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The map label for this gene is wcaE [C]

Identifier: 150009370

GI number: 150009370

Start: 3314722

End: 3315483

Strand: Reverse

Name: wcaE [C]

Synonym: BDI_2778

Alternate gene names: 150009370

Gene position: 3315483-3314722 (Counterclockwise)

Preceding gene: 150009371

Following gene: 150009369

Centisome position: 68.91

GC content: 45.14

Gene sequence:

>762_bases
ATGAATCAGCAACCTACATTTTCGATCATAACGATCACCTATAATGCGGAGCGATGGTTGGAGCGTACGATATTGAGCGT
ACTTAGCCAGTCGTATCCGAATATCGAGTATATATTGATTGACGGAGCGTCGAAGGATAAGACCGTAGAGATCATCAAGC
AATATGAGTCCGGTATCGCTTCTTGGATAAGCGAGCCTGATAAAGGCTTGTACGATGCCATGAATAAAGGTTTAAGGCGA
GCCACGGGGGACTATGTGTGGTTCTTGAATGCGGGCGATACACTTTATACCGCAGATACCGTGCAAAGAATTGTGGCGTC
TTTGAAAAAGAAAGTATCTTTGCCAGACGTGATTTACGGAGAGACCCGGATCGTTGACGCGGCGGGCAAATCGTTGGGGA
TGCGCAGGTTGAAGGCTCCGGAGAGACTCAGTTGGAAGGGTTTTCGAATGGGAATGTTGGTTTGTCACCAGTCGTTTATA
TCAAAACGGGAGATAGCTCCGTTATATAATACGGATTATCGTTTGACGGCCGATTATGATTGGTGCATCCAATGTCTGAA
GCGTGCGAAAAGAGTGCATAATACGCATCTGGTTTTGTCTGACTTCTTGGAGGAAGGATTAAGTAGCGCCAATCGGAAAG
CTTCCTTGAAAGAGCGGTATGAGATTATGTGCAAATATTATGGGAAAGTCTCGACAATCGCATTGCATGGTTGGTTCGCC
GTACGTTTCTATTTTGCCAAATGGTTTAAAGGTAGAGTATAA

Upstream 100 bases:

>100_bases
ATGAGTTTATACCTTTGCTTCTGTCAACGGGGCGTTTGACGCGCTTATATCCCAATACCCCAAATCATCCGAAACAGGCT
TATAAAACAAAGAAGGAAAA

Downstream 100 bases:

>100_bases
ATAATTAAATATAAATAAGTTTTATGCGGAGTATAATGCAAAAATGGGGGAAGCATATCGCTGCTGTAGCCGTGTTCTTG
GTGCTGACAGTGGTTTATTT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNQQPTFSIITITYNAERWLERTILSVLSQSYPNIEYILIDGASKDKTVEIIKQYESGIASWISEPDKGLYDAMNKGLRR
ATGDYVWFLNAGDTLYTADTVQRIVASLKKKVSLPDVIYGETRIVDAAGKSLGMRRLKAPERLSWKGFRMGMLVCHQSFI
SKREIAPLYNTDYRLTADYDWCIQCLKRAKRVHNTHLVLSDFLEEGLSSANRKASLKERYEIMCKYYGKVSTIALHGWFA
VRFYFAKWFKGRV

Sequences:

>Translated_253_residues
MNQQPTFSIITITYNAERWLERTILSVLSQSYPNIEYILIDGASKDKTVEIIKQYESGIASWISEPDKGLYDAMNKGLRR
ATGDYVWFLNAGDTLYTADTVQRIVASLKKKVSLPDVIYGETRIVDAAGKSLGMRRLKAPERLSWKGFRMGMLVCHQSFI
SKREIAPLYNTDYRLTADYDWCIQCLKRAKRVHNTHLVLSDFLEEGLSSANRKASLKERYEIMCKYYGKVSTIALHGWFA
VRFYFAKWFKGRV
>Mature_253_residues
MNQQPTFSIITITYNAERWLERTILSVLSQSYPNIEYILIDGASKDKTVEIIKQYESGIASWISEPDKGLYDAMNKGLRR
ATGDYVWFLNAGDTLYTADTVQRIVASLKKKVSLPDVIYGETRIVDAAGKSLGMRRLKAPERLSWKGFRMGMLVCHQSFI
SKREIAPLYNTDYRLTADYDWCIQCLKRAKRVHNTHLVLSDFLEEGLSSANRKASLKERYEIMCKYYGKVSTIALHGWFA
VRFYFAKWFKGRV

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788368, Length=217, Percent_Identity=27.1889400921659, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 29201; Mature: 29201

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQPTFSIITITYNAERWLERTILSVLSQSYPNIEYILIDGASKDKTVEIIKQYESGIA
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
SWISEPDKGLYDAMNKGLRRATGDYVWFLNAGDTLYTADTVQRIVASLKKKVSLPDVIYG
HHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCEECC
ETRIVDAAGKSLGMRRLKAPERLSWKGFRMGMLVCHQSFISKREIAPLYNTDYRLTADYD
CEEEEEHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHH
WCIQCLKRAKRVHNTHLVLSDFLEEGLSSANRKASLKERYEIMCKYYGKVSTIALHGWFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHH
VRFYFAKWFKGRV
HHHHHHHHHCCCC
>Mature Secondary Structure
MNQQPTFSIITITYNAERWLERTILSVLSQSYPNIEYILIDGASKDKTVEIIKQYESGIA
CCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
SWISEPDKGLYDAMNKGLRRATGDYVWFLNAGDTLYTADTVQRIVASLKKKVSLPDVIYG
HHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCEECC
ETRIVDAAGKSLGMRRLKAPERLSWKGFRMGMLVCHQSFISKREIAPLYNTDYRLTADYD
CEEEEEHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHH
WCIQCLKRAKRVHNTHLVLSDFLEEGLSSANRKASLKERYEIMCKYYGKVSTIALHGWFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHH
VRFYFAKWFKGRV
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]