Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 150009886

GI number: 150009886

Start: 3995607

End: 3997064

Strand: Direct

Name: pyk [H]

Synonym: BDI_3302

Alternate gene names: 150009886

Gene position: 3995607-3997064 (Clockwise)

Preceding gene: 150009885

Following gene: 150009887

Centisome position: 83.04

GC content: 47.94

Gene sequence:

>1458_bases
ATGTTAAAGCATACGAAGATTGTTGCTACAGTTTCCGACCAGCGCTGCGACGTTGCGTTCGTTGAGGCGTTATATAAAGC
GGGAATGAATGTGGTGCGCCTGAATACGGCTCACATGATGGAAGAGGGGTTGACCCGTGTGGTAAATAACGTCCGTACAG
TATCCGACCGTATCGGTATCTTGATGGATACAAAGGGGCCGGAAGTACGTACCACGACGACGGTAAACAAGGAACCGATC
CCTTTCAAGACTGGAGAGATCGTGAAGGTAATCGGTAATCCCGATCAGGAAACTTCGCATGATTGTATTTGTGTGTCATA
TAAGAATTTCGTGAACGACTTGGCGATTGGCAGCGACATCCTTATCGATGATGGTGATCTGGAAATGAAGGTTACCGGGA
AATCCGGCGATTGTTTGCTTTGCGAGATCCAGAATGACGCTACGCTAGGTAGCCGCAAGAGCGTGAATGTGCCGGGCGTA
CGTATCAATTTGCCTTCGTTGACTGAGAAAGACCGTAATAATATCCTTTGGGCGATCGATCATGATCTCGATTTTATCGC
TCACTCATTTGTTCGCAACAAACAGGACGTATTGGATATCCAACGTATCTTGGACGAGCGTAACAGCCCTATCAAGATTA
TCGCTAAGATCGAGAATCAAGAGGGTGTAGACAATATCGAGGAGATTTTGGAAGTGGCTTATGGCGTTATGATCGCTCGT
GGTGACTTGGGTATCGAGGTTCCGGCCGAGAAGATCCCGGGTATCCAGCGTATGCTGATCCGTAAATGCGTGGAGGTGAA
GAAGCCGGTTATCGTGGCTACGCAAATGCTTCATTCCATGATTAATAATCCTCGTCCTACTCGTGCCGAGGTTACGGATA
TCGCCAACGCTATTTATTATCGTACGGATGCTTTGATGTTAAGCGGTGAGACGGCTTATGGAAAATATCCGATCGAGGCG
GTACAGACCATGACGAAAGTGGCTCGTGAAGCCGAGAAGACCAAGCTTTCCGCGAATGATATCCGTGTGCCGATCGAGGG
GAATGATTTAGACGTAACTTCTTTCTTGGCGAAACAAGCTGTTAAGTCTTCTTCTAAATTACATGTGAAGGCGATTATCA
CGGATAGTTACACGGGTCGTACGGCACGTTATCTGGCCGCTTTCCGCGGAACCTCTACTGTGTTCGCTATATGCTATAAT
CCTCGTGTGATGCGTATGTTGGGTCTTTCTTATGGGGTATGGGCGGTTCATCAGCCGTACAACGACAGCCGTAGGGGATA
TTTCTATGATGCGTTGAACCAGCTGATCCATAGCGGACGTATCACTCGCAACGATATGGTAGCGTATTTGAGCGGTAGCT
TCGGCGAAGGTGGCGGTACCAGCTTTTTGGAGATTAACAACGTAGGAAAGGTGCTGGATGCCGGCACGCAATATCAGTTG
CCTACTTTTAAAGAGTAA

Upstream 100 bases:

>100_bases
GCTGGATTCGTATCGGTTGGCGATCGAATCGTTTATGTGATAGAAGGTTTAGAAGGTTATCAAATAGAATTAATAATTAG
GTTATAATAATTTAAGGTAT

Downstream 100 bases:

>100_bases
GAGGAATGTTTACGGGCGAGATTGATGCGTATATTCTTTCTCATATAGATGAAGAAGGGGATTTGCTGTCGGCGTTGAAT
CGCGACGCCAATGTGAACCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLKHTKIVATVSDQRCDVAFVEALYKAGMNVVRLNTAHMMEEGLTRVVNNVRTVSDRIGILMDTKGPEVRTTTTVNKEPI
PFKTGEIVKVIGNPDQETSHDCICVSYKNFVNDLAIGSDILIDDGDLEMKVTGKSGDCLLCEIQNDATLGSRKSVNVPGV
RINLPSLTEKDRNNILWAIDHDLDFIAHSFVRNKQDVLDIQRILDERNSPIKIIAKIENQEGVDNIEEILEVAYGVMIAR
GDLGIEVPAEKIPGIQRMLIRKCVEVKKPVIVATQMLHSMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPIEA
VQTMTKVAREAEKTKLSANDIRVPIEGNDLDVTSFLAKQAVKSSSKLHVKAIITDSYTGRTARYLAAFRGTSTVFAICYN
PRVMRMLGLSYGVWAVHQPYNDSRRGYFYDALNQLIHSGRITRNDMVAYLSGSFGEGGGTSFLEINNVGKVLDAGTQYQL
PTFKE

Sequences:

>Translated_485_residues
MLKHTKIVATVSDQRCDVAFVEALYKAGMNVVRLNTAHMMEEGLTRVVNNVRTVSDRIGILMDTKGPEVRTTTTVNKEPI
PFKTGEIVKVIGNPDQETSHDCICVSYKNFVNDLAIGSDILIDDGDLEMKVTGKSGDCLLCEIQNDATLGSRKSVNVPGV
RINLPSLTEKDRNNILWAIDHDLDFIAHSFVRNKQDVLDIQRILDERNSPIKIIAKIENQEGVDNIEEILEVAYGVMIAR
GDLGIEVPAEKIPGIQRMLIRKCVEVKKPVIVATQMLHSMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPIEA
VQTMTKVAREAEKTKLSANDIRVPIEGNDLDVTSFLAKQAVKSSSKLHVKAIITDSYTGRTARYLAAFRGTSTVFAICYN
PRVMRMLGLSYGVWAVHQPYNDSRRGYFYDALNQLIHSGRITRNDMVAYLSGSFGEGGGTSFLEINNVGKVLDAGTQYQL
PTFKE
>Mature_485_residues
MLKHTKIVATVSDQRCDVAFVEALYKAGMNVVRLNTAHMMEEGLTRVVNNVRTVSDRIGILMDTKGPEVRTTTTVNKEPI
PFKTGEIVKVIGNPDQETSHDCICVSYKNFVNDLAIGSDILIDDGDLEMKVTGKSGDCLLCEIQNDATLGSRKSVNVPGV
RINLPSLTEKDRNNILWAIDHDLDFIAHSFVRNKQDVLDIQRILDERNSPIKIIAKIENQEGVDNIEEILEVAYGVMIAR
GDLGIEVPAEKIPGIQRMLIRKCVEVKKPVIVATQMLHSMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPIEA
VQTMTKVAREAEKTKLSANDIRVPIEGNDLDVTSFLAKQAVKSSSKLHVKAIITDSYTGRTARYLAAFRGTSTVFAICYN
PRVMRMLGLSYGVWAVHQPYNDSRRGYFYDALNQLIHSGRITRNDMVAYLSGSFGEGGGTSFLEINNVGKVLDAGTQYQL
PTFKE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=432, Percent_Identity=38.4259259259259, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI33286420, Length=432, Percent_Identity=38.4259259259259, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI33286418, Length=433, Percent_Identity=38.568129330254, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI10835121, Length=456, Percent_Identity=36.6228070175439, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI32967597, Length=456, Percent_Identity=36.6228070175439, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.6306620209059, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.6306620209059, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.0425531914894, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.0425531914894, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.0467289719626, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=37.2340425531915, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788160, Length=468, Percent_Identity=33.1196581196581, Blast_Score=220, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17544584, Length=428, Percent_Identity=37.8504672897196, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=36.6255144032922, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=36.6255144032922, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=36.6255144032922, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=36.6255144032922, Blast_Score=276, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=35.6701030927835, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=35.3061224489796, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24648964, Length=427, Percent_Identity=37.9391100702576, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI28571814, Length=427, Percent_Identity=37.9391100702576, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24648966, Length=319, Percent_Identity=39.4984326018809, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=32.1678321678322, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24646914, Length=267, Percent_Identity=33.7078651685393, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53852; Mature: 53852

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHTKIVATVSDQRCDVAFVEALYKAGMNVVRLNTAHMMEEGLTRVVNNVRTVSDRIGI
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCE
LMDTKGPEVRTTTTVNKEPIPFKTGEIVKVIGNPDQETSHDCICVSYKNFVNDLAIGSDI
EEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCEE
LIDDGDLEMKVTGKSGDCLLCEIQNDATLGSRKSVNVPGVRINLPSLTEKDRNNILWAID
EEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEE
HDLDFIAHSFVRNKQDVLDIQRILDERNSPIKIIAKIENQEGVDNIEEILEVAYGVMIAR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEE
GDLGIEVPAEKIPGIQRMLIRKCVEVKKPVIVATQMLHSMINNPRPTRAEVTDIANAIYY
CCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHE
RTDALMLSGETAYGKYPIEAVQTMTKVAREAEKTKLSANDIRVPIEGNDLDVTSFLAKQA
EECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
VKSSSKLHVKAIITDSYTGRTARYLAAFRGTSTVFAICYNPRVMRMLGLSYGVWAVHQPY
HCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHCCCCCEEEEECCC
NDSRRGYFYDALNQLIHSGRITRNDMVAYLSGSFGEGGGTSFLEINNVGKVLDAGTQYQL
CCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHCCCCCCCC
PTFKE
CCCCC
>Mature Secondary Structure
MLKHTKIVATVSDQRCDVAFVEALYKAGMNVVRLNTAHMMEEGLTRVVNNVRTVSDRIGI
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCE
LMDTKGPEVRTTTTVNKEPIPFKTGEIVKVIGNPDQETSHDCICVSYKNFVNDLAIGSDI
EEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCEE
LIDDGDLEMKVTGKSGDCLLCEIQNDATLGSRKSVNVPGVRINLPSLTEKDRNNILWAID
EEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEE
HDLDFIAHSFVRNKQDVLDIQRILDERNSPIKIIAKIENQEGVDNIEEILEVAYGVMIAR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEE
GDLGIEVPAEKIPGIQRMLIRKCVEVKKPVIVATQMLHSMINNPRPTRAEVTDIANAIYY
CCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHE
RTDALMLSGETAYGKYPIEAVQTMTKVAREAEKTKLSANDIRVPIEGNDLDVTSFLAKQA
EECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
VKSSSKLHVKAIITDSYTGRTARYLAAFRGTSTVFAICYNPRVMRMLGLSYGVWAVHQPY
HCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHCCCCCEEEEECCC
NDSRRGYFYDALNQLIHSGRITRNDMVAYLSGSFGEGGGTSFLEINNVGKVLDAGTQYQL
CCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHCCCCCCCC
PTFKE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]