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The map label for this gene is ald [H]

Identifier: 150009400

GI number: 150009400

Start: 3350639

End: 3351850

Strand: Reverse

Name: ald [H]

Synonym: BDI_2808

Alternate gene names: 150009400

Gene position: 3351850-3350639 (Counterclockwise)

Preceding gene: 150009401

Following gene: 150009399

Centisome position: 69.67

GC content: 49.17

Gene sequence:

>1212_bases
ATGGAAGCGAACGACGGCCGGCAGGCACAGGGGGCCTATATCCCGCAGGAATTATTGAAAGAGATCAGCAAGGTGAATAA
CCGTTTGCTCATCGGTATTCCTCGTGAACGATGCGAGGCGGAAAAACGTTTGCCGTTAACTCCGGAGGCGGTGGATATGC
TGACCGATTGCGGTCACCGGGTGCTGGTGGAAAGTGGCGCCGGATTAGGCATCAACTATTCCGATAATCATTTCTCGGAG
GCGGGGGCGGAGATCGTCGATACGCCCGCAGAAGTTTTCCAAGCGGATCTGATCTTGAAGATATTGCCTCCGTTACCGGT
GGAAATCGCGTTTATGAGACCTCGCACGACCGTTTTCTCATTGGTGCAGTTTAATTTATTCGCTCAAGAGGCTTTCGAGT
TGATGATGGCGAAACGGATTACCGCTATCTCTTATGAGTTGATGGCGGATGAATATAATCGTTTCCCGGTGCTGAATGTC
ACTTCCGAGATCGAGGGGGCGGCTTCCATAACGATCGCTTCCGAGCTGTTGAGCAATACCCAAGGAGGTAAAGGGATCTT
ATTAGGCGGTATCCCGGGCGTATCCCCTACGGAAGTGGTGATTATCGGGGCCGGAAACGCCGGGACCGTGGCCGCGCGTG
CCGCGTTGGCCTTGGGAGCATCCGTGAAAGTATTCGATGATGATATCAATAAATTGCGTATAATACAACAGGTCTTAGGG
CAAGGGCTTTTTACTTCCACGTTCCATCCGAATGTGCTGCATAATGCGTTTCGTAGCGCTGACGTGGTGATCGGGGCGAT
GCGTTATATTAATACGAGACATCGCTATATTATCGCTACGGACTTGGTGCGTACGATGAAAAAGGGAGCTTTGGTGATCG
ATCTGAGGGTAAGCCAAGGCGGTTGCTTTGAGACGACTTGCTGCTTGTCGAGGGAAGATCCCGCGGTGTTCGAGCAATAT
GGGGTATTACATTATTGTAAACTGAATATCAGCAACCGGGTGGCTCGTACTACCTCGATGGCTTATAGTAATATATTCGT
ACCTTTATTATTGTCGTTGGGCGACGCCGGTTCCGTGCAAGGAATGATCAAATCGGATAAGGGCTTTAGGGCGGGAGTAT
ATATGTATTATGGGAAGACAGTGAATAGTTATGTGTCTAACCATTTTAATCTATCATCTAATAATCTTGATCTATACCTC
TCTGCTTTTTGA

Upstream 100 bases:

>100_bases
GTTTTATACGAAGGCGTCCATAACGTTTGATGCCGAGAAGATGTTAACAGAATCTGATGTACATGACATATCGAACGCGT
TAATGAAAATCCTATAGCAT

Downstream 100 bases:

>100_bases
TGCGGAAAAGTTTTGAACTCGCAAAGTTTATGCTTTACTTTGCGGACGATCTAAATACCAATAAGCGATAAAGCTAATAA
AGTATATTATGGCAGAACAG

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEANDGRQAQGAYIPQELLKEISKVNNRLLIGIPRERCEAEKRLPLTPEAVDMLTDCGHRVLVESGAGLGINYSDNHFSE
AGAEIVDTPAEVFQADLILKILPPLPVEIAFMRPRTTVFSLVQFNLFAQEAFELMMAKRITAISYELMADEYNRFPVLNV
TSEIEGAASITIASELLSNTQGGKGILLGGIPGVSPTEVVIIGAGNAGTVAARAALALGASVKVFDDDINKLRIIQQVLG
QGLFTSTFHPNVLHNAFRSADVVIGAMRYINTRHRYIIATDLVRTMKKGALVIDLRVSQGGCFETTCCLSREDPAVFEQY
GVLHYCKLNISNRVARTTSMAYSNIFVPLLLSLGDAGSVQGMIKSDKGFRAGVYMYYGKTVNSYVSNHFNLSSNNLDLYL
SAF

Sequences:

>Translated_403_residues
MEANDGRQAQGAYIPQELLKEISKVNNRLLIGIPRERCEAEKRLPLTPEAVDMLTDCGHRVLVESGAGLGINYSDNHFSE
AGAEIVDTPAEVFQADLILKILPPLPVEIAFMRPRTTVFSLVQFNLFAQEAFELMMAKRITAISYELMADEYNRFPVLNV
TSEIEGAASITIASELLSNTQGGKGILLGGIPGVSPTEVVIIGAGNAGTVAARAALALGASVKVFDDDINKLRIIQQVLG
QGLFTSTFHPNVLHNAFRSADVVIGAMRYINTRHRYIIATDLVRTMKKGALVIDLRVSQGGCFETTCCLSREDPAVFEQY
GVLHYCKLNISNRVARTTSMAYSNIFVPLLLSLGDAGSVQGMIKSDKGFRAGVYMYYGKTVNSYVSNHFNLSSNNLDLYL
SAF
>Mature_403_residues
MEANDGRQAQGAYIPQELLKEISKVNNRLLIGIPRERCEAEKRLPLTPEAVDMLTDCGHRVLVESGAGLGINYSDNHFSE
AGAEIVDTPAEVFQADLILKILPPLPVEIAFMRPRTTVFSLVQFNLFAQEAFELMMAKRITAISYELMADEYNRFPVLNV
TSEIEGAASITIASELLSNTQGGKGILLGGIPGVSPTEVVIIGAGNAGTVAARAALALGASVKVFDDDINKLRIIQQVLG
QGLFTSTFHPNVLHNAFRSADVVIGAMRYINTRHRYIIATDLVRTMKKGALVIDLRVSQGGCFETTCCLSREDPAVFEQY
GVLHYCKLNISNRVARTTSMAYSNIFVPLLLSLGDAGSVQGMIKSDKGFRAGVYMYYGKTVNSYVSNHFNLSSNNLDLYL
SAF

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=358, Percent_Identity=25.6983240223464, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI122939153, Length=358, Percent_Identity=25.6983240223464, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1787887, Length=303, Percent_Identity=30.6930693069307, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17550456, Length=350, Percent_Identity=24, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 43971; Mature: 43971

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEANDGRQAQGAYIPQELLKEISKVNNRLLIGIPRERCEAEKRLPLTPEAVDMLTDCGHR
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCE
VLVESGAGLGINYSDNHFSEAGAEIVDTPAEVFQADLILKILPPLPVEIAFMRPRTTVFS
EEEECCCCCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LVQFNLFAQEAFELMMAKRITAISYELMADEYNRFPVLNVTSEIEGAASITIASELLSNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
QGGKGILLGGIPGVSPTEVVIIGAGNAGTVAARAALALGASVKVFDDDINKLRIIQQVLG
CCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHC
QGLFTSTFHPNVLHNAFRSADVVIGAMRYINTRHRYIIATDLVRTMKKGALVIDLRVSQG
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEEEEECCC
GCFETTCCLSREDPAVFEQYGVLHYCKLNISNRVARTTSMAYSNIFVPLLLSLGDAGSVQ
CCEEEEEECCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GMIKSDKGFRAGVYMYYGKTVNSYVSNHFNLSSNNLDLYLSAF
EEEECCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MEANDGRQAQGAYIPQELLKEISKVNNRLLIGIPRERCEAEKRLPLTPEAVDMLTDCGHR
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCE
VLVESGAGLGINYSDNHFSEAGAEIVDTPAEVFQADLILKILPPLPVEIAFMRPRTTVFS
EEEECCCCCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LVQFNLFAQEAFELMMAKRITAISYELMADEYNRFPVLNVTSEIEGAASITIASELLSNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
QGGKGILLGGIPGVSPTEVVIIGAGNAGTVAARAALALGASVKVFDDDINKLRIIQQVLG
CCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHC
QGLFTSTFHPNVLHNAFRSADVVIGAMRYINTRHRYIIATDLVRTMKKGALVIDLRVSQG
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEEEEECCC
GCFETTCCLSREDPAVFEQYGVLHYCKLNISNRVARTTSMAYSNIFVPLLLSLGDAGSVQ
CCEEEEEECCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GMIKSDKGFRAGVYMYYGKTVNSYVSNHFNLSSNNLDLYLSAF
EEEECCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]