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The map label for this gene is tyrS

Identifier: 150009644

GI number: 150009644

Start: 3660030

End: 3661322

Strand: Direct

Name: tyrS

Synonym: BDI_3058

Alternate gene names: 150009644

Gene position: 3660030-3661322 (Clockwise)

Preceding gene: 150009643

Following gene: 150009645

Centisome position: 76.07

GC content: 45.32

Gene sequence:

>1293_bases
ATGAATTTTGTAGAAGAATTAAGATGGAGGGGCATGATCGCTGATATGATGCCCGGGACGGAAGAGCAATTGCAGAAAGA
GTTGACATCGGCCTATGTGGGGATTGACCCAACGGCAGACTCTTTGCATATCGGACACTTAGTAAGTGTGATGATGCTGA
AGCATTTCCAACGTGCCGGTCATCGTCCTATCGCTTTAGTGGGTGGCGCTACCGGTATGATCGGTGACCCGTCTATGAAG
TCTGCCGAGCGTAATTTGCTGGACGAGGCTACTCTTCGTCACAATCAGGATTGCATCAAGAAACAATTGGCTAAGTTCCT
TGATTTTGACTCCGACGCTCCTAATGCCGCTAAGTTGGTGAACAACTATGACTGGATGAAGGGTTATTCTTTCTTGAATT
TTATCCGTGATATCGGTAAGCATATTACCGTGAACTACATGATGGCTAAGGATTCAGTGAAGAAACGTTTGAGCCGCGAA
TCTAGTGTAGGTATGTCTTTCACCGAGTTTTCTTATCAATTGCTACAAGGCTACGATTATTTGTATTTGTATGAGCATGA
GGGTTGCCGTCTACAGATGGGTGGTACCGATCAATGGGGTAATATCACGACTGGTACGGAATTGATCCGTCGTACGTTGG
GAGGGGAGGCTTATGCCTTGACTTGCCCGTTGATCACGAAGGCCGACGGTGGTAAGTTCGGAAAGACGGAATCCGGTAAT
ATCTGGTTGGATCGTCGCTATACTTCTCCGTATAAATTCTATCAATTCTGGTTGAACGTAAGCGACGCTGACGCCGCCAA
GTATATCAAGATCTTCACGGACTTGAGCCAAGAAGAGATCGCCGCTTTGGAGGCGGAGCAAGAGGCCGCTCCTCATTTGC
GTCCGTTGCAGAAACGTTTGGCGAAAGAAGTGACTGTTATGGTTCACTCTTTGGAAGATTATGAGGCCGCTGTCGAGGCT
TCCAATATCTTGTTTGGTAACTCCACTCACGAGGCTTTGTTGAAATTGGATGAGGACACATTACTAGCCGTATTCGAGGG
TGTTCCTCATTTCGATATCTCCCGTGATGAATTGGCCGCAGGAATCAAGGCTGTTGATTTATGTACTGAAAAAGCCGCTA
TCTTCCCTTCAAAAGGTGAGATGCGTAAATTGGTACAAAGTGGTGGCGTAAGTTTTAACAAAGAGAAACTAGCGGAGGTT
GACACTGTAATTGATTGTTCTTCACTGCTTGACGATAAGTATTTATTGGTTCAAAGAGGTAAGAAAAATTATTATTTGTT
GATCGCAAAGTAA

Upstream 100 bases:

>100_bases
TCAATTATCAATTTTCGATTCTCAATTAAAAAGTCGTACCTTTGCCTCGTATGAGATAAAGTGACAAATATATTAATGTA
ATACGATATAAATTAATACG

Downstream 100 bases:

>100_bases
AAAAATAATCAAAAAATTTGCAAGGTAAGATTTTATTCTTACCTTTGCATCGCTTTAGTAAAGCAATCGGATTGTCTCAT
GGTGTAATGGTAGCACTACA

Product: putative tyrosyl-tRNA synthetase 1

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNFVEELRWRGMIADMMPGTEEQLQKELTSAYVGIDPTADSLHIGHLVSVMMLKHFQRAGHRPIALVGGATGMIGDPSMK
SAERNLLDEATLRHNQDCIKKQLAKFLDFDSDAPNAAKLVNNYDWMKGYSFLNFIRDIGKHITVNYMMAKDSVKKRLSRE
SSVGMSFTEFSYQLLQGYDYLYLYEHEGCRLQMGGTDQWGNITTGTELIRRTLGGEAYALTCPLITKADGGKFGKTESGN
IWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLSQEEIAALEAEQEAAPHLRPLQKRLAKEVTVMVHSLEDYEAAVEA
SNILFGNSTHEALLKLDEDTLLAVFEGVPHFDISRDELAAGIKAVDLCTEKAAIFPSKGEMRKLVQSGGVSFNKEKLAEV
DTVIDCSSLLDDKYLLVQRGKKNYYLLIAK

Sequences:

>Translated_430_residues
MNFVEELRWRGMIADMMPGTEEQLQKELTSAYVGIDPTADSLHIGHLVSVMMLKHFQRAGHRPIALVGGATGMIGDPSMK
SAERNLLDEATLRHNQDCIKKQLAKFLDFDSDAPNAAKLVNNYDWMKGYSFLNFIRDIGKHITVNYMMAKDSVKKRLSRE
SSVGMSFTEFSYQLLQGYDYLYLYEHEGCRLQMGGTDQWGNITTGTELIRRTLGGEAYALTCPLITKADGGKFGKTESGN
IWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLSQEEIAALEAEQEAAPHLRPLQKRLAKEVTVMVHSLEDYEAAVEA
SNILFGNSTHEALLKLDEDTLLAVFEGVPHFDISRDELAAGIKAVDLCTEKAAIFPSKGEMRKLVQSGGVSFNKEKLAEV
DTVIDCSSLLDDKYLLVQRGKKNYYLLIAK
>Mature_430_residues
MNFVEELRWRGMIADMMPGTEEQLQKELTSAYVGIDPTADSLHIGHLVSVMMLKHFQRAGHRPIALVGGATGMIGDPSMK
SAERNLLDEATLRHNQDCIKKQLAKFLDFDSDAPNAAKLVNNYDWMKGYSFLNFIRDIGKHITVNYMMAKDSVKKRLSRE
SSVGMSFTEFSYQLLQGYDYLYLYEHEGCRLQMGGTDQWGNITTGTELIRRTLGGEAYALTCPLITKADGGKFGKTESGN
IWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLSQEEIAALEAEQEAAPHLRPLQKRLAKEVTVMVHSLEDYEAAVEA
SNILFGNSTHEALLKLDEDTLLAVFEGVPHFDISRDELAAGIKAVDLCTEKAAIFPSKGEMRKLVQSGGVSFNKEKLAEV
DTVIDCSSLLDDKYLLVQRGKKNYYLLIAK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=435, Percent_Identity=34.9425287356322, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1787925, Length=433, Percent_Identity=46.6512702078522, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25145802, Length=416, Percent_Identity=32.9326923076923, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25145805, Length=340, Percent_Identity=30.5882352941176, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325160, Length=466, Percent_Identity=32.8326180257511, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI20130383, Length=439, Percent_Identity=36.4464692482916, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PARD8 (A6LGF1)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304387.1
- ProteinModelPortal:   A6LGF1
- SMR:   A6LGF1
- STRING:   A6LGF1
- GeneID:   5308207
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3058
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   PDIS435591:BDI_3058-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 48353; Mature: 48353

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 32-32 BINDING 172-172 BINDING 176-176 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVEELRWRGMIADMMPGTEEQLQKELTSAYVGIDPTADSLHIGHLVSVMMLKHFQRAG
CCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
HRPIALVGGATGMIGDPSMKSAERNLLDEATLRHNQDCIKKQLAKFLDFDSDAPNAAKLV
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHH
NNYDWMKGYSFLNFIRDIGKHITVNYMMAKDSVKKRLSRESSVGMSFTEFSYQLLQGYDY
CCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
LYLYEHEGCRLQMGGTDQWGNITTGTELIRRTLGGEAYALTCPLITKADGGKFGKTESGN
EEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCCCC
IWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLSQEEIAALEAEQEAAPHLRPLQKRL
EEEEECCCCCCEEEHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
AKEVTVMVHSLEDYEAAVEASNILFGNSTHEALLKLDEDTLLAVFEGVPHFDISRDELAA
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHH
GIKAVDLCTEKAAIFPSKGEMRKLVQSGGVSFNKEKLAEVDTVIDCSSLLDDKYLLVQRG
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
KKNYYLLIAK
CCCEEEEEEC
>Mature Secondary Structure
MNFVEELRWRGMIADMMPGTEEQLQKELTSAYVGIDPTADSLHIGHLVSVMMLKHFQRAG
CCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
HRPIALVGGATGMIGDPSMKSAERNLLDEATLRHNQDCIKKQLAKFLDFDSDAPNAAKLV
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHH
NNYDWMKGYSFLNFIRDIGKHITVNYMMAKDSVKKRLSRESSVGMSFTEFSYQLLQGYDY
CCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
LYLYEHEGCRLQMGGTDQWGNITTGTELIRRTLGGEAYALTCPLITKADGGKFGKTESGN
EEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCCCC
IWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLSQEEIAALEAEQEAAPHLRPLQKRL
EEEEECCCCCCEEEHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
AKEVTVMVHSLEDYEAAVEASNILFGNSTHEALLKLDEDTLLAVFEGVPHFDISRDELAA
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHH
GIKAVDLCTEKAAIFPSKGEMRKLVQSGGVSFNKEKLAEVDTVIDCSSLLDDKYLLVQRG
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
KKNYYLLIAK
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA