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The map label for this gene is aroK

Identifier: 150009401

GI number: 150009401

Start: 3351854

End: 3352375

Strand: Reverse

Name: aroK

Synonym: BDI_2809

Alternate gene names: 150009401

Gene position: 3352375-3351854 (Counterclockwise)

Preceding gene: 150009402

Following gene: 150009400

Centisome position: 69.68

GC content: 49.04

Gene sequence:

>522_bases
ATGAAACGTGTTTTTTTGGTCGGATATATGGGAGCGGGCAAGACAACGGTGGGAAAAGAGCTTGCCAAGCTCGCCGGTCT
TTCGTTCATAGATCTTGATTACTATATCGAAGGGCGTTATCATAAGGCCGTTTCGCAGATATTCGCCGAGAGAGGGGAGG
AGGCTTTCCGAGAGATAGAACGGAATATGCTTCACGAGGTCGCTGAGTTTGAGGACGTGCTGATCTCCACGGGAGGAGGC
GCTCCTTGCTTTTTCGATAACATGGAGTTTATGAACGCTTCTGGCACGACGGTTTACTTGAAGGTGTCAGTCGAGGAGCT
GGCGAAGCGTCTGGAGTTGTGCAAACATACCCGTCCGGTGTTGAAAGGACGTTCCGGGGAGGAACTGAGGGCGTTTATCG
CCGAGAGTCTGGAGAAAAGGAATCCGTTTTATACGAAGGCGTCCATAACGTTTGATGCCGAGAAGATGTTAACAGAATCT
GATGTACATGACATATCGAACGCGTTAATGAAAATCCTATAG

Upstream 100 bases:

>100_bases
TAAAAGCCATTGGCCTATGATTTTTTTATTGATTCTTAGGAATAAAGAAGATGGAGTTCCCGGAAAAAGTTTACATTTGC
GAAAACTATTATAATAACGT

Downstream 100 bases:

>100_bases
CATATGGAAGCGAACGACGGCCGGCAGGCACAGGGGGCCTATATCCCGCAGGAATTATTGAAAGAGATCAGCAAGGTGAA
TAACCGTTTGCTCATCGGTA

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKRVFLVGYMGAGKTTVGKELAKLAGLSFIDLDYYIEGRYHKAVSQIFAERGEEAFREIERNMLHEVAEFEDVLISTGGG
APCFFDNMEFMNASGTTVYLKVSVEELAKRLELCKHTRPVLKGRSGEELRAFIAESLEKRNPFYTKASITFDAEKMLTES
DVHDISNALMKIL

Sequences:

>Translated_173_residues
MKRVFLVGYMGAGKTTVGKELAKLAGLSFIDLDYYIEGRYHKAVSQIFAERGEEAFREIERNMLHEVAEFEDVLISTGGG
APCFFDNMEFMNASGTTVYLKVSVEELAKRLELCKHTRPVLKGRSGEELRAFIAESLEKRNPFYTKASITFDAEKMLTES
DVHDISNALMKIL
>Mature_173_residues
MKRVFLVGYMGAGKTTVGKELAKLAGLSFIDLDYYIEGRYHKAVSQIFAERGEEAFREIERNMLHEVAEFEDVLISTGGG
APCFFDNMEFMNASGTTVYLKVSVEELAKRLELCKHTRPVLKGRSGEELRAFIAESLEKRNPFYTKASITFDAEKMLTES
DVHDISNALMKIL

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=151, Percent_Identity=33.7748344370861, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1786587, Length=176, Percent_Identity=34.0909090909091, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_PARD8 (A6LFQ8)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304144.1
- ProteinModelPortal:   A6LFQ8
- SMR:   A6LFQ8
- STRING:   A6LFQ8
- GeneID:   5307958
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2809
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   MIISERV
- ProtClustDB:   PRK13949
- BioCyc:   PDIS435591:BDI_2809-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19517; Mature: 19517

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 33-33 BINDING 57-57 BINDING 79-79 BINDING 118-118 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFLVGYMGAGKTTVGKELAKLAGLSFIDLDYYIEGRYHKAVSQIFAERGEEAFREIE
CCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHEECCHHHHHHHHHHHHCCHHHHHHHH
RNMLHEVAEFEDVLISTGGGAPCFFDNMEFMNASGTTVYLKVSVEELAKRLELCKHTRPV
HHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
LKGRSGEELRAFIAESLEKRNPFYTKASITFDAEKMLTESDVHDISNALMKIL
HCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKRVFLVGYMGAGKTTVGKELAKLAGLSFIDLDYYIEGRYHKAVSQIFAERGEEAFREIE
CCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHEECCHHHHHHHHHHHHCCHHHHHHHH
RNMLHEVAEFEDVLISTGGGAPCFFDNMEFMNASGTTVYLKVSVEELAKRLELCKHTRPV
HHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
LKGRSGEELRAFIAESLEKRNPFYTKASITFDAEKMLTESDVHDISNALMKIL
HCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA