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The map label for this gene is ecoRIR [H]

Identifier: 150009837

GI number: 150009837

Start: 3935548

End: 3936381

Strand: Direct

Name: ecoRIR [H]

Synonym: BDI_3253

Alternate gene names: 150009837

Gene position: 3935548-3936381 (Clockwise)

Preceding gene: 150009836

Following gene: 150009838

Centisome position: 81.8

GC content: 34.17

Gene sequence:

>834_bases
ATGGCAAAGAAAAATCAATCAACAAGATTAACTCTTCAGCACAAAGAAAGTCATGGCGTTGAAGGTATCTTCGGCGAAGA
AGCCAAATTGCATGATTTGACAGTTGGGGAAATTTCTCATCATGTCATTGCTAAACTAACAGAAGATTATCCTCAGTTGA
CATTTCGATATAGACAAAGTATAGAAAAAAAAGAAATCAATGAGGCATTACAAAAGGTTGACCCAAATCTTGGACAAACC
TTATTTGTAGAAAATGCAAGCATCATTCCTGATGGTGGAATAATAGAGGTAAAAGACGATAACGAAAATTGGAGAGTTGT
ATTAGTGTCAGAAGCTAAACATCAGGGAAAGGATATAGAAAATATTAGAAAAGGTGAGCTGGTCGGAAAGAATAAAGACC
AAGATTTAATGGCAGCTGGAAATGCGATAGAACGTTCTCACAAGAATATTTCCGAAATTGCCAACTTTATGTTAGCGGAA
TCTCATTTCCCATATGTTTTGTTTCTTGAAGGTTCTAATTTTTTGACTGAGACTATAAAAGTAACAAGACCTGACGGACG
AGTAGTTACTCTTGAATATAATTCAGGGATGCTTAACAGATTAGACAGATTAACGGCTGCAAATTATGGAATGCCTATCA
ATACCAATCTTTGTAAGAATATGTTTGTCAAGCATAAGGATAAAACAATAATGCTCCAAGCGACATCTATTTATACTCAA
GGAGATGGAGGTAGTTGGAATAAAAGTAAGATGTTTGATATAATGCTTGAAATATCAAAAACATCCTTACAAATATTGGG
TAGTGATTTATTCAAGCAATTAACAAAGAAATAG

Upstream 100 bases:

>100_bases
CCATACGAAATTTGCGAAAATATAGAAATAGAGAAATTTCTTGAAAAAGTAAACAAAGAGGAAACCTACACTAAATAGAA
TAATATTTAACAATAATAAT

Downstream 100 bases:

>100_bases
ACATTTCAGAGGAGTTTGTTATGGCGAGAAATGCGACAAATGGTTTATTACAAAAGGCTAAGAAATCTAAAAGTGATGAG
TTCTATACGCAACTTTGTGA

Product: Type II restriction enzyme EcoRI

Products: NA

Alternate protein names: R.EcoRI; Endonuclease EcoRI; Type II restriction enzyme EcoRI [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MAKKNQSTRLTLQHKESHGVEGIFGEEAKLHDLTVGEISHHVIAKLTEDYPQLTFRYRQSIEKKEINEALQKVDPNLGQT
LFVENASIIPDGGIIEVKDDNENWRVVLVSEAKHQGKDIENIRKGELVGKNKDQDLMAAGNAIERSHKNISEIANFMLAE
SHFPYVLFLEGSNFLTETIKVTRPDGRVVTLEYNSGMLNRLDRLTAANYGMPINTNLCKNMFVKHKDKTIMLQATSIYTQ
GDGGSWNKSKMFDIMLEISKTSLQILGSDLFKQLTKK

Sequences:

>Translated_277_residues
MAKKNQSTRLTLQHKESHGVEGIFGEEAKLHDLTVGEISHHVIAKLTEDYPQLTFRYRQSIEKKEINEALQKVDPNLGQT
LFVENASIIPDGGIIEVKDDNENWRVVLVSEAKHQGKDIENIRKGELVGKNKDQDLMAAGNAIERSHKNISEIANFMLAE
SHFPYVLFLEGSNFLTETIKVTRPDGRVVTLEYNSGMLNRLDRLTAANYGMPINTNLCKNMFVKHKDKTIMLQATSIYTQ
GDGGSWNKSKMFDIMLEISKTSLQILGSDLFKQLTKK
>Mature_276_residues
AKKNQSTRLTLQHKESHGVEGIFGEEAKLHDLTVGEISHHVIAKLTEDYPQLTFRYRQSIEKKEINEALQKVDPNLGQTL
FVENASIIPDGGIIEVKDDNENWRVVLVSEAKHQGKDIENIRKGELVGKNKDQDLMAAGNAIERSHKNISEIANFMLAES
HFPYVLFLEGSNFLTETIKVTRPDGRVVTLEYNSGMLNRLDRLTAANYGMPINTNLCKNMFVKHKDKTIMLQATSIYTQG
DGGSWNKSKMFDIMLEISKTSLQILGSDLFKQLTKK

Specific function: Recognizes the double-stranded sequence GAATTC and cleaves after G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR004221
- InterPro:   IPR011336
- InterPro:   IPR018131
- ProDom:   PD039825 [H]

Pfam domain/function: PF02963 EcoRI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 31306; Mature: 31175

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNQSTRLTLQHKESHGVEGIFGEEAKLHDLTVGEISHHVIAKLTEDYPQLTFRYRQS
CCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKKEINEALQKVDPNLGQTLFVENASIIPDGGIIEVKDDNENWRVVLVSEAKHQGKDIE
HHHHHHHHHHHHHCCCCCCEEEEECCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCHH
NIRKGELVGKNKDQDLMAAGNAIERSHKNISEIANFMLAESHFPYVLFLEGSNFLTETIK
HHHCCCCCCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEE
VTRPDGRVVTLEYNSGMLNRLDRLTAANYGMPINTNLCKNMFVKHKDKTIMLQATSIYTQ
EECCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEC
GDGGSWNKSKMFDIMLEISKTSLQILGSDLFKQLTKK
CCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKNQSTRLTLQHKESHGVEGIFGEEAKLHDLTVGEISHHVIAKLTEDYPQLTFRYRQS
CCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKKEINEALQKVDPNLGQTLFVENASIIPDGGIIEVKDDNENWRVVLVSEAKHQGKDIE
HHHHHHHHHHHHHCCCCCCEEEEECCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCHH
NIRKGELVGKNKDQDLMAAGNAIERSHKNISEIANFMLAESHFPYVLFLEGSNFLTETIK
HHHCCCCCCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEE
VTRPDGRVVTLEYNSGMLNRLDRLTAANYGMPINTNLCKNMFVKHKDKTIMLQATSIYTQ
EECCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEC
GDGGSWNKSKMFDIMLEISKTSLQILGSDLFKQLTKK
CCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6257703; 6257701; 6257702; 3024321; 2399465; 2254311 [H]