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The map label for this gene is apbE [H]

Identifier: 150009108

GI number: 150009108

Start: 2957029

End: 2958036

Strand: Reverse

Name: apbE [H]

Synonym: BDI_2509

Alternate gene names: 150009108

Gene position: 2958036-2957029 (Counterclockwise)

Preceding gene: 150009109

Following gene: 150009102

Centisome position: 61.48

GC content: 46.03

Gene sequence:

>1008_bases
ATGAAAAGTCAATTCTCCATTCTTTGTTTACTTGTTAATATCTTGTTGATAAGTTGTGAACAGGCTCCCCAATACTATGA
GGTATCTGGAAGGCTTCATACACCTTATCATATTAAGTTTGAGCATACTAAACCATTGGATAAGGAGATTGACGAGCAGT
TGAAATATTTCTACCACCTGTTTAATGCTTTTGATTCTACTTCGGTAATCAGTCAGGTAAACCAGAACCGTGACATACGG
GTAGATACGCTCTTCCAAAAAGTCTTTAAGAAGGCGCTTGAAGTGTCTACTGAGACAAATGGGGCCTACGACGTGACTTG
CGCCCCGTTGATCAACCTTTGGGGGTTTGGCTTCAGCCGGAAGGATAGCGTAACCCCTGCCCATATCGACAGTGTCCGTC
AGTTTATCGGTTTTCAGAAAGTGCGTTTAGAAGGGGATCGCATCGTGAAGGACGATCCTCGCTTAATGATGAACTTTTCT
TCTTTGGCGGATGGAACGGTTTGCGATATGATAGCCCAAATGCTGGAGAAGAAAGGGGTCCGTAATTATTTGGTGGAGTT
TGGTGGCGAGATGCGTGTGAAAGGCGTGAATCCCTCGGGCATGGATTGGCGCTTAGGGATCACGAAGCCTACGGATGACG
CTGCCGGCATGAATCAAGAACTCGAGCAGATCGTTAGCTTCCCGAAACCGTTAGGAATGGCTACATCAGGCAATTATCGT
AATTTTTATATAAAGGATGGACGTAAATACGCTCATACGATCGACCCCCGTGAAGGTTGCCCTGTACAGCGGGATATCCT
TAGTGCTACGATTGTCGCCCCGGATGCCATGACCGCTGATGCGTATGCTACCGCATTTATGGTCTTAGGCTCGGAGGAGG
CTAAATCCTTATGCGCTAAAGTGCCGGGTCTTGAATATTTTATTATTTGTTCCGATTCGATAGGAGACGGGTATCATCCG
GAATACTCTGAGGGCTTTCGTCAATATCTGGTGACTGCCGATAAGTAA

Upstream 100 bases:

>100_bases
GAAGGCCGTGATGTTTATTTAGCGGATTCCAGCTATGTATTGATGATGGCCCAACAGGATTTTCCGGATATTAAGTTCCA
TTTTACATCAGAGTTCTAAT

Downstream 100 bases:

>100_bases
TCGATTTATTTATAAGCTGTTAAGATGTTGTTCTTAGAATAGGCATTGTAAGATCTTTATGATGCCCATTCTAAGGAGCT
TATAATGCCCATCGTAAGCA

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKSQFSILCLLVNILLISCEQAPQYYEVSGRLHTPYHIKFEHTKPLDKEIDEQLKYFYHLFNAFDSTSVISQVNQNRDIR
VDTLFQKVFKKALEVSTETNGAYDVTCAPLINLWGFGFSRKDSVTPAHIDSVRQFIGFQKVRLEGDRIVKDDPRLMMNFS
SLADGTVCDMIAQMLEKKGVRNYLVEFGGEMRVKGVNPSGMDWRLGITKPTDDAAGMNQELEQIVSFPKPLGMATSGNYR
NFYIKDGRKYAHTIDPREGCPVQRDILSATIVAPDAMTADAYATAFMVLGSEEAKSLCAKVPGLEYFIICSDSIGDGYHP
EYSEGFRQYLVTADK

Sequences:

>Translated_335_residues
MKSQFSILCLLVNILLISCEQAPQYYEVSGRLHTPYHIKFEHTKPLDKEIDEQLKYFYHLFNAFDSTSVISQVNQNRDIR
VDTLFQKVFKKALEVSTETNGAYDVTCAPLINLWGFGFSRKDSVTPAHIDSVRQFIGFQKVRLEGDRIVKDDPRLMMNFS
SLADGTVCDMIAQMLEKKGVRNYLVEFGGEMRVKGVNPSGMDWRLGITKPTDDAAGMNQELEQIVSFPKPLGMATSGNYR
NFYIKDGRKYAHTIDPREGCPVQRDILSATIVAPDAMTADAYATAFMVLGSEEAKSLCAKVPGLEYFIICSDSIGDGYHP
EYSEGFRQYLVTADK
>Mature_335_residues
MKSQFSILCLLVNILLISCEQAPQYYEVSGRLHTPYHIKFEHTKPLDKEIDEQLKYFYHLFNAFDSTSVISQVNQNRDIR
VDTLFQKVFKKALEVSTETNGAYDVTCAPLINLWGFGFSRKDSVTPAHIDSVRQFIGFQKVRLEGDRIVKDDPRLMMNFS
SLADGTVCDMIAQMLEKKGVRNYLVEFGGEMRVKGVNPSGMDWRLGITKPTDDAAGMNQELEQIVSFPKPLGMATSGNYR
NFYIKDGRKYAHTIDPREGCPVQRDILSATIVAPDAMTADAYATAFMVLGSEEAKSLCAKVPGLEYFIICSDSIGDGYHP
EYSEGFRQYLVTADK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=357, Percent_Identity=29.9719887955182, Blast_Score=180, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37748; Mature: 37748

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQFSILCLLVNILLISCEQAPQYYEVSGRLHTPYHIKFEHTKPLDKEIDEQLKYFYHL
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCEEEEECCCCCCHHHHHHHHHHHHHH
FNAFDSTSVISQVNQNRDIRVDTLFQKVFKKALEVSTETNGAYDVTCAPLINLWGFGFSR
HHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHCCCCCC
KDSVTPAHIDSVRQFIGFQKVRLEGDRIVKDDPRLMMNFSSLADGTVCDMIAQMLEKKGV
CCCCCHHHHHHHHHHHCCEEEEECCCEEECCCCHHEEEHHHHCCCHHHHHHHHHHHHCCH
RNYLVEFGGEMRVKGVNPSGMDWRLGITKPTDDAAGMNQELEQIVSFPKPLGMATSGNYR
HHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
NFYIKDGRKYAHTIDPREGCPVQRDILSATIVAPDAMTADAYATAFMVLGSEEAKSLCAK
EEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHEECCHHHHHHHHH
VPGLEYFIICSDSIGDGYHPEYSEGFRQYLVTADK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSQFSILCLLVNILLISCEQAPQYYEVSGRLHTPYHIKFEHTKPLDKEIDEQLKYFYHL
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCEEEEECCCCCCHHHHHHHHHHHHHH
FNAFDSTSVISQVNQNRDIRVDTLFQKVFKKALEVSTETNGAYDVTCAPLINLWGFGFSR
HHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHCCCCCC
KDSVTPAHIDSVRQFIGFQKVRLEGDRIVKDDPRLMMNFSSLADGTVCDMIAQMLEKKGV
CCCCCHHHHHHHHHHHCCEEEEECCCEEECCCCHHEEEHHHHCCCHHHHHHHHHHHHCCH
RNYLVEFGGEMRVKGVNPSGMDWRLGITKPTDDAAGMNQELEQIVSFPKPLGMATSGNYR
HHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
NFYIKDGRKYAHTIDPREGCPVQRDILSATIVAPDAMTADAYATAFMVLGSEEAKSLCAK
EEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHEECCHHHHHHHHH
VPGLEYFIICSDSIGDGYHPEYSEGFRQYLVTADK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9473043; 11677609; 1904855 [H]