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The map label for this gene is ramA [H]

Identifier: 150009902

GI number: 150009902

Start: 4016447

End: 4017406

Strand: Direct

Name: ramA [H]

Synonym: BDI_3319

Alternate gene names: 150009902

Gene position: 4016447-4017406 (Clockwise)

Preceding gene: 150009899

Following gene: 150009903

Centisome position: 83.48

GC content: 49.38

Gene sequence:

>960_bases
ATGGAACCATTAAAAATTGCTACCGTGCAGTTCGAGACACGGGATAACGATAAGGTGTATAATCTTTCAGTCATCCGGGA
GATGTGCCGGAAAGCGTCGGTAGAGGGAGCGGATGTAGTGGCTTTTCACGAATGCTCGGTTTGTGGATATACGTTTGCCA
AGGACTTGTCTCGCGAGGAATTGTTGGCTATTGCCGAACCGATCCCCACGGGGGAGAGTACGCAAGCCTTGATCGCCATC
GCCAAAGAGTTTGGCGTCACGCTACTGGCCGGATTCTTTGAGCGGGATGGGGATCGTATCTATAAAGCGCAAGTTTGCGT
GGATGGCAATGGCTTGAAGGCAAAGTACCGCAAGCTACATCCGTTTATTAATCCGAATATCTTGCCGGGCGATCAATATG
TGGTTTTTGAGATCAAGGGATGGAAATGTGGCATATTGATTTGCTACGATAATAATATCATAGAGAATGTCCGTGCCACG
GCCTTATTAGGGGCGGATATCATTTTCATGCCTCACGTGACAATGTGTACCCCTTCCCCTCGCCCCGGGGCTGGTTTGAT
AGATCCTGTGTTGTGGGAGAACCGGGCAAACGATCCGACTTCCTTGCGACAGGAGTTCGATGGCCTGAAGGGACGTGCTT
GGCTTATGAAATGGCTTCCCGCTAGGGCGTACGACAATGCGGTATATGTGGTATTCTCCAATCCGATCGGGCGTGATTAT
AATGAGATCAAGAACGGTTGCTCGATGATTCTGGATCCTTTCGGCGATATCGTGGCGGAATGCCGTAAGCTGGGGGATGA
TTTCGTGATCGCTACGGCTATCCCCGAGAAATTGAGACAGGCTGGAGGATATCGTTACCGAAATGCCCGTCGTCCGGAAC
TTTATGCGGATATCATCGGTCAACCGCATGAGTCCAATCAAAAGGTAGCATGGTTAACAGAAACAACAAATAACAAATAA

Upstream 100 bases:

>100_bases
TTCATGATCGGTAGAAAAAGTTATCCCGCAAAAGTAGTATATTATTGTTATCTTTGCGAATCTAGTCGAAAAATATATAA
ACCAATTAAATCAAAATATC

Downstream 100 bases:

>100_bases
ATAATATGCAACAAACGACAATCTCTAAGCCGCCTCGTGTAGAGGTGGTGGATGCGCTACGTGGATTCGCTGTCTTGGCC
ATCTTATTGGTGCATCATGT

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MEPLKIATVQFETRDNDKVYNLSVIREMCRKASVEGADVVAFHECSVCGYTFAKDLSREELLAIAEPIPTGESTQALIAI
AKEFGVTLLAGFFERDGDRIYKAQVCVDGNGLKAKYRKLHPFINPNILPGDQYVVFEIKGWKCGILICYDNNIIENVRAT
ALLGADIIFMPHVTMCTPSPRPGAGLIDPVLWENRANDPTSLRQEFDGLKGRAWLMKWLPARAYDNAVYVVFSNPIGRDY
NEIKNGCSMILDPFGDIVAECRKLGDDFVIATAIPEKLRQAGGYRYRNARRPELYADIIGQPHESNQKVAWLTETTNNK

Sequences:

>Translated_319_residues
MEPLKIATVQFETRDNDKVYNLSVIREMCRKASVEGADVVAFHECSVCGYTFAKDLSREELLAIAEPIPTGESTQALIAI
AKEFGVTLLAGFFERDGDRIYKAQVCVDGNGLKAKYRKLHPFINPNILPGDQYVVFEIKGWKCGILICYDNNIIENVRAT
ALLGADIIFMPHVTMCTPSPRPGAGLIDPVLWENRANDPTSLRQEFDGLKGRAWLMKWLPARAYDNAVYVVFSNPIGRDY
NEIKNGCSMILDPFGDIVAECRKLGDDFVIATAIPEKLRQAGGYRYRNARRPELYADIIGQPHESNQKVAWLTETTNNK
>Mature_319_residues
MEPLKIATVQFETRDNDKVYNLSVIREMCRKASVEGADVVAFHECSVCGYTFAKDLSREELLAIAEPIPTGESTQALIAI
AKEFGVTLLAGFFERDGDRIYKAQVCVDGNGLKAKYRKLHPFINPNILPGDQYVVFEIKGWKCGILICYDNNIIENVRAT
ALLGADIIFMPHVTMCTPSPRPGAGLIDPVLWENRANDPTSLRQEFDGLKGRAWLMKWLPARAYDNAVYVVFSNPIGRDY
NEIKNGCSMILDPFGDIVAECRKLGDDFVIATAIPEKLRQAGGYRYRNARRPELYADIIGQPHESNQKVAWLTETTNNK

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=26.6423357664234, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 35755; Mature: 35755

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLKIATVQFETRDNDKVYNLSVIREMCRKASVEGADVVAFHECSVCGYTFAKDLSREE
CCCEEEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCHHH
LLAIAEPIPTGESTQALIAIAKEFGVTLLAGFFERDGDRIYKAQVCVDGNGLKAKYRKLH
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHC
PFINPNILPGDQYVVFEIKGWKCGILICYDNNIIENVRATALLGADIIFMPHVTMCTPSP
CCCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCEEEECCEEEECCCC
RPGAGLIDPVLWENRANDPTSLRQEFDGLKGRAWLMKWLPARAYDNAVYVVFSNPIGRDY
CCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCH
NEIKNGCSMILDPFGDIVAECRKLGDDFVIATAIPEKLRQAGGYRYRNARRPELYADIIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHCC
QPHESNQKVAWLTETTNNK
CCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MEPLKIATVQFETRDNDKVYNLSVIREMCRKASVEGADVVAFHECSVCGYTFAKDLSREE
CCCEEEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCHHH
LLAIAEPIPTGESTQALIAIAKEFGVTLLAGFFERDGDRIYKAQVCVDGNGLKAKYRKLH
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHC
PFINPNILPGDQYVVFEIKGWKCGILICYDNNIIENVRATALLGADIIFMPHVTMCTPSP
CCCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCEEEECCEEEECCCC
RPGAGLIDPVLWENRANDPTSLRQEFDGLKGRAWLMKWLPARAYDNAVYVVFSNPIGRDY
CCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCH
NEIKNGCSMILDPFGDIVAECRKLGDDFVIATAIPEKLRQAGGYRYRNARRPELYADIIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHCC
QPHESNQKVAWLTETTNNK
CCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA