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The map label for this gene is nanA [H]

Identifier: 150009528

GI number: 150009528

Start: 3517147

End: 3518064

Strand: Direct

Name: nanA [H]

Synonym: BDI_2941

Alternate gene names: 150009528

Gene position: 3517147-3518064 (Clockwise)

Preceding gene: 150009527

Following gene: 150009529

Centisome position: 73.1

GC content: 45.32

Gene sequence:

>918_bases
ATGGATAAAATTAAAGGATTGATCGATGCTCCCTTTACTCCGTTCTACGAGAACGGGGAAGTTAATTACGAGCCCATTGA
AGATTATGCGAAACTTCTTGTGAAAAACGGGCTGAAAGGCGTATTCATCAATGGTTCTTCCGGAGAAGGTTATATGCTGA
CAGAGGAAGAACGCATGAAATTGGCCGAGAAATGGATGGAAGCAGCTCCGGAAGGCTTTAAGGTAATCGTGCACGTAGGT
AGCACATGCGTTAAGTCTAGCCGTAATTTAGCGGAACATGCTCAAAAGATCGGCGCTTGGGGTATTGGCGCTATGGCTAC
CCCTTTCCCTCGTATCGGTCGAATTGAGGAATTGGTAAAGTACTGCGAGGAGATCGCTTGCGGCGCTCCTGCCTTACCGT
TTTACTACTATCATATCCCTGCGTTTAACAACGCTTTCCTCCCTATGCTGGATTTCTTAAAAGCAGTAGACGGGCGTATC
CCTAATTTCGCCGGTATCAAATATACATACGAGAGCTTATACGAATACAATCAATGTCGTTTATACAAGGATGGTAAATT
CGACATGTTGCACGGACAGGATGAAACGATTCTTCCTTGCCTTGCGATGGGTGGAGCGCAAGGAGGTATCGGTGGTACCA
CAAACTATAACGGAAAAGAATTAACCGGTATCCTAGAGGCTTGGGCAGCCGGTGACTTAGAGACAGCTCGCGAGAGACAA
AACTTCTCTCAGGAGGTTATTAACGTTATCTGCAATTACCGTGGTAATATCGTCGGAGGAAAACGCATCATGAAATTGAT
CGGATTAGATCTCGGCAAGAACCGTACCCCGTTCCGGAATATGACGGACGAAGAAGAGGCCGCAATGAAAGCGGAATTAG
AGGCTATCAATTTCTTTGAAAGATGTAATAAATTCTAA

Upstream 100 bases:

>100_bases
TCATTTTCTCGATAAAACACCACTATATTATTAAAATATTTTATTTATATTTGCAGCATAATTAAAATCAGAGGTTAAAA
CTCATAAAAATAACGCAGAT

Downstream 100 bases:

>100_bases
GGTATGGCAGCGTTTGACAATTTATCTAATCCATCGGAATATCTAGCTTATTGGGGCAAGACCTATCGGAATGAGCTTCT
TTCCAATATTCTTCCTTTTT

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDKIKGLIDAPFTPFYENGEVNYEPIEDYAKLLVKNGLKGVFINGSSGEGYMLTEEERMKLAEKWMEAAPEGFKVIVHVG
STCVKSSRNLAEHAQKIGAWGIGAMATPFPRIGRIEELVKYCEEIACGAPALPFYYYHIPAFNNAFLPMLDFLKAVDGRI
PNFAGIKYTYESLYEYNQCRLYKDGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGILEAWAAGDLETARERQ
NFSQEVINVICNYRGNIVGGKRIMKLIGLDLGKNRTPFRNMTDEEEAAMKAELEAINFFERCNKF

Sequences:

>Translated_305_residues
MDKIKGLIDAPFTPFYENGEVNYEPIEDYAKLLVKNGLKGVFINGSSGEGYMLTEEERMKLAEKWMEAAPEGFKVIVHVG
STCVKSSRNLAEHAQKIGAWGIGAMATPFPRIGRIEELVKYCEEIACGAPALPFYYYHIPAFNNAFLPMLDFLKAVDGRI
PNFAGIKYTYESLYEYNQCRLYKDGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGILEAWAAGDLETARERQ
NFSQEVINVICNYRGNIVGGKRIMKLIGLDLGKNRTPFRNMTDEEEAAMKAELEAINFFERCNKF
>Mature_305_residues
MDKIKGLIDAPFTPFYENGEVNYEPIEDYAKLLVKNGLKGVFINGSSGEGYMLTEEERMKLAEKWMEAAPEGFKVIVHVG
STCVKSSRNLAEHAQKIGAWGIGAMATPFPRIGRIEELVKYCEEIACGAPALPFYYYHIPAFNNAFLPMLDFLKAVDGRI
PNFAGIKYTYESLYEYNQCRLYKDGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGILEAWAAGDLETARERQ
NFSQEVINVICNYRGNIVGGKRIMKLIGLDLGKNRTPFRNMTDEEEAAMKAELEAINFFERCNKF

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=302, Percent_Identity=36.7549668874172, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI31543060, Length=296, Percent_Identity=24.6621621621622, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789620, Length=255, Percent_Identity=29.0196078431373, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082415, Length=244, Percent_Identity=27.8688524590164, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786463, Length=235, Percent_Identity=28.0851063829787, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 34117; Mature: 34117

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIKGLIDAPFTPFYENGEVNYEPIEDYAKLLVKNGLKGVFINGSSGEGYMLTEEERMK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHH
LAEKWMEAAPEGFKVIVHVGSTCVKSSRNLAEHAQKIGAWGIGAMATPFPRIGRIEELVK
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
YCEEIACGAPALPFYYYHIPAFNNAFLPMLDFLKAVDGRIPNFAGIKYTYESLYEYNQCR
HHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
LYKDGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGILEAWAAGDLETARERQ
EEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
NFSQEVINVICNYRGNIVGGKRIMKLIGLDLGKNRTPFRNMTDEEEAAMKAELEAINFFE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RCNKF
HHCCC
>Mature Secondary Structure
MDKIKGLIDAPFTPFYENGEVNYEPIEDYAKLLVKNGLKGVFINGSSGEGYMLTEEERMK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHH
LAEKWMEAAPEGFKVIVHVGSTCVKSSRNLAEHAQKIGAWGIGAMATPFPRIGRIEELVK
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
YCEEIACGAPALPFYYYHIPAFNNAFLPMLDFLKAVDGRIPNFAGIKYTYESLYEYNQCR
HHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
LYKDGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGILEAWAAGDLETARERQ
EEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
NFSQEVINVICNYRGNIVGGKRIMKLIGLDLGKNRTPFRNMTDEEEAAMKAELEAINFFE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RCNKF
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]