPTFIKNIERGEFLEAAKTLKETSALPAVCGRVCPQEKQCESKCIHLKMGKEAVAIGYLERFAADYERESGNISV
PEIAEKNGIKIAVVGSGPAGLSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIDGLRKMGVQFEKDCI
VGKTISYDDLHADGFKGVFVASGAGLPNFMNIPGENFVGVMSSNEYLTRVNLMDAANPESDTPVLQGKKVAVIGGGNTAM
DSVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGVEFLTLHNPIEYIGDERGRVKQMRLQKMELGEPDASGRRRP
VPIEGAIETIDVDEVIVSVGVSPNPLIPRAFGGLEVSKKGTIVVEEDSMRSALGDVYAGGDIVRGGATVILAMGDGRKAA
AAMDADLKGKV
>Mature_490_residues
TTEELIAARRAEPWREALRRSKKNKERADIPRVKMNELDPEYRSRTRLEEVNLGLTKEQAMQEAERCLDCPNPTCMQGCP
VNINIPTFIKNIERGEFLEAAKTLKETSALPAVCGRVCPQEKQCESKCIHLKMGKEAVAIGYLERFAADYERESGNISVP
EIAEKNGIKIAVVGSGPAGLSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIDGLRKMGVQFEKDCIV
GKTISYDDLHADGFKGVFVASGAGLPNFMNIPGENFVGVMSSNEYLTRVNLMDAANPESDTPVLQGKKVAVIGGGNTAMD
SVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGVEFLTLHNPIEYIGDERGRVKQMRLQKMELGEPDASGRRRPV
PIEGAIETIDVDEVIVSVGVSPNPLIPRAFGGLEVSKKGTIVVEEDSMRSALGDVYAGGDIVRGGATVILAMGDGRKAAA
AMDADLKGKV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=445, Percent_Identity=31.0112359550562, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1789606, Length=432, Percent_Identity=36.8055555555556, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788811, Length=488, Percent_Identity=35.2459016393443, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082180, Length=485, Percent_Identity=35.0515463917526, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=34.1573033707865, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1789243, Length=424, Percent_Identity=33.2547169811321, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17570289, Length=478, Percent_Identity=29.2887029288703, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71984108, Length=448, Percent_Identity=29.9107142857143, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17543860, Length=247, Percent_Identity=26.7206477732794, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=497, Percent_Identity=30.9859154929577, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24640763, Length=497, Percent_Identity=30.3822937625755, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI18858217, Length=497, Percent_Identity=30.3822937625755, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24665547, Length=502, Percent_Identity=27.6892430278884, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24665543, Length=502, Percent_Identity=27.6892430278884, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=27.6341948310139, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=27.6341948310139, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53652; Mature: 53521

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEELIAARRAEPWREALRRSKKNKERADIPRVKMNELDPEYRSRTRLEEVNLGLTKEQ
CCHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHCCCHHHHHHHHHHHHCCCCCHHH
AMQEAERCLDCPNPTCMQGCPVNINIPTFIKNIERGEFLEAAKTLKETSALPAVCGRVCP
HHHHHHHHHCCCCCCHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCC
QEKQCESKCIHLKMGKEAVAIGYLERFAADYERESGNISVPEIAEKNGIKIAVVGSGPAG
CHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEECCCCC
LSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIDGLRKMGVQFEKDCI
CCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCEECCCEE
VGKTISYDDLHADGFKGVFVASGAGLPNFMNIPGENFVGVMSSNEYLTRVNLMDAANPES
ECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHCCCCCC
DTPVLQGKKVAVIGGGNTAMDSVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGV
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
EFLTLHNPIEYIGDERGRVKQMRLQKMELGEPDASGRRRPVPIEGAIETIDVDEVIVSVG
EEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHHHCCC
VSPNPLIPRAFGGLEVSKKGTIVVEEDSMRSALGDVYAGGDIVRGGATVILAMGDGRKAA
CCCCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHCCCCEEECCEEEEEEECCCCCHH
AAMDADLKGKV
HHHCCCCCCCC
>Mature Secondary Structure 
TTEELIAARRAEPWREALRRSKKNKERADIPRVKMNELDPEYRSRTRLEEVNLGLTKEQ
CHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCHHCCCHHHHHHHHHHHHCCCCCHHH
AMQEAERCLDCPNPTCMQGCPVNINIPTFIKNIERGEFLEAAKTLKETSALPAVCGRVCP
HHHHHHHHHCCCCCCHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCC
QEKQCESKCIHLKMGKEAVAIGYLERFAADYERESGNISVPEIAEKNGIKIAVVGSGPAG
CHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEECCCCC
LSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIDGLRKMGVQFEKDCI
CCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCEECCCEE
VGKTISYDDLHADGFKGVFVASGAGLPNFMNIPGENFVGVMSSNEYLTRVNLMDAANPES
ECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHCCCCCC
DTPVLQGKKVAVIGGGNTAMDSVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGV
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
EFLTLHNPIEYIGDERGRVKQMRLQKMELGEPDASGRRRPVPIEGAIETIDVDEVIVSVG
EEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHHHCCC
VSPNPLIPRAFGGLEVSKKGTIVVEEDSMRSALGDVYAGGDIVRGGATVILAMGDGRKAA
CCCCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHCCCCEEECCEEEEEEECCCCCHH
AAMDADLKGKV
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]