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The map label for this gene is rfbB [H]

Identifier: 150010193

GI number: 150010193

Start: 4428976

End: 4430118

Strand: Direct

Name: rfbB [H]

Synonym: BDI_3616

Alternate gene names: 150010193

Gene position: 4428976-4430118 (Clockwise)

Preceding gene: 150010192

Following gene: 150010194

Centisome position: 92.05

GC content: 41.64

Gene sequence:

>1143_bases
ATGGAATTTAAGCGGAATATACTTATAACAGGAGGAGCCGGATTTATTGGTAGCCATGTGGTGCGCCTGTTTGTGAACAA
GTATATTGATTGTCATATCATTAACTTGGATAAATTGACATATGCTGGGAATTTGGCAAATCTTAAGGACATAGAGAACA
GGCAAAATTACACTTTTGTAAAGGCTGATATTTGCGATTTTATAAAGATTCAAGAGTTAATGGTGCAATATGAAGTGGAC
GGTGTGATTCATTTAGCAGCAGAAAGCCATGTGGATCGGAGTATCAAGGATCCGTTCACTTTCGCTCGAACGAATGTGAT
GGGAACCCTATCCTTATTACAAGCGGCCAAATTGTATTGGGAATCGCTACCTGAAAAATATGAAGGTAAGATGTTTTACC
ATATCTCAACGGATGAGGTTTATGGTGCGTTAACCATGAGTCATCCGGAGGGAATAGAACCGCCATTTATCACTACCGCT
TCCTCGGGTGGGCATCACCATGCCTACGGAAAGGATTTCTTTTATGAGAATACCAAGTATAACCCACATAGTCCATACTC
CGCAAGTAAGGCGAGCAGTGACCATTTTGTTCGTGCATTCCACGATACCTACGGGATGCCGACTATTGTGACTAATTGTA
GTAATAATTATGGCCCTTACCAGTTCCCAGAGAAGCTGATTCCCTTATTCATTAATAATATTCGTCATCGCAAACCCCTG
CCTGTTTACGGAAGAGGCGAGAATGTGCGGGATTGGTTGTTCGTTGAAGATCATGCCCGTGCCATTGATTTGATATTTCA
TCAGGGTAAGGTTGCGGATACCTATAATATAGGGGGCTTCAATGAATGGAAGAATATTGATATCATCAAGGTGATCATTC
GTACCGTAGACCGTTTGTTGGGACGTAGGGAAGGAGAGGATATGGATCTCATCACTTATGTAACCGACCGTTTGGGACAT
GATGCTCGGTATGCCATTGATTCTACCAAATTGCAGAAAGAACTAGGTTGGGAACCTTTATATCAATTTGAAGAAGGTAT
TGAAAAGACCGTAAAATGGTATTTGGAGAATCAGGAATGGATGGATAACATCACGAGTGGGGAGTGTGAGAGGTATTATG
ATTCTATGTACAACGGTAGGTGA

Upstream 100 bases:

>100_bases
GAAAGCCTTTGTCCCCGAGTTTAAGAGTCAGAATTCTCAGTTTGAGGAGTTGGATTGAATACTTACAAACGTAAAAATAA
TTGACTAAAAATAACGAATG

Downstream 100 bases:

>100_bases
GAAAATATATGGATAACAAGCCGGTAATAATAGAGCTACCCAAGTTTTTAGATAAGCGGGGTAATCTTTCCTTTGTAGAG
GAATTCCAGCATGTTCCATT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MEFKRNILITGGAGFIGSHVVRLFVNKYIDCHIINLDKLTYAGNLANLKDIENRQNYTFVKADICDFIKIQELMVQYEVD
GVIHLAAESHVDRSIKDPFTFARTNVMGTLSLLQAAKLYWESLPEKYEGKMFYHISTDEVYGALTMSHPEGIEPPFITTA
SSGGHHHAYGKDFFYENTKYNPHSPYSASKASSDHFVRAFHDTYGMPTIVTNCSNNYGPYQFPEKLIPLFINNIRHRKPL
PVYGRGENVRDWLFVEDHARAIDLIFHQGKVADTYNIGGFNEWKNIDIIKVIIRTVDRLLGRREGEDMDLITYVTDRLGH
DARYAIDSTKLQKELGWEPLYQFEEGIEKTVKWYLENQEWMDNITSGECERYYDSMYNGR

Sequences:

>Translated_380_residues
MEFKRNILITGGAGFIGSHVVRLFVNKYIDCHIINLDKLTYAGNLANLKDIENRQNYTFVKADICDFIKIQELMVQYEVD
GVIHLAAESHVDRSIKDPFTFARTNVMGTLSLLQAAKLYWESLPEKYEGKMFYHISTDEVYGALTMSHPEGIEPPFITTA
SSGGHHHAYGKDFFYENTKYNPHSPYSASKASSDHFVRAFHDTYGMPTIVTNCSNNYGPYQFPEKLIPLFINNIRHRKPL
PVYGRGENVRDWLFVEDHARAIDLIFHQGKVADTYNIGGFNEWKNIDIIKVIIRTVDRLLGRREGEDMDLITYVTDRLGH
DARYAIDSTKLQKELGWEPLYQFEEGIEKTVKWYLENQEWMDNITSGECERYYDSMYNGR
>Mature_380_residues
MEFKRNILITGGAGFIGSHVVRLFVNKYIDCHIINLDKLTYAGNLANLKDIENRQNYTFVKADICDFIKIQELMVQYEVD
GVIHLAAESHVDRSIKDPFTFARTNVMGTLSLLQAAKLYWESLPEKYEGKMFYHISTDEVYGALTMSHPEGIEPPFITTA
SSGGHHHAYGKDFFYENTKYNPHSPYSASKASSDHFVRAFHDTYGMPTIVTNCSNNYGPYQFPEKLIPLFINNIRHRKPL
PVYGRGENVRDWLFVEDHARAIDLIFHQGKVADTYNIGGFNEWKNIDIIKVIIRTVDRLLGRREGEDMDLITYVTDRLGH
DARYAIDSTKLQKELGWEPLYQFEEGIEKTVKWYLENQEWMDNITSGECERYYDSMYNGR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=355, Percent_Identity=36.9014084507042, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI42516563, Length=362, Percent_Identity=24.0331491712707, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI56237023, Length=373, Percent_Identity=25.4691689008043, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=373, Percent_Identity=25.4691689008043, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=373, Percent_Identity=25.4691689008043, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788353, Length=375, Percent_Identity=52, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI48994969, Length=367, Percent_Identity=50.9536784741144, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1786974, Length=371, Percent_Identity=25.3369272237197, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788366, Length=389, Percent_Identity=24.1645244215938, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=355, Percent_Identity=34.6478873239437, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=353, Percent_Identity=31.1614730878187, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=392, Percent_Identity=25.765306122449, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=394, Percent_Identity=25.6345177664975, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=358, Percent_Identity=23.463687150838, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=366, Percent_Identity=25.9562841530055, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21356223, Length=375, Percent_Identity=23.7333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=385, Percent_Identity=24.4155844155844, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 44008; Mature: 44008

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKRNILITGGAGFIGSHVVRLFVNKYIDCHIINLDKLTYAGNLANLKDIENRQNYTFV
CCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHEEECCCCCCHHHHCCCCCEEEE
KADICDFIKIQELMVQYEVDGVIHLAAESHVDRSIKDPFTFARTNVMGTLSLLQAAKLYW
EHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
ESLPEKYEGKMFYHISTDEVYGALTMSHPEGIEPPFITTASSGGHHHAYGKDFFYENTKY
HHCCHHHCCEEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCC
NPHSPYSASKASSDHFVRAFHDTYGMPTIVTNCSNNYGPYQFPEKLIPLFINNIRHRKPL
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVYGRGENVRDWLFVEDHARAIDLIFHQGKVADTYNIGGFNEWKNIDIIKVIIRTVDRLL
CCCCCCCCCCEEEEECCCHHEEEHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
GRREGEDMDLITYVTDRLGHDARYAIDSTKLQKELGWEPLYQFEEGIEKTVKWYLENQEW
CCCCCCCCHHHHHHHHHHCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHH
MDNITSGECERYYDSMYNGR
HHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MEFKRNILITGGAGFIGSHVVRLFVNKYIDCHIINLDKLTYAGNLANLKDIENRQNYTFV
CCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHEEECCCCCCHHHHCCCCCEEEE
KADICDFIKIQELMVQYEVDGVIHLAAESHVDRSIKDPFTFARTNVMGTLSLLQAAKLYW
EHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
ESLPEKYEGKMFYHISTDEVYGALTMSHPEGIEPPFITTASSGGHHHAYGKDFFYENTKY
HHCCHHHCCEEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCC
NPHSPYSASKASSDHFVRAFHDTYGMPTIVTNCSNNYGPYQFPEKLIPLFINNIRHRKPL
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVYGRGENVRDWLFVEDHARAIDLIFHQGKVADTYNIGGFNEWKNIDIIKVIIRTVDRLL
CCCCCCCCCCEEEEECCCHHEEEHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
GRREGEDMDLITYVTDRLGHDARYAIDSTKLQKELGWEPLYQFEEGIEKTVKWYLENQEW
CCCCCCCCHHHHHHHHHHCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHH
MDNITSGECERYYDSMYNGR
HHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]