Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 150009765

GI number: 150009765

Start: 3846319

End: 3847467

Strand: Reverse

Name: phoA [H]

Synonym: BDI_3180

Alternate gene names: 150009765

Gene position: 3847467-3846319 (Counterclockwise)

Preceding gene: 150009766

Following gene: 150009764

Centisome position: 79.97

GC content: 54.31

Gene sequence:

>1149_bases
ATGAGAAGAACAACTCTATCCGTGCTATCCGCCTTAGCCATCCTGCTATCCGGCATGATCGTTCCGGAGGCCGCGGCACA
AAGTCGCCGAGACAAAGAACAAACGTATGTACTAGAGAAACCTTACGAGGTCAAGAAACTAGTCCCCCCTACCGGAAAGA
AAATAAAGAACGTGATCCTAATGATCGGCGACGGTATGAGCCTTATGCACATGTATTCCGCTTGGACCGCCAACCGGGGC
AAGCTCTGGCTGGACAACTCCCAATACACCGGCCTCTCGAAAACCTATTGCGCCAACCGCCTCATCACCGACTCCGGAGC
CGGAGGTACCGCACTGGCCACCGGCCATAAAACGAACTACCATATGGTAGGCGTAGACCCGGAAGGCAAGCCCTTGGAAT
CTTTGGCTACCTTGGCGAATAAGAAAGGCCTGTCAAGCGGCATCGCCGTTACCTGTCGCCTATGGGATGCTACGCCCGCC
GACTTCTGCTGCCACAATACGGATCGTGACGCCGAGGCCGAGATCGTAGCGGATTACGTGAACTGTGGCGTCGATTACGT
ATTCGGCGGCGGCTCCAAGCTATTCGAGAACCGTGCGGACGGTCGCGATTTGTTCAAGGAATTAAGGGAGAAAGGTTACC
AGACTCCCCGTAGCTGGGAGGAACTGGCGAAGATCCAAAAAGGCAAGGTATTCTGCGTTACCGATACCGTAGACACCCCG
CTTCCCGCCGAGCGAGGCGATCTCTTGGCTCGTGCCTCGATGAAAGCGATCGATCTGCTGGGACAAAACCCGGAAGGTTT
CTTCCTGATGGTAGAGGGCTCCCAATTGGATGATTACGGACATTTCAACGATATCGACCTGTTGATGCAAGAGACACATG
ATTTCGACCGTACGATCGGACGTATCTTCGAATGGGCCGCCCAAGACGGTGAGACATTGGTCGTCGTAACCGCCGATCAC
GAGACCGGTGGCCTTACCTTGGTGGACGGGGATCTGAACGAGGGCCGTATCGTTTGTAAATTCTCCACCGGAGGGCATAG
CGGCGTAATGGTCCCCGTTTATGCTTTCGGCCCGGGCGCCGAGAACTTCACGGGTATCTTCGAGAATACGGATATCTTCT
GGAAGATCAAAAAGCTCCTGAATCTATAA

Upstream 100 bases:

>100_bases
GTACCTAGGCTGGAGCTGGCGTACGCCAAGCATGTCCAAACTAGACGCGATCCCAACAGAGAACCTTTATATAAATTGAT
AATATTAAATACTGAATCAT

Downstream 100 bases:

>100_bases
TCAAACAGTATTCATCCGATGGAGAGTATAACAGGGCTATCCCGCTAGTCAAGTACCGCAGTACTTTGGCTGAGAGGACA
GCGGTACTTGACTAGCAGGA

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRRTTLSVLSALAILLSGMIVPEAAAQSRRDKEQTYVLEKPYEVKKLVPPTGKKIKNVILMIGDGMSLMHMYSAWTANRG
KLWLDNSQYTGLSKTYCANRLITDSGAGGTALATGHKTNYHMVGVDPEGKPLESLATLANKKGLSSGIAVTCRLWDATPA
DFCCHNTDRDAEAEIVADYVNCGVDYVFGGGSKLFENRADGRDLFKELREKGYQTPRSWEELAKIQKGKVFCVTDTVDTP
LPAERGDLLARASMKAIDLLGQNPEGFFLMVEGSQLDDYGHFNDIDLLMQETHDFDRTIGRIFEWAAQDGETLVVVTADH
ETGGLTLVDGDLNEGRIVCKFSTGGHSGVMVPVYAFGPGAENFTGIFENTDIFWKIKKLLNL

Sequences:

>Translated_382_residues
MRRTTLSVLSALAILLSGMIVPEAAAQSRRDKEQTYVLEKPYEVKKLVPPTGKKIKNVILMIGDGMSLMHMYSAWTANRG
KLWLDNSQYTGLSKTYCANRLITDSGAGGTALATGHKTNYHMVGVDPEGKPLESLATLANKKGLSSGIAVTCRLWDATPA
DFCCHNTDRDAEAEIVADYVNCGVDYVFGGGSKLFENRADGRDLFKELREKGYQTPRSWEELAKIQKGKVFCVTDTVDTP
LPAERGDLLARASMKAIDLLGQNPEGFFLMVEGSQLDDYGHFNDIDLLMQETHDFDRTIGRIFEWAAQDGETLVVVTADH
ETGGLTLVDGDLNEGRIVCKFSTGGHSGVMVPVYAFGPGAENFTGIFENTDIFWKIKKLLNL
>Mature_382_residues
MRRTTLSVLSALAILLSGMIVPEAAAQSRRDKEQTYVLEKPYEVKKLVPPTGKKIKNVILMIGDGMSLMHMYSAWTANRG
KLWLDNSQYTGLSKTYCANRLITDSGAGGTALATGHKTNYHMVGVDPEGKPLESLATLANKKGLSSGIAVTCRLWDATPA
DFCCHNTDRDAEAEIVADYVNCGVDYVFGGGSKLFENRADGRDLFKELREKGYQTPRSWEELAKIQKGKVFCVTDTVDTP
LPAERGDLLARASMKAIDLLGQNPEGFFLMVEGSQLDDYGHFNDIDLLMQETHDFDRTIGRIFEWAAQDGETLVVVTADH
ETGGLTLVDGDLNEGRIVCKFSTGGHSGVMVPVYAFGPGAENFTGIFENTDIFWKIKKLLNL

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=376, Percent_Identity=27.9255319148936, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI94721246, Length=336, Percent_Identity=27.6785714285714, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI157266296, Length=336, Percent_Identity=27.6785714285714, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157266292, Length=355, Percent_Identity=28.169014084507, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI48994877, Length=416, Percent_Identity=29.0865384615385, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=316, Percent_Identity=30.6962025316456, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355981, Length=375, Percent_Identity=28.5333333333333, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19921912, Length=459, Percent_Identity=27.4509803921569, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358067, Length=460, Percent_Identity=25.2173913043478, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI18859923, Length=293, Percent_Identity=29.0102389078498, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355149, Length=353, Percent_Identity=27.1954674220963, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355151, Length=329, Percent_Identity=26.7477203647416, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI85725200, Length=331, Percent_Identity=26.2839879154079, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI85815833, Length=331, Percent_Identity=26.2839879154079, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24657827, Length=376, Percent_Identity=26.5957446808511, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651554, Length=364, Percent_Identity=27.1978021978022, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585213, Length=340, Percent_Identity=25, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24657835, Length=338, Percent_Identity=25.4437869822485, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24657842, Length=338, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 41965; Mature: 41965

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTTLSVLSALAILLSGMIVPEAAAQSRRDKEQTYVLEKPYEVKKLVPPTGKKIKNVIL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHEEEECCCCHHHHCCCCCCCCCEEEEE
MIGDGMSLMHMYSAWTANRGKLWLDNSQYTGLSKTYCANRLITDSGAGGTALATGHKTNY
EECCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCEEECCCCCCCEEECCCCCCE
HMVGVDPEGKPLESLATLANKKGLSSGIAVTCRLWDATPADFCCHNTDRDAEAEIVADYV
EEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCHHHHHHHHH
NCGVDYVFGGGSKLFENRADGRDLFKELREKGYQTPRSWEELAKIQKGKVFCVTDTVDTP
HCCEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCC
LPAERGDLLARASMKAIDLLGQNPEGFFLMVEGSQLDDYGHFNDIDLLMQETHDFDRTIG
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RIFEWAAQDGETLVVVTADHETGGLTLVDGDLNEGRIVCKFSTGGHSGVMVPVYAFGPGA
HHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCCC
ENFTGIFENTDIFWKIKKLLNL
CCCCEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MRRTTLSVLSALAILLSGMIVPEAAAQSRRDKEQTYVLEKPYEVKKLVPPTGKKIKNVIL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHEEEECCCCHHHHCCCCCCCCCEEEEE
MIGDGMSLMHMYSAWTANRGKLWLDNSQYTGLSKTYCANRLITDSGAGGTALATGHKTNY
EECCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCEEECCCCCCCEEECCCCCCE
HMVGVDPEGKPLESLATLANKKGLSSGIAVTCRLWDATPADFCCHNTDRDAEAEIVADYV
EEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCHHHHHHHHH
NCGVDYVFGGGSKLFENRADGRDLFKELREKGYQTPRSWEELAKIQKGKVFCVTDTVDTP
HCCEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCC
LPAERGDLLARASMKAIDLLGQNPEGFFLMVEGSQLDDYGHFNDIDLLMQETHDFDRTIG
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RIFEWAAQDGETLVVVTADHETGGLTLVDGDLNEGRIVCKFSTGGHSGVMVPVYAFGPGA
HHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCCC
ENFTGIFENTDIFWKIKKLLNL
CCCCEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]