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The map label for this gene is ecoRIM [H]

Identifier: 150009838

GI number: 150009838

Start: 3936402

End: 3937367

Strand: Direct

Name: ecoRIM [H]

Synonym: BDI_3254

Alternate gene names: 150009838

Gene position: 3936402-3937367 (Clockwise)

Preceding gene: 150009837

Following gene: 150009839

Centisome position: 81.81

GC content: 31.06

Gene sequence:

>966_bases
ATGGCGAGAAATGCGACAAATGGTTTATTACAAAAGGCTAAGAAATCTAAAAGTGATGAGTTCTATACGCAACTTTGTGA
TATAGAACGGGAACTCCAGTATTACCAAGGTCAATTTGAAGATAAAATTGTATATTGTAATTGCGACGACCCTAGGGTTA
GTAATTTTTTTAAATATTTTGCAGCAAATTTCCATAACCTAGGATTAAAGAAACTAATTGCATCCTGTTACATAGAATCC
GACAATAGTATATTTAATGAACAGAATTTTAAAACAGGATTCTATTGTGAGTATTTTGGCAAGAGTAATCAAAATATTGA
GATTATAAATTTTAAAGGTAATGGTGACTTTAGAAGTGCTGAAAGTATTGAATTACTAAAACAATCTGATATTGTTGTCA
CTAATCCACCATTTTCATTATTCAGAGAATTTGTCTCTCAAATAATTAAATACAATAAAAAGTTTTTAGTAATTGGAAAT
GTTAATGCCATTACATATAAAGATATATTTGAGTTAATCCAAAGGAATAAAGCTTGGTTAGGTGTAAATCTAGGCAGAGG
GATTTCCGGTTTTATTGTTCCTGACCATTATGAGCGTTATGGCACAGAAGTAAGTGTTAATAGTGATGGACAAACAATAA
TTGCAACTAACGGTTGTCTGTGGTTGACAAATTTAGAATTGTCACAACGCCATAAAGATATTGTATTGACAAAAGAATAT
AAAGGAAATGAGGACAAGTATCCACATTTTGATAATTGCGATGGTATAAATGTTAATAGAACGCAAGATATACCTAAGGA
TTATCATGGCTTAATGGGGGTGCCTATAACTTTTCTACACAAATATAATCCTGAACAGTTCAAAATTGTAAGATTTCGAA
AAGGGGATGATGGCAAAGATTTGCGGATAAATGGCAAATGTCCTTATTTCAGAATACTCGTTAAAAACAAAAAGATTGAT
GCATAA

Upstream 100 bases:

>100_bases
TTGATATAATGCTTGAAATATCAAAAACATCCTTACAAATATTGGGTAGTGATTTATTCAAGCAATTAACAAAGAAATAG
ACATTTCAGAGGAGTTTGTT

Downstream 100 bases:

>100_bases
GGTTAAGACATTATTAGATTTATGAAGATTAAAGATATAGAGGATATAAATTTTCTTATTGATAATTCTGTAGAAGAATC
TACTGAATTAGAATATAAAC

Product: EcoRI methylase/methyltransferase

Products: NA

Alternate protein names: M.EcoRI; Adenine-specific methyltransferase EcoRI [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MARNATNGLLQKAKKSKSDEFYTQLCDIERELQYYQGQFEDKIVYCNCDDPRVSNFFKYFAANFHNLGLKKLIASCYIES
DNSIFNEQNFKTGFYCEYFGKSNQNIEIINFKGNGDFRSAESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGN
VNAITYKDIFELIQRNKAWLGVNLGRGISGFIVPDHYERYGTEVSVNSDGQTIIATNGCLWLTNLELSQRHKDIVLTKEY
KGNEDKYPHFDNCDGINVNRTQDIPKDYHGLMGVPITFLHKYNPEQFKIVRFRKGDDGKDLRINGKCPYFRILVKNKKID
A

Sequences:

>Translated_321_residues
MARNATNGLLQKAKKSKSDEFYTQLCDIERELQYYQGQFEDKIVYCNCDDPRVSNFFKYFAANFHNLGLKKLIASCYIES
DNSIFNEQNFKTGFYCEYFGKSNQNIEIINFKGNGDFRSAESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGN
VNAITYKDIFELIQRNKAWLGVNLGRGISGFIVPDHYERYGTEVSVNSDGQTIIATNGCLWLTNLELSQRHKDIVLTKEY
KGNEDKYPHFDNCDGINVNRTQDIPKDYHGLMGVPITFLHKYNPEQFKIVRFRKGDDGKDLRINGKCPYFRILVKNKKID
A
>Mature_320_residues
ARNATNGLLQKAKKSKSDEFYTQLCDIERELQYYQGQFEDKIVYCNCDDPRVSNFFKYFAANFHNLGLKKLIASCYIESD
NSIFNEQNFKTGFYCEYFGKSNQNIEIINFKGNGDFRSAESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGNV
NAITYKDIFELIQRNKAWLGVNLGRGISGFIVPDHYERYGTEVSVNSDGQTIIATNGCLWLTNLELSQRHKDIVLTKEYK
GNEDKYPHFDNCDGINVNRTQDIPKDYHGLMGVPITFLHKYNPEQFKIVRFRKGDDGKDLRINGKCPYFRILVKNKKIDA

Specific function: This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052 [H]

Pfam domain/function: NA

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 37211; Mature: 37079

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNATNGLLQKAKKSKSDEFYTQLCDIERELQYYQGQFEDKIVYCNCDDPRVSNFFKYF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
AANFHNLGLKKLIASCYIESDNSIFNEQNFKTGFYCEYFGKSNQNIEIINFKGNGDFRSA
HHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
ESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGNVNAITYKDIFELIQRNKAWL
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHCCCCEE
GVNLGRGISGFIVPDHYERYGTEVSVNSDGQTIIATNGCLWLTNLELSQRHKDIVLTKEY
EEECCCCCCEEECCCHHHHCCCEEEECCCCCEEEEECCEEEEEECCHHCCCCCEEEEECC
KGNEDKYPHFDNCDGINVNRTQDIPKDYHGLMGVPITFLHKYNPEQFKIVRFRKGDDGKD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCE
LRINGKCPYFRILVKNKKIDA
EEECCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
ARNATNGLLQKAKKSKSDEFYTQLCDIERELQYYQGQFEDKIVYCNCDDPRVSNFFKYF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
AANFHNLGLKKLIASCYIESDNSIFNEQNFKTGFYCEYFGKSNQNIEIINFKGNGDFRSA
HHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
ESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGNVNAITYKDIFELIQRNKAWL
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHCCCCEE
GVNLGRGISGFIVPDHYERYGTEVSVNSDGQTIIATNGCLWLTNLELSQRHKDIVLTKEY
EEECCCCCCEEECCCHHHHCCCEEEECCCCCEEEEECCEEEEEECCHHCCCCCEEEEECC
KGNEDKYPHFDNCDGINVNRTQDIPKDYHGLMGVPITFLHKYNPEQFKIVRFRKGDDGKD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCE
LRINGKCPYFRILVKNKKIDA
EEECCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6257703; 6257701; 6257702 [H]