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The map label for this gene is prfC [H]

Identifier: 150009109

GI number: 150009109

Start: 2958038

End: 2959618

Strand: Reverse

Name: prfC [H]

Synonym: BDI_2510

Alternate gene names: 150009109

Gene position: 2959618-2958038 (Counterclockwise)

Preceding gene: 150009110

Following gene: 150009108

Centisome position: 61.51

GC content: 45.86

Gene sequence:

>1581_bases
ATGAGCCAGTATTCAGAAGAGATTGAAAGAAGAAGAACGTTTGCCATAATCAGCCACCCGGATGCCGGTAAGACTACGCT
TACCGAGAAATTATTGTTATTTGGTGGCGCTATCCATGTAGCGGGAGCGGTGAAGTCTAATAAAATCAAGAAGACTGCCA
CGTCCGACTGGATGGAGATTGAGAAGCAACGCGGTATCTCCGTAGCTACCTCCGTAATGGCTTTTGATTATTCCGATCAT
AAGATTAATATCTTGGATACACCGGGCCACCAAGATTTCGCCGAGGATACGTTCCGTACCTTGACGGCGGTGGATAGCGT
TATTATCGTGATCGATATCGCTAAGGGAGTAGAGGCGCAGACTCGTAAGTTGATGGAGGTTTGCCGTATGCGTAAGACTC
CGGTGATCGTTTTTGTCAACAAGATGGACCGTGATGGTAAAGATCCTTTTGATTTGCTGGATGAGATAGAAGAAGAATTA
CAGATAAAGGTACGCCCATTGAGTTGGCCGATCGATATGGGACAACGTTTCCGGGGCGTATACAATATTTTCGAGCAGAA
ACTGAACCTATATACCCCGAGTAAGCAATATGTGACGGAGAATGTGGAATTCAAGGATATCACGAGTCCGGATCTCGAGA
CTTATATTGATCCTACGCAAGCTGCGAAGCTTCGTGAGGATATAGAGTTGATAGAGGGGGTTTATCCCGAATTTGATGTG
AATACGTATTTGGATGGGGATATCGCTCCAGTATTCTTTGGCTCCGCTCTGAATAACTTCGGTGTGAAAGAGCTATTGGA
TTGCTTTATCCGTATCGCTCCCTCTCCACGCCCGATCCAAGCGGTAGAGCGTGTGGTAGACCCGAATGAGGATAATTTCA
CCGGCTTCATTTTCAAGATCCATGCGAATATGGACCCGAACCATCGCAGTTGTATCGCTTTTGTGAAGATTTGTTCCGGT
CGTTTTGAGCGTAACGCGAACTATAAGCATGTACGCTTCGGTAAGATGATGCGTTTCAGTAGCCCAACGGCTTTCATGGC
CCAGAAGAAAGAAGTGGTGGATGAGGCGTTTGCGGGTGATATTATCGGTTTGCCGGATACCGGGAACTTTAAGATAGGGG
ATACGTTGACTTCCGGAGAGGAATTGCATTTCAAGGGATTGCCGAGCTTCTCGCCCGAGATGTTCAAATATATAGAGAAC
GCCGATCCGATGAAGGCAAAGCAATTGAACAAGGGTATTGAGCAATTGATGGACGAGGGTGTCGCCCAGTTGTTTACCAA
TCAATTCAATGGCCGGAAGATTATCGGTACGGTGGGACAATTGCAGTTTGAGGTTATTCAATACCGTCTGTTGCATGAGT
ATGCCGCTCAATGTAAATGGGAGCCGATCAGCCTATACAAGGCTTGCTGGATCGAGAGTGATAACAAACAGGCATTGGAA
AATTTCAAGAAACGTAAGGCGCAATATATGGCGTTGGATAAGGAAGGCCGTGATGTTTATTTAGCGGATTCCAGCTATGT
ATTGATGATGGCCCAACAGGATTTTCCGGATATTAAGTTCCATTTTACATCAGAGTTCTAA

Upstream 100 bases:

>100_bases
AAAGCACGTACGGACTGACGATTCCCCATTGGGAGACTAGCTTGGCCTACTGCATGAATAACAAAGTGTAACTGATAACA
ATATATACATATAAAATAGA

Downstream 100 bases:

>100_bases
TATGAAAAGTCAATTCTCCATTCTTTGTTTACTTGTTAATATCTTGTTGATAAGTTGTGAACAGGCTCCCCAATACTATG
AGGTATCTGGAAGGCTTCAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSQYSEEIERRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVKSNKIKKTATSDWMEIEKQRGISVATSVMAFDYSDH
KINILDTPGHQDFAEDTFRTLTAVDSVIIVIDIAKGVEAQTRKLMEVCRMRKTPVIVFVNKMDRDGKDPFDLLDEIEEEL
QIKVRPLSWPIDMGQRFRGVYNIFEQKLNLYTPSKQYVTENVEFKDITSPDLETYIDPTQAAKLREDIELIEGVYPEFDV
NTYLDGDIAPVFFGSALNNFGVKELLDCFIRIAPSPRPIQAVERVVDPNEDNFTGFIFKIHANMDPNHRSCIAFVKICSG
RFERNANYKHVRFGKMMRFSSPTAFMAQKKEVVDEAFAGDIIGLPDTGNFKIGDTLTSGEELHFKGLPSFSPEMFKYIEN
ADPMKAKQLNKGIEQLMDEGVAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLHEYAAQCKWEPISLYKACWIESDNKQALE
NFKKRKAQYMALDKEGRDVYLADSSYVLMMAQQDFPDIKFHFTSEF

Sequences:

>Translated_526_residues
MSQYSEEIERRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVKSNKIKKTATSDWMEIEKQRGISVATSVMAFDYSDH
KINILDTPGHQDFAEDTFRTLTAVDSVIIVIDIAKGVEAQTRKLMEVCRMRKTPVIVFVNKMDRDGKDPFDLLDEIEEEL
QIKVRPLSWPIDMGQRFRGVYNIFEQKLNLYTPSKQYVTENVEFKDITSPDLETYIDPTQAAKLREDIELIEGVYPEFDV
NTYLDGDIAPVFFGSALNNFGVKELLDCFIRIAPSPRPIQAVERVVDPNEDNFTGFIFKIHANMDPNHRSCIAFVKICSG
RFERNANYKHVRFGKMMRFSSPTAFMAQKKEVVDEAFAGDIIGLPDTGNFKIGDTLTSGEELHFKGLPSFSPEMFKYIEN
ADPMKAKQLNKGIEQLMDEGVAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLHEYAAQCKWEPISLYKACWIESDNKQALE
NFKKRKAQYMALDKEGRDVYLADSSYVLMMAQQDFPDIKFHFTSEF
>Mature_525_residues
SQYSEEIERRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVKSNKIKKTATSDWMEIEKQRGISVATSVMAFDYSDHK
INILDTPGHQDFAEDTFRTLTAVDSVIIVIDIAKGVEAQTRKLMEVCRMRKTPVIVFVNKMDRDGKDPFDLLDEIEEELQ
IKVRPLSWPIDMGQRFRGVYNIFEQKLNLYTPSKQYVTENVEFKDITSPDLETYIDPTQAAKLREDIELIEGVYPEFDVN
TYLDGDIAPVFFGSALNNFGVKELLDCFIRIAPSPRPIQAVERVVDPNEDNFTGFIFKIHANMDPNHRSCIAFVKICSGR
FERNANYKHVRFGKMMRFSSPTAFMAQKKEVVDEAFAGDIIGLPDTGNFKIGDTLTSGEELHFKGLPSFSPEMFKYIENA
DPMKAKQLNKGIEQLMDEGVAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLHEYAAQCKWEPISLYKACWIESDNKQALEN
FKKRKAQYMALDKEGRDVYLADSSYVLMMAQQDFPDIKFHFTSEF

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=484, Percent_Identity=26.2396694214876, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI19923640, Length=522, Percent_Identity=25.2873563218391, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI25306283, Length=404, Percent_Identity=27.4752475247525, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI25306287, Length=491, Percent_Identity=24.6435845213849, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=32.7044025157233, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=26.8456375838926, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310132016, Length=129, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310110807, Length=129, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310123363, Length=129, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790835, Length=526, Percent_Identity=48.6692015209125, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=29.7570850202429, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI48994988, Length=211, Percent_Identity=33.6492890995261, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=505, Percent_Identity=26.1386138613861, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=37.0786516853933, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=30.6569343065693, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=25.4658385093168, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=24.6346555323591, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322359, Length=526, Percent_Identity=24.9049429657795, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=25.625, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=25.625, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582462, Length=495, Percent_Identity=26.2626262626263, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI221458488, Length=520, Percent_Identity=25.7692307692308, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=33.125, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60133; Mature: 60002

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYSEEIERRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVKSNKIKKTATSDWMEI
CCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCHHHEEECHHCCCCCHHHHHHHHHH
EKQRGISVATSVMAFDYSDHKINILDTPGHQDFAEDTFRTLTAVDSVIIVIDIAKGVEAQ
HHHCCCHHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHH
TRKLMEVCRMRKTPVIVFVNKMDRDGKDPFDLLDEIEEELQIKVRPLSWPIDMGQRFRGV
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
YNIFEQKLNLYTPSKQYVTENVEFKDITSPDLETYIDPTQAAKLREDIELIEGVYPEFDV
HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
NTYLDGDIAPVFFGSALNNFGVKELLDCFIRIAPSPRPIQAVERVVDPNEDNFTGFIFKI
CEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEE
HANMDPNHRSCIAFVKICSGRFERNANYKHVRFGKMMRFSSPTAFMAQKKEVVDEAFAGD
ECCCCCCHHHHHHHHHHHHCHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHCCC
IIGLPDTGNFKIGDTLTSGEELHFKGLPSFSPEMFKYIENADPMKAKQLNKGIEQLMDEG
EEECCCCCCEEECCCCCCCCCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLHEYAAQCKWEPISLYKACWIESDNKQALE
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHH
NFKKRKAQYMALDKEGRDVYLADSSYVLMMAQQDFPDIKFHFTSEF
HHHHHHHHEEEECCCCCEEEEECCCEEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure 
SQYSEEIERRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVKSNKIKKTATSDWMEI
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCHHHEEECHHCCCCCHHHHHHHHHH
EKQRGISVATSVMAFDYSDHKINILDTPGHQDFAEDTFRTLTAVDSVIIVIDIAKGVEAQ
HHHCCCHHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHH
TRKLMEVCRMRKTPVIVFVNKMDRDGKDPFDLLDEIEEELQIKVRPLSWPIDMGQRFRGV
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
YNIFEQKLNLYTPSKQYVTENVEFKDITSPDLETYIDPTQAAKLREDIELIEGVYPEFDV
HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
NTYLDGDIAPVFFGSALNNFGVKELLDCFIRIAPSPRPIQAVERVVDPNEDNFTGFIFKI
CEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEE
HANMDPNHRSCIAFVKICSGRFERNANYKHVRFGKMMRFSSPTAFMAQKKEVVDEAFAGD
ECCCCCCHHHHHHHHHHHHCHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHCCC
IIGLPDTGNFKIGDTLTSGEELHFKGLPSFSPEMFKYIENADPMKAKQLNKGIEQLMDEG
EEECCCCCCEEECCCCCCCCCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLHEYAAQCKWEPISLYKACWIESDNKQALE
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHH
NFKKRKAQYMALDKEGRDVYLADSSYVLMMAQQDFPDIKFHFTSEF
HHHHHHHHEEEECCCCCEEEEECCCEEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA