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The map label for this gene is truB

Identifier: 150009010

GI number: 150009010

Start: 2819048

End: 2819755

Strand: Reverse

Name: truB

Synonym: BDI_2406

Alternate gene names: 150009010

Gene position: 2819755-2819048 (Counterclockwise)

Preceding gene: 150009011

Following gene: 150009009

Centisome position: 58.61

GC content: 45.2

Gene sequence:

>708_bases
ATGGATTTTATAGCTGGAGAGATTTTATATTTTAATAAACCGCTAACGTGGACTTCTTTTGACCTAGTTAACAAATTTCG
CTATAAGCTAAGTCGGAAACTGAAGGTAAAGAAGATTAAAGTTGGACATGCGGGGACACTGGATCCCTTGGCTACAGGTG
TGATGATCGTTTGTACCGGAAGGGCTACGAAGCGAATCGATGAGTTTCAATACCAGACGAAGGAGTATATCGCTACGTTG
AAGTTGGGAGAGACTACCCCGTCTTTCGATTTGGAGAAAGAGATAGACGCCGTGTATCCCACGGAACATATAACCCGTGA
GCTCGTGGAGGAGGTTTTAAAGACATTCGTGGGTACGATCCAACAGATTCCCCCGGTGTTCTCCGCTTGCAAGGTGGAAG
GTAAGCGTGCTTATGAGCTAGCTCGGAAAGGAGAGGAGGTTGAACTAAAATCCAAGACATTGGTTATAGACGAACTGGAG
TTGCTGGAGTGTGATTTGCCTGTTATAAAGATACGGGTGGTATGTAGCAAAGGTACCTATATTCGTGCGTTGGCTCGTGA
TATCGGGAAGGCCTTGGGTTCCGGCGCTCATTTGATTGGCTTGGAACGGACTCGCATCGGAGAGGTAACCTTGGATATGT
GTATGTCTCCGGAGGAGATCGATGATTTCTTAGAGCAGAATGTGGTAAAAATAGAAGAAGAAAAATAA

Upstream 100 bases:

>100_bases
GATGATCAACATCGTGAAAAAGGGGAAACTGATTTATTTCGCTATCTATTGTGCGATTGCGGGATTGGTGACAATTGCTT
GTACATTACTTAAATAAAGA

Downstream 100 bases:

>100_bases
ATAGAGTGCATTATGAAGCTTTCGAAATTCAAATTTAACTTACCGTCGGAATTAATCGCTTTACATCCGGCGAAGAACAG
AGATGAGTCGAGGTTGATGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MDFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTGRATKRIDEFQYQTKEYIATL
KLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTIQQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELE
LLECDLPVIKIRVVCSKGTYIRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEEIDDFLEQNVVKIEEEK

Sequences:

>Translated_235_residues
MDFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTGRATKRIDEFQYQTKEYIATL
KLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTIQQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELE
LLECDLPVIKIRVVCSKGTYIRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEEIDDFLEQNVVKIEEEK
>Mature_235_residues
MDFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTGRATKRIDEFQYQTKEYIATL
KLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTIQQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELE
LLECDLPVIKIRVVCSKGTYIRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEEIDDFLEQNVVKIEEEK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=233, Percent_Identity=34.7639484978541, Blast_Score=131, Evalue=5e-31,
Organism=Escherichia coli, GI2367200, Length=221, Percent_Identity=33.9366515837104, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17553978, Length=209, Percent_Identity=28.7081339712919, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324037, Length=116, Percent_Identity=40.5172413793103, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364189, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364187, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364185, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364183, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62471759, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17975520, Length=208, Percent_Identity=28.8461538461538, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PARD8 (A6LEL7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303753.1
- ProteinModelPortal:   A6LEL7
- SMR:   A6LEL7
- STRING:   A6LEL7
- GeneID:   5307555
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2406
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   EYLGTIR
- ProtClustDB:   CLSK2516788
- BioCyc:   PDIS435591:BDI_2406-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26602; Mature: 26602

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTG
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHCCEEEEECC
RATKRIDEFQYQTKEYIATLKLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTI
CHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELELLECDLPVIKIRVVCSKGTY
HHCCHHHHHHHCCCHHHHHHHHCCCCEEECCCEEEEECHHHHHCCCCEEEEEEEECCCHH
IRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEEIDDFLEQNVVKIEEEK
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure
MDFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTG
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHCCEEEEECC
RATKRIDEFQYQTKEYIATLKLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTI
CHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELELLECDLPVIKIRVVCSKGTY
HHCCHHHHHHHCCCHHHHHHHHCCCCEEECCCEEEEECHHHHHCCCCEEEEEEEECCCHH
IRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEEIDDFLEQNVVKIEEEK
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA