Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 150008894

GI number: 150008894

Start: 2679783

End: 2681300

Strand: Reverse

Name: gltX

Synonym: BDI_2289

Alternate gene names: 150008894

Gene position: 2681300-2679783 (Counterclockwise)

Preceding gene: 150008900

Following gene: 150008893

Centisome position: 55.73

GC content: 50.99

Gene sequence:

>1518_bases
ATGACTCAAAGAAAAGTAAGAGTACGCTTCGCACCTAGTCCTACGGGGGCGCTGCATATCGGAGGTGTCCGCACGGCTTT
ATACAACTATTTATTCGCTAAACAGAATGGCGGTGATATGATTCTCCGTATCGAGGATACGGATTCCCAACGCTTCGTTC
CCGGGGCGGAAGACTATATCATCGAGGCCTTGACTTGGCTGGGTATCAAGTTCGACGAGGGCGTGAGCTTCGGCGGCAAC
TACGGTCCGTATCGCCAGAGCGAGCGTCGTGAGATCTACAAGAAGTATGTCGATCAATTGCTGAACGATGGCCATGCTTA
TATAGCGTTCGATACGCCTGCCGAGTTGGAGGAGAAACGGAAGGAGATCGCCAATTTCCAATACGACGCCTCTACCCGTT
CGCAAATGCGCAACTCGTTGACGCTTTCTCAGGAAGAGGTTCAGAGCCTGATCGAGTCAGGTCATCAGTACGTAGTTCGT
GCCAAGATCGAGCCGAATGAGGATATCCACGTGAACGACTTGATCCGTGGCGAGGTCGTTATCAACTCCTCTATCTTGGA
TGATAAGGTATTATATAAATCGGCCGACCAATTGCCTACTTACCACTTGGCGAATATCGTTGACGACCATTTGATGGAGG
TTACCCATGTGATCCGTGGTGAGGAGTGGTTGCCGAGCGCGCCGCTTCACGTATTATTATATCGTTTCTTCGGTTGGGAA
GATACGATGCCTAGTTTCGCCCACCTGTCGTTGCTGTTGAAACCGGAAGGTAATGGTAAGTTGAGCAAGCGTGACGGCGA
TCGTCTGGGCTTCCCTGTATTCCCCTTGGAATGGCACGATCCGAAGACGGGTGATGTCTCATCGGGCTATCGTGAGAGTG
GCTACTTCCCCGAGGCTGTCGTAAACTTCCTCGCTTTGTTGGGTTGGAACCCGGGTAACGATCAGGAGGTGATGAGCATG
GATGATTTGATCCGTTTGTTCGACCTGTCTCGTTGCAGCAAGAGCGGCGCTAAGTTTGATTATGAGAAAGGCAGATGGTT
TAACCACCACTATTTACTGGAGAAGAGTAACGCGGAGATCGCCGACCTGTTCTTGCCGATCGTGGAGAGCCACGGTATCC
AGACTACCCATGCTTATGTAGAGAAAGTAGTCGGCATGATGAAAGGCCGTGTCAATTTCGTGAGTGAGTTATGGGATCTT
TGCTCTTTCTTCTTTATCGCCCCGACGGAGTACGATGAGAAGACTCGCAAGAAACGCTGGAAGGAAGATTCGGCTGTTCA
ATTGGGTGAGTTTATCGAGCAACTGCGTGCCCGTGAGCCGTTCGACGTAGAAGGTACCGAGAACGAGTGCAAGGCTTGGA
TCGAGAGCAAGGGATATCATTTGGGGAATATCATGAACGCCGCCCGTCTGGCCTTGGTTGGCGAGGGCAAGGGCCCCGGC
ATCTTTGATATTACCGAGGCTTTAGGTAAAGAGGAGTCTATCCGCCGTATCCAACGGGCTATCGAGATATTAAAATAA

Upstream 100 bases:

>100_bases
TTCATATAGATTCCTTTTTGCGTCCGAAATATACGTAAAAAACTTCACTAAGCGGAAAAAAAGGCGTACTTTTGCCGCAT
TATTATAGAATGTAAGTATT

Downstream 100 bases:

>100_bases
TCATGTACAGTTTAGCAATACATCTCTACGCTTTTGTAGTCGCTTTGATCTCGCCTTTCCATAAAAAGGCGAGACTGATG
CGATTCGGGCAATGGAAAAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTQRKVRVRFAPSPTGALHIGGVRTALYNYLFAKQNGGDMILRIEDTDSQRFVPGAEDYIIEALTWLGIKFDEGVSFGGN
YGPYRQSERREIYKKYVDQLLNDGHAYIAFDTPAELEEKRKEIANFQYDASTRSQMRNSLTLSQEEVQSLIESGHQYVVR
AKIEPNEDIHVNDLIRGEVVINSSILDDKVLYKSADQLPTYHLANIVDDHLMEVTHVIRGEEWLPSAPLHVLLYRFFGWE
DTMPSFAHLSLLLKPEGNGKLSKRDGDRLGFPVFPLEWHDPKTGDVSSGYRESGYFPEAVVNFLALLGWNPGNDQEVMSM
DDLIRLFDLSRCSKSGAKFDYEKGRWFNHHYLLEKSNAEIADLFLPIVESHGIQTTHAYVEKVVGMMKGRVNFVSELWDL
CSFFFIAPTEYDEKTRKKRWKEDSAVQLGEFIEQLRAREPFDVEGTENECKAWIESKGYHLGNIMNAARLALVGEGKGPG
IFDITEALGKEESIRRIQRAIEILK

Sequences:

>Translated_505_residues
MTQRKVRVRFAPSPTGALHIGGVRTALYNYLFAKQNGGDMILRIEDTDSQRFVPGAEDYIIEALTWLGIKFDEGVSFGGN
YGPYRQSERREIYKKYVDQLLNDGHAYIAFDTPAELEEKRKEIANFQYDASTRSQMRNSLTLSQEEVQSLIESGHQYVVR
AKIEPNEDIHVNDLIRGEVVINSSILDDKVLYKSADQLPTYHLANIVDDHLMEVTHVIRGEEWLPSAPLHVLLYRFFGWE
DTMPSFAHLSLLLKPEGNGKLSKRDGDRLGFPVFPLEWHDPKTGDVSSGYRESGYFPEAVVNFLALLGWNPGNDQEVMSM
DDLIRLFDLSRCSKSGAKFDYEKGRWFNHHYLLEKSNAEIADLFLPIVESHGIQTTHAYVEKVVGMMKGRVNFVSELWDL
CSFFFIAPTEYDEKTRKKRWKEDSAVQLGEFIEQLRAREPFDVEGTENECKAWIESKGYHLGNIMNAARLALVGEGKGPG
IFDITEALGKEESIRRIQRAIEILK
>Mature_504_residues
TQRKVRVRFAPSPTGALHIGGVRTALYNYLFAKQNGGDMILRIEDTDSQRFVPGAEDYIIEALTWLGIKFDEGVSFGGNY
GPYRQSERREIYKKYVDQLLNDGHAYIAFDTPAELEEKRKEIANFQYDASTRSQMRNSLTLSQEEVQSLIESGHQYVVRA
KIEPNEDIHVNDLIRGEVVINSSILDDKVLYKSADQLPTYHLANIVDDHLMEVTHVIRGEEWLPSAPLHVLLYRFFGWED
TMPSFAHLSLLLKPEGNGKLSKRDGDRLGFPVFPLEWHDPKTGDVSSGYRESGYFPEAVVNFLALLGWNPGNDQEVMSMD
DLIRLFDLSRCSKSGAKFDYEKGRWFNHHYLLEKSNAEIADLFLPIVESHGIQTTHAYVEKVVGMMKGRVNFVSELWDLC
SFFFIAPTEYDEKTRKKRWKEDSAVQLGEFIEQLRAREPFDVEGTENECKAWIESKGYHLGNIMNAARLALVGEGKGPGI
FDITEALGKEESIRRIQRAIEILK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=518, Percent_Identity=34.9420849420849, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI62241042, Length=219, Percent_Identity=27.8538812785388, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1788743, Length=505, Percent_Identity=31.0891089108911, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=29.4339622641509, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786895, Length=342, Percent_Identity=23.3918128654971, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=516, Percent_Identity=35.6589147286822, Blast_Score=263, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=519, Percent_Identity=35.8381502890173, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6321192, Length=315, Percent_Identity=28.8888888888889, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24665321, Length=512, Percent_Identity=35.15625, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24649466, Length=226, Percent_Identity=31.858407079646, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PARD8 (A6LEA1)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303637.1
- ProteinModelPortal:   A6LEA1
- SMR:   A6LEA1
- STRING:   A6LEA1
- GeneID:   5307438
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2289
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   PDIS435591:BDI_2289-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57732; Mature: 57601

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRKVRVRFAPSPTGALHIGGVRTALYNYLFAKQNGGDMILRIEDTDSQRFVPGAEDYI
CCCCEEEEEECCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCHHHHH
IEALTWLGIKFDEGVSFGGNYGPYRQSERREIYKKYVDQLLNDGHAYIAFDTPAELEEKR
HHHHHHHCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
KEIANFQYDASTRSQMRNSLTLSQEEVQSLIESGHQYVVRAKIEPNEDIHVNDLIRGEVV
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEHHHEEECEEE
INSSILDDKVLYKSADQLPTYHLANIVDDHLMEVTHVIRGEEWLPSAPLHVLLYRFFGWE
EECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
DTMPSFAHLSLLLKPEGNGKLSKRDGDRLGFPVFPLEWHDPKTGDVSSGYRESGYFPEAV
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VNFLALLGWNPGNDQEVMSMDDLIRLFDLSRCSKSGAKFDYEKGRWFNHHYLLEKSNAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEEEEECCCCHH
ADLFLPIVESHGIQTTHAYVEKVVGMMKGRVNFVSELWDLCSFFFIAPTEYDEKTRKKRW
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHC
KEDSAVQLGEFIEQLRAREPFDVEGTENECKAWIESKGYHLGNIMNAARLALVGEGKGPG
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCC
IFDITEALGKEESIRRIQRAIEILK
HHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQRKVRVRFAPSPTGALHIGGVRTALYNYLFAKQNGGDMILRIEDTDSQRFVPGAEDYI
CCCEEEEEECCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCHHHHH
IEALTWLGIKFDEGVSFGGNYGPYRQSERREIYKKYVDQLLNDGHAYIAFDTPAELEEKR
HHHHHHHCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
KEIANFQYDASTRSQMRNSLTLSQEEVQSLIESGHQYVVRAKIEPNEDIHVNDLIRGEVV
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEHHHEEECEEE
INSSILDDKVLYKSADQLPTYHLANIVDDHLMEVTHVIRGEEWLPSAPLHVLLYRFFGWE
EECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
DTMPSFAHLSLLLKPEGNGKLSKRDGDRLGFPVFPLEWHDPKTGDVSSGYRESGYFPEAV
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VNFLALLGWNPGNDQEVMSMDDLIRLFDLSRCSKSGAKFDYEKGRWFNHHYLLEKSNAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEEEEECCCCHH
ADLFLPIVESHGIQTTHAYVEKVVGMMKGRVNFVSELWDLCSFFFIAPTEYDEKTRKKRW
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHC
KEDSAVQLGEFIEQLRAREPFDVEGTENECKAWIESKGYHLGNIMNAARLALVGEGKGPG
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCC
IFDITEALGKEESIRRIQRAIEILK
HHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA