Click here to switch to the map view.

The map label for this gene is fumB [H]

Identifier: 150009357

GI number: 150009357

Start: 3294855

End: 3296483

Strand: Reverse

Name: fumB [H]

Synonym: BDI_2765

Alternate gene names: 150009357

Gene position: 3296483-3294855 (Counterclockwise)

Preceding gene: 150009361

Following gene: 150009355

Centisome position: 68.51

GC content: 50.34

Gene sequence:

>1629_bases
ATGGCAACACCTCCGTTTCACTACGAGCCGATGTTCCAAAAGGGACCGGACACAACAGAGTATTATTTGCTTACAAAAGA
TTATGTTTCCGTTTCTGAGTTTGAAGGAAAACCGATCTTGAAAGTCGCTAAAGAAGGTTTGACCGCTATGGCTAACGCCG
CTTTCCGCGACGTATCATTCATGCTTCGCCGTTCTCACAACGAGCAGGTCGCTAAGATCCTAAGCGATCCGGAAGCTACG
GACAACGATAAGTACGTGGCCTTGACATTCCTTCGCAACGCCGAGGTAGCGGCTAAAGGCGTACTTCCTTTCTGCCAAGA
TACCGGTACGGCTATTATCCACGGCGAGAAGGGCCAGCAGGTCTGGACCGGTTACTGTGATGAAGAAGCGCTTTCCTTAG
GTGTTTACAAGACCTACACGGAAGAGAACCTTCGTTACTCCCAGAACGCTCCGCTGAATATGTACGATGAGGTAAATACG
AAATGTAACTTGCCGGCCCAGATCGATATCGAGGCTACGGAAGGCATGGAGTATGAGTTCCTTTGCGTGACAAAAGGAGG
TGGTTCCGCCAATAAGACTTATTTATACCAAGAGACAAAAGCGATCTTGAATCCGGGTACATTGGTTCCCTTCCTCGTAG
AGAAGATCAAGACATTGGGTACGGCCGCTTGTCCTCCGTATCATATAGCGATCGTGATTGGCGGTACTTCCGCTGAGAAG
AATTTATTGACGGTGAAGTTGGCATCTACCCGTTTCTATGATAGCTTGCCTACGACCGGTAGCGAGGGTGGCCGTGCTTT
CCGCGACGTGGAGTTGGAGAAAGAGGTATTGGCCGCCGCCCAGAATATTGGCTTGGGCGCTCAATTCGGTGGTAAATATC
TGGCACATGATGTTCGCATCATCCGTTTGCCTCGCCATGGCGCTTCTTGCCCGGTAGGTTTGGGTGTAAGTTGCTCTGCG
GACCGTAATATTAAATGTAAGATCAATAAGGAAGGTATCTGGATCGAGAAGTTGGATAGCCATCCCGGCGAATTGATTCC
GGAGGAACTTCGCCAAGCGGGTGAGGGCGACGCTGTCAAGATCAACTTGAACCAACCGATGGCGGATATCTTGAAGGAAT
TGGATAAATATCCGGTATCTACTCGTTTGTCATTGAACGGAACGATCATCGTGGGCCGTGATATCGCTCACGCTAAACTG
AAGGAGCGTCTGGACCGTGGCGAGGATCTCCCCCAATATATCAAGGATCATCCTATTTATTACGCGGGCCCGGCTAAGAC
TCCGGCAGGTATGGCTTGTGGCTCTATGGGCCCGACGACCGCGGGTCGTATGGACTCATACGTTGACTTGTTCCAGAGCC
ATGGCGGTAGCATGATCATGTTGGCGAAGGGTAACCGTAGCCAGCAAGTTACGGACGCTTGTAAGAATCACGGCGGTTTC
TACCTAGGATCTATCGGTGGCCCCGCGGCTATCTTGGCTCAGAACAATATCAAGAGCATCGAGTGCGTGGAATATCCGGA
ATTGGGTATGGAAGCGATCTGGAAGATCGAGGTAGAGAACTTCCCCGCGTTTATCTTGGTAGATAACAAGGGTAATGACT
TCTTCAAGCAGATCAAGCCTCGTTGCTGA

Upstream 100 bases:

>100_bases
TTACCCCATGATCTATATATCAAGTTTTTTCGCTAAGTTTGCCAATCGAAAAGTACGGACAAACTTAAATAAAACCAATA
TATAATTAAATTCTAAAGAT

Downstream 100 bases:

>100_bases
TTCCTTGATCGGTAAAGATAAATGAAAAGCGGTATCCGGCGGCAAGCTGGATACCGCTTTTTTCTAAAAAAAAAGTCGTA
CGTGTTTTTTTAATAGGTTA

Product: fumarate hydratase class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MATPPFHYEPMFQKGPDTTEYYLLTKDYVSVSEFEGKPILKVAKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEAT
DNDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNT
KCNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKIKTLGTAACPPYHIAIVIGGTSAEK
NLLTVKLASTRFYDSLPTTGSEGGRAFRDVELEKEVLAAAQNIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSA
DRNIKCKINKEGIWIEKLDSHPGELIPEELRQAGEGDAVKINLNQPMADILKELDKYPVSTRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKNHGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVENFPAFILVDNKGNDFFKQIKPRC

Sequences:

>Translated_542_residues
MATPPFHYEPMFQKGPDTTEYYLLTKDYVSVSEFEGKPILKVAKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEAT
DNDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNT
KCNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKIKTLGTAACPPYHIAIVIGGTSAEK
NLLTVKLASTRFYDSLPTTGSEGGRAFRDVELEKEVLAAAQNIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSA
DRNIKCKINKEGIWIEKLDSHPGELIPEELRQAGEGDAVKINLNQPMADILKELDKYPVSTRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKNHGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVENFPAFILVDNKGNDFFKQIKPRC
>Mature_541_residues
ATPPFHYEPMFQKGPDTTEYYLLTKDYVSVSEFEGKPILKVAKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEATD
NDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTK
CNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKIKTLGTAACPPYHIAIVIGGTSAEKN
LLTVKLASTRFYDSLPTTGSEGGRAFRDVELEKEVLAAAQNIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSAD
RNIKCKINKEGIWIEKLDSHPGELIPEELRQAGEGDAVKINLNQPMADILKELDKYPVSTRLSLNGTIIVGRDIAHAKLK
ERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKNHGGFY
LGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVENFPAFILVDNKGNDFFKQIKPRC

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=539, Percent_Identity=76.4378478664193, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=538, Percent_Identity=76.0223048327137, Blast_Score=860, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=29.6511627906977, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59416; Mature: 59285

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPPFHYEPMFQKGPDTTEYYLLTKDYVSVSEFEGKPILKVAKEGLTAMANAAFRDVSF
CCCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLRRSHNEQVAKILSDPEATDNDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCEEEECCCCCE
VWTGYCDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTKCNLPAQIDIEATEGMEYEF
EEECCCCHHHHEECEEEEECCCCCEECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCEEE
LCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKIKTLGTAACPPYHIAIVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTRFYDSLPTTGSEGGRAFRDVELEKEVLAAAQNIGLGAQFGGKYLAHDVRI
CEEEEEEECCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCEEECCEEE
IRLPRHGASCPVGLGVSCSADRNIKCKINKEGIWIEKLDSHPGELIPEELRQAGEGDAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCCEEE
INLNQPMADILKELDKYPVSTRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPAGMACGSMGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKNHGGF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVENFPAFILVDNKGNDFFKQIKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCC
RC
CC
>Mature Secondary Structure 
ATPPFHYEPMFQKGPDTTEYYLLTKDYVSVSEFEGKPILKVAKEGLTAMANAAFRDVSF
CCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLRRSHNEQVAKILSDPEATDNDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCEEEECCCCCE
VWTGYCDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTKCNLPAQIDIEATEGMEYEF
EEECCCCHHHHEECEEEEECCCCCEECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCEEE
LCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKIKTLGTAACPPYHIAIVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTRFYDSLPTTGSEGGRAFRDVELEKEVLAAAQNIGLGAQFGGKYLAHDVRI
CEEEEEEECCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCEEECCEEE
IRLPRHGASCPVGLGVSCSADRNIKCKINKEGIWIEKLDSHPGELIPEELRQAGEGDAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCCEEE
INLNQPMADILKELDKYPVSTRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPAGMACGSMGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKNHGGF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVENFPAFILVDNKGNDFFKQIKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCC
RC
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]