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The map label for this gene is waaA [H]

Identifier: 150008893

GI number: 150008893

Start: 2678545

End: 2679780

Strand: Reverse

Name: waaA [H]

Synonym: BDI_2288

Alternate gene names: 150008893

Gene position: 2679780-2678545 (Counterclockwise)

Preceding gene: 150008894

Following gene: 150008886

Centisome position: 55.7

GC content: 48.87

Gene sequence:

>1236_bases
ATGTACAGTTTAGCAATACATCTCTACGCTTTTGTAGTCGCTTTGATCTCGCCTTTCCATAAAAAGGCGAGACTGATGCG
ATTCGGGCAATGGAAAACAAATTCCATACTTCGTGAGAAAATCGACCGGAACGCTAAATACATCTGGTTCCATGCCTCCT
CGTTGGGTGAATTCGAGCAAGGGCGCCCTATGATCGAGAATATCAAGGCGGAACATCCGGAGTATAAGGTGTTGCTGACT
TTCTTTTCTCCCTCCGGTTATGAGGTACGGAAGAATTACAAGGGAGCGGACGTGATCTGCTACCTTCCTTTTGATACGCC
TTTCCGGGTGAAGAAGTTCCTGAATCTGGCGAATCCGGCGATCGCCATATTTATCAAGTACGAGTTTTGGGGGAACTATT
TGCGTGAGTTGCGGAAGCGGGGAATACCGGTTTATATCATTTCCGCTATATTCCGCCCCGACCAGCTATTTTTCCAATGG
TTCGGAAAACCGTACCGGAAGATGCTATCTTATTTTAATCATCTTTTTGTTCAGGACGAGCGTTCGATGAAACTCTTGAA
CGAGTTCGGTATCACGAATGTGACGGTGACCGGCGATACCCGTTTTGATCGGGTGTTGGATGTCCGTAAGCAAGCTCGTG
AGCTTCCGTTTATCGAACGTTTCTTGGAAAGCAAGGAGGGTAAGAGGCCGATCGTGATGGTGGCCGGCAGTTCATGGCCT
CAAGACGAGGCGATCTTTATCCCTTATTTCCATGAGCATCCGGAGATGAAGTTGATTATCGCCCCGCATGAGATTCACCG
GGAACATCTCCTTTCTATCGAGGCGATGCTAAAACGTCCGTCCGTCCGTCTATCGGAGGCGCATGAGGATGATCTGGCCG
ATAAGGATTGCTTGATCATCGACAGTTTCGGACTCTTATCTTCCATTTATCGCTATGGACAGATCGCTTATATCGGTGGT
GGTTTCGGAGCGGGTATCCACAACACGTTGGAAGCCGCCGTTTATGGGATGCCGGTCTTATTCGGCCCGAGATACCATAA
GTTTAAGGAGGCGAAGGATCTGATCGCCGTAGGTGGTGGTTTCTCCATCACAGACGATTCCTCTTTCCGAACGAAGATGG
ACGAGTTATTGACCGACCCGACAGCCTTGGAAACCTCCGGGCAAGCTGCCGGTGATTTTGTGAAAAACAGTGTAGGAGCG
ACGGATCAAATCTTGCGGCAAATCCATATCCGTTAA

Upstream 100 bases:

>100_bases
GCGAGGGCAAGGGCCCCGGCATCTTTGATATTACCGAGGCTTTAGGTAAAGAGGAGTCTATCCGCCGTATCCAACGGGCT
ATCGAGATATTAAAATAATC

Downstream 100 bases:

>100_bases
TAGATAAAGCCGGGTTTGCTATTCGAGTGATTGTATGCCCAGACTCTTTTGATATAGCAATGGAATACGGATGAGATAGA
TGTACGGATTGGTAATTGAA

Product: glycosyl transferase family protein

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MYSLAIHLYAFVVALISPFHKKARLMRFGQWKTNSILREKIDRNAKYIWFHASSLGEFEQGRPMIENIKAEHPEYKVLLT
FFSPSGYEVRKNYKGADVICYLPFDTPFRVKKFLNLANPAIAIFIKYEFWGNYLRELRKRGIPVYIISAIFRPDQLFFQW
FGKPYRKMLSYFNHLFVQDERSMKLLNEFGITNVTVTGDTRFDRVLDVRKQARELPFIERFLESKEGKRPIVMVAGSSWP
QDEAIFIPYFHEHPEMKLIIAPHEIHREHLLSIEAMLKRPSVRLSEAHEDDLADKDCLIIDSFGLLSSIYRYGQIAYIGG
GFGAGIHNTLEAAVYGMPVLFGPRYHKFKEAKDLIAVGGGFSITDDSSFRTKMDELLTDPTALETSGQAAGDFVKNSVGA
TDQILRQIHIR

Sequences:

>Translated_411_residues
MYSLAIHLYAFVVALISPFHKKARLMRFGQWKTNSILREKIDRNAKYIWFHASSLGEFEQGRPMIENIKAEHPEYKVLLT
FFSPSGYEVRKNYKGADVICYLPFDTPFRVKKFLNLANPAIAIFIKYEFWGNYLRELRKRGIPVYIISAIFRPDQLFFQW
FGKPYRKMLSYFNHLFVQDERSMKLLNEFGITNVTVTGDTRFDRVLDVRKQARELPFIERFLESKEGKRPIVMVAGSSWP
QDEAIFIPYFHEHPEMKLIIAPHEIHREHLLSIEAMLKRPSVRLSEAHEDDLADKDCLIIDSFGLLSSIYRYGQIAYIGG
GFGAGIHNTLEAAVYGMPVLFGPRYHKFKEAKDLIAVGGGFSITDDSSFRTKMDELLTDPTALETSGQAAGDFVKNSVGA
TDQILRQIHIR
>Mature_411_residues
MYSLAIHLYAFVVALISPFHKKARLMRFGQWKTNSILREKIDRNAKYIWFHASSLGEFEQGRPMIENIKAEHPEYKVLLT
FFSPSGYEVRKNYKGADVICYLPFDTPFRVKKFLNLANPAIAIFIKYEFWGNYLRELRKRGIPVYIISAIFRPDQLFFQW
FGKPYRKMLSYFNHLFVQDERSMKLLNEFGITNVTVTGDTRFDRVLDVRKQARELPFIERFLESKEGKRPIVMVAGSSWP
QDEAIFIPYFHEHPEMKLIIAPHEIHREHLLSIEAMLKRPSVRLSEAHEDDLADKDCLIIDSFGLLSSIYRYGQIAYIGG
GFGAGIHNTLEAAVYGMPVLFGPRYHKFKEAKDLIAVGGGFSITDDSSFRTKMDELLTDPTALETSGQAAGDFVKNSVGA
TDQILRQIHIR

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=371, Percent_Identity=25.8760107816712, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47426; Mature: 47426

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLAIHLYAFVVALISPFHKKARLMRFGQWKTNSILREKIDRNAKYIWFHASSLGEFEQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHC
GRPMIENIKAEHPEYKVLLTFFSPSGYEVRKNYKGADVICYLPFDTPFRVKKFLNLANPA
CCCHHHHHCCCCCCEEEEEEEECCCCCHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCE
IAIFIKYEFWGNYLRELRKRGIPVYIISAIFRPDQLFFQWFGKPYRKMLSYFNHLFVQDE
EEEEEEEHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHCHHHHHHHHHHHHHEECCH
RSMKLLNEFGITNVTVTGDTRFDRVLDVRKQARELPFIERFLESKEGKRPIVMVAGSSWP
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCC
QDEAIFIPYFHEHPEMKLIIAPHEIHREHLLSIEAMLKRPSVRLSEAHEDDLADKDCLII
CCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEE
DSFGLLSSIYRYGQIAYIGGGFGAGIHNTLEAAVYGMPVLFGPRYHKFKEAKDLIAVGGG
ECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHEEEECCC
FSITDDSSFRTKMDELLTDPTALETSGQAAGDFVKNSVGATDQILRQIHIR
EEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MYSLAIHLYAFVVALISPFHKKARLMRFGQWKTNSILREKIDRNAKYIWFHASSLGEFEQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHC
GRPMIENIKAEHPEYKVLLTFFSPSGYEVRKNYKGADVICYLPFDTPFRVKKFLNLANPA
CCCHHHHHCCCCCCEEEEEEEECCCCCHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCE
IAIFIKYEFWGNYLRELRKRGIPVYIISAIFRPDQLFFQWFGKPYRKMLSYFNHLFVQDE
EEEEEEEHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHCHHHHHHHHHHHHHEECCH
RSMKLLNEFGITNVTVTGDTRFDRVLDVRKQARELPFIERFLESKEGKRPIVMVAGSSWP
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCC
QDEAIFIPYFHEHPEMKLIIAPHEIHREHLLSIEAMLKRPSVRLSEAHEDDLADKDCLII
CCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEE
DSFGLLSSIYRYGQIAYIGGGFGAGIHNTLEAAVYGMPVLFGPRYHKFKEAKDLIAVGGG
ECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHEEEECCC
FSITDDSSFRTKMDELLTDPTALETSGQAAGDFVKNSVGATDQILRQIHIR
EEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]