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The map label for this gene is queA

Identifier: 150009009

GI number: 150009009

Start: 2817986

End: 2819035

Strand: Reverse

Name: queA

Synonym: BDI_2405

Alternate gene names: 150009009

Gene position: 2819035-2817986 (Counterclockwise)

Preceding gene: 150009010

Following gene: 150009008

Centisome position: 58.59

GC content: 46.86

Gene sequence:

>1050_bases
ATGAAGCTTTCGAAATTCAAATTTAACTTACCGTCGGAATTAATCGCTTTACATCCGGCGAAGAACAGAGATGAGTCGAG
GTTGATGGTCGTTCATCGTGATACGGGTGAGATCGAGCACCGCGAATTCAAGGATATTCTAGACTATTATGGAAAAGGTG
ACGTGTTTATCTTTAATAACACGAAAGTTTTCCCCGCTCGCTTGTACGGTAACAAGGAGAAAACAGGGGCGCGTATCGAG
GTGTTCTTGTTGCGCGAGCTGAACGAGGACTTACGTTTGTGGGACGTATTGGTGGATCCGGCTCGTAAGATCCGTATCGG
TAATAAGTTATACTTTGGCGAGGATGATTCCATGGTCGCGGAGGTAATTGATAATACGACTTCGCGTGGTCGTACGCTCC
GTTTCTTATATGACGGGAACCACGACGAGTTTAAGAAGGCTTTGTATGCCTTGGGCGAGACTCCGCTGCCTAAGTATATC
GAACGTGAGGTGGAACCGGAAGACGAGGATCGTTATCAGAATATTTTCGCTGCGGAAGAAGGTGCGGTTGTGGCTCCTGC
TGCGGGATTGCATTTTAGCCGTGAGTTGATGAAACGTTTGGAGATCAAGGATTGCCAGTTCGCTTTCTTGACATTGCATT
CGGGCTTGGGTAATTTCCGTGAGATCGACGTGGAAGATTTGACAAAGCATAAAATGGATTCTGAGCAAATGGTCGTAAAT
GCCGATGTAGTGGATATCGTGAACAAGGGAAAGGATGAGGGGCACAAGGTTTGTGCGGTCGGAACTTCCGTGATGCGTGC
GATCGAGACGGCCGTAAGTACGGATGGTCATCTGAAAGAGTTCGAGGGCTGGACGAATAAGTTTATCTTCCCTCCGTATG
ATTTCAGCGTGGCTACCAGCATGGTGACAAATTTCCACATGCCGTTGTCTACATTATTGATGATGACAGCTTCTTTCGGT
GGTTATGAGTTGATCATGGACGCTTACGATATCGCTTTGAAAGAGAAATATCGTTTCGGTGCTTACGGCGACGCTATGCT
AATCCTTTAA

Upstream 100 bases:

>100_bases
AGAGGTAACCTTGGATATGTGTATGTCTCCGGAGGAGATCGATGATTTCTTAGAGCAGAATGTGGTAAAAATAGAAGAAG
AAAAATAAATAGAGTGCATT

Downstream 100 bases:

>100_bases
TCCGGTTTCTATGGCGACAGCTTACCTTGGATTAGGTACGAACGTAGGTAATAAACGAGGAAATTTGGTGACAGCGGCGG
CGCTGCTGGCTGAAAGGGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLSKFKFNLPSELIALHPAKNRDESRLMVVHRDTGEIEHREFKDILDYYGKGDVFIFNNTKVFPARLYGNKEKTGARIE
VFLLRELNEDLRLWDVLVDPARKIRIGNKLYFGEDDSMVAEVIDNTTSRGRTLRFLYDGNHDEFKKALYALGETPLPKYI
EREVEPEDEDRYQNIFAAEEGAVVAPAAGLHFSRELMKRLEIKDCQFAFLTLHSGLGNFREIDVEDLTKHKMDSEQMVVN
ADVVDIVNKGKDEGHKVCAVGTSVMRAIETAVSTDGHLKEFEGWTNKFIFPPYDFSVATSMVTNFHMPLSTLLMMTASFG
GYELIMDAYDIALKEKYRFGAYGDAMLIL

Sequences:

>Translated_349_residues
MKLSKFKFNLPSELIALHPAKNRDESRLMVVHRDTGEIEHREFKDILDYYGKGDVFIFNNTKVFPARLYGNKEKTGARIE
VFLLRELNEDLRLWDVLVDPARKIRIGNKLYFGEDDSMVAEVIDNTTSRGRTLRFLYDGNHDEFKKALYALGETPLPKYI
EREVEPEDEDRYQNIFAAEEGAVVAPAAGLHFSRELMKRLEIKDCQFAFLTLHSGLGNFREIDVEDLTKHKMDSEQMVVN
ADVVDIVNKGKDEGHKVCAVGTSVMRAIETAVSTDGHLKEFEGWTNKFIFPPYDFSVATSMVTNFHMPLSTLLMMTASFG
GYELIMDAYDIALKEKYRFGAYGDAMLIL
>Mature_349_residues
MKLSKFKFNLPSELIALHPAKNRDESRLMVVHRDTGEIEHREFKDILDYYGKGDVFIFNNTKVFPARLYGNKEKTGARIE
VFLLRELNEDLRLWDVLVDPARKIRIGNKLYFGEDDSMVAEVIDNTTSRGRTLRFLYDGNHDEFKKALYALGETPLPKYI
EREVEPEDEDRYQNIFAAEEGAVVAPAAGLHFSRELMKRLEIKDCQFAFLTLHSGLGNFREIDVEDLTKHKMDSEQMVVN
ADVVDIVNKGKDEGHKVCAVGTSVMRAIETAVSTDGHLKEFEGWTNKFIFPPYDFSVATSMVTNFHMPLSTLLMMTASFG
GYELIMDAYDIALKEKYRFGAYGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=39.4285714285714, Blast_Score=251, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PARD8 (A6LEL6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303752.1
- ProteinModelPortal:   A6LEL6
- SMR:   A6LEL6
- STRING:   A6LEL6
- GeneID:   5307554
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2405
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   RKVRVGN
- ProtClustDB:   PRK00147
- BioCyc:   PDIS435591:BDI_2405-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39926; Mature: 39926

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKFKFNLPSELIALHPAKNRDESRLMVVHRDTGEIEHREFKDILDYYGKGDVFIFNN
CCCCCEECCCCHHHEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEC
TKVFPARLYGNKEKTGARIEVFLLRELNEDLRLWDVLVDPARKIRIGNKLYFGEDDSMVA
CEEEEEEECCCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCHHHEEECCEEEECCCCHHHH
EVIDNTTSRGRTLRFLYDGNHDEFKKALYALGETPLPKYIEREVEPEDEDRYQNIFAAEE
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHCCCC
GAVVAPAAGLHFSRELMKRLEIKDCQFAFLTLHSGLGNFREIDVEDLTKHKMDSEQMVVN
CCEEECCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCEECCHHHHHHHCCCCCCEEEE
ADVVDIVNKGKDEGHKVCAVGTSVMRAIETAVSTDGHLKEFEGWTNKFIFPPYDFSVATS
CHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCCCCHHHHHH
MVTNFHMPLSTLLMMTASFGGYELIMDAYDIALKEKYRFGAYGDAMLIL
HHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEC
>Mature Secondary Structure
MKLSKFKFNLPSELIALHPAKNRDESRLMVVHRDTGEIEHREFKDILDYYGKGDVFIFNN
CCCCCEECCCCHHHEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEC
TKVFPARLYGNKEKTGARIEVFLLRELNEDLRLWDVLVDPARKIRIGNKLYFGEDDSMVA
CEEEEEEECCCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCHHHEEECCEEEECCCCHHHH
EVIDNTTSRGRTLRFLYDGNHDEFKKALYALGETPLPKYIEREVEPEDEDRYQNIFAAEE
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHCCCC
GAVVAPAAGLHFSRELMKRLEIKDCQFAFLTLHSGLGNFREIDVEDLTKHKMDSEQMVVN
CCEEECCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCEECCHHHHHHHCCCCCCEEEE
ADVVDIVNKGKDEGHKVCAVGTSVMRAIETAVSTDGHLKEFEGWTNKFIFPPYDFSVATS
CHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECCCCCHHHHHH
MVTNFHMPLSTLLMMTASFGGYELIMDAYDIALKEKYRFGAYGDAMLIL
HHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA