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The map label for this gene is proB [H]

Identifier: 150008866

GI number: 150008866

Start: 2646927

End: 2648000

Strand: Direct

Name: proB [H]

Synonym: BDI_2256

Alternate gene names: 150008866

Gene position: 2646927-2648000 (Clockwise)

Preceding gene: 150008865

Following gene: 150008867

Centisome position: 55.01

GC content: 48.32

Gene sequence:

>1074_bases
TTGAAAAGGATCGCTGTAAAAATAGGAAGCAATGTCCTGACCCGCAAGGATGGGACGCTAGATATCACCCGCATGTCCGC
GTTGGTTGACCAAGTAGCGGACTTGCACCGGCAAGGTATGGAGATCGTCTTGATCTCCTCCGGTGCCGTGGCTTCTGGAC
GAAGCGAGATAAAAGCCGGCAAAAAGCTGGATCCCGTATCAGCCCGGCAACTGTATTCGGCGGTAGGACAGGCCAAGTTG
ATCAACCGGTATTACGAGTTATTCCGGGAGCATGGCATGACTTGCGGGCAAGTGCTTACTACAAAAGAGAATTTCGGGAG
TCGTACGCATTACCTGAACCAGAAGCATTGCATGGAGGTAATGCTTGAAAACAAGGTGATCCCCATCGTAAATGAGAATG
ATACGATATCCGTAACCGAGCTGATGTTCACGGATAATGATGAGTTATCCGGCTTGATCGCTACGATGATGGGAATGGAT
GCCTTGGTGATTCTCAGTAATATAGACGGGATTTACAATGGAAATCCGTCGGACCCGGATAGTTCCGTGATCCGGGAAAT
CGAATGTGGGAAAACAGATTTGTCGGAATATGTACAGACTAGCAAATCATCCTTTGGACGGGGAGGTATGTTGACGAAAT
GTCATATCGCCCAAAAAGTGGCGGACGAAGGTATTGCGGTTATCATCGCCAACGGCAAAAAGGATAATATCCTTCCGATG
TTATTGGCGGTGGATAGCGACACGGTCTGCACCCGTTTCATTCCGGCCTCGAAACCCGTCAGCAGCGTAAAGAAATGGAT
CGCCCACTCCGAGGGTTTCGCCAAAGGGGAAATACATATCAATAAAGGAGCCGAGGAGGCATTGACCGCCCCGAAAGCGA
CTAGCGTATTATTGGTTGGAGTAACCCGCATCGAGGGTGATTTCGAAAAAGATGATATCGTAAAGATAATGAATGAGGAA
GGCGTGCAAATCGGCGTAGGCTGTACCGCCTATGACAGCGAGGAAGCACGTAAGCAAATAGGGCAACGGGATTTAAAACC
GCTTATACATTATGATTATCTATATCTGGATTAA

Upstream 100 bases:

>100_bases
TGTTGTCGAATTCGTGGACGAACTGCCGAAAACAATCAGCGGAAAGATCCGACGGGTAGAGATCCGGGATAAAGATAATA
AATAATAAATCATAACTTTC

Downstream 100 bases:

>100_bases
AAAGTATGAATGTAAAAGATATGCTGCGACAGGCATCGGATGCGGCTAAACAATTGATAACGCTATCGGATCAAACGATT
ATCGGGATCTTAAAGGAGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKRIAVKIGSNVLTRKDGTLDITRMSALVDQVADLHRQGMEIVLISSGAVASGRSEIKAGKKLDPVSARQLYSAVGQAKL
INRYYELFREHGMTCGQVLTTKENFGSRTHYLNQKHCMEVMLENKVIPIVNENDTISVTELMFTDNDELSGLIATMMGMD
ALVILSNIDGIYNGNPSDPDSSVIREIECGKTDLSEYVQTSKSSFGRGGMLTKCHIAQKVADEGIAVIIANGKKDNILPM
LLAVDSDTVCTRFIPASKPVSSVKKWIAHSEGFAKGEIHINKGAEEALTAPKATSVLLVGVTRIEGDFEKDDIVKIMNEE
GVQIGVGCTAYDSEEARKQIGQRDLKPLIHYDYLYLD

Sequences:

>Translated_357_residues
MKRIAVKIGSNVLTRKDGTLDITRMSALVDQVADLHRQGMEIVLISSGAVASGRSEIKAGKKLDPVSARQLYSAVGQAKL
INRYYELFREHGMTCGQVLTTKENFGSRTHYLNQKHCMEVMLENKVIPIVNENDTISVTELMFTDNDELSGLIATMMGMD
ALVILSNIDGIYNGNPSDPDSSVIREIECGKTDLSEYVQTSKSSFGRGGMLTKCHIAQKVADEGIAVIIANGKKDNILPM
LLAVDSDTVCTRFIPASKPVSSVKKWIAHSEGFAKGEIHINKGAEEALTAPKATSVLLVGVTRIEGDFEKDDIVKIMNEE
GVQIGVGCTAYDSEEARKQIGQRDLKPLIHYDYLYLD
>Mature_357_residues
MKRIAVKIGSNVLTRKDGTLDITRMSALVDQVADLHRQGMEIVLISSGAVASGRSEIKAGKKLDPVSARQLYSAVGQAKL
INRYYELFREHGMTCGQVLTTKENFGSRTHYLNQKHCMEVMLENKVIPIVNENDTISVTELMFTDNDELSGLIATMMGMD
ALVILSNIDGIYNGNPSDPDSSVIREIECGKTDLSEYVQTSKSSFGRGGMLTKCHIAQKVADEGIAVIIANGKKDNILPM
LLAVDSDTVCTRFIPASKPVSSVKKWIAHSEGFAKGEIHINKGAEEALTAPKATSVLLVGVTRIEGDFEKDDIVKIMNEE
GVQIGVGCTAYDSEEARKQIGQRDLKPLIHYDYLYLD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=267, Percent_Identity=29.5880149812734, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI62912457, Length=265, Percent_Identity=29.4339622641509, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786437, Length=353, Percent_Identity=31.728045325779, Blast_Score=198, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17569967, Length=248, Percent_Identity=30.6451612903226, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=248, Percent_Identity=30.6451612903226, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321822, Length=262, Percent_Identity=33.969465648855, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320506, Length=356, Percent_Identity=29.4943820224719, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21357643, Length=298, Percent_Identity=28.1879194630872, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39102; Mature: 39102

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVKIGSNVLTRKDGTLDITRMSALVDQVADLHRQGMEIVLISSGAVASGRSEIKAG
CCEEEEEECCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCC
KKLDPVSARQLYSAVGQAKLINRYYELFREHGMTCGQVLTTKENFGSRTHYLNQKHCMEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHCCCHHHHCCHHHHHHH
MLENKVIPIVNENDTISVTELMFTDNDELSGLIATMMGMDALVILSNIDGIYNGNPSDPD
HHCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCH
SSVIREIECGKTDLSEYVQTSKSSFGRGGMLTKCHIAQKVADEGIAVIIANGKKDNILPM
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LLAVDSDTVCTRFIPASKPVSSVKKWIAHSEGFAKGEIHINKGAEEALTAPKATSVLLVG
EEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCEEEEEE
VTRIEGDFEKDDIVKIMNEEGVQIGVGCTAYDSEEARKQIGQRDLKPLIHYDYLYLD
EEECCCCCCHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHHCCHHHEEEEEEEC
>Mature Secondary Structure
MKRIAVKIGSNVLTRKDGTLDITRMSALVDQVADLHRQGMEIVLISSGAVASGRSEIKAG
CCEEEEEECCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCC
KKLDPVSARQLYSAVGQAKLINRYYELFREHGMTCGQVLTTKENFGSRTHYLNQKHCMEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHCCCHHHHCCHHHHHHH
MLENKVIPIVNENDTISVTELMFTDNDELSGLIATMMGMDALVILSNIDGIYNGNPSDPD
HHCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCH
SSVIREIECGKTDLSEYVQTSKSSFGRGGMLTKCHIAQKVADEGIAVIIANGKKDNILPM
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LLAVDSDTVCTRFIPASKPVSSVKKWIAHSEGFAKGEIHINKGAEEALTAPKATSVLLVG
EEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCEEEEEE
VTRIEGDFEKDDIVKIMNEEGVQIGVGCTAYDSEEARKQIGQRDLKPLIHYDYLYLD
EEECCCCCCHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHHCCHHHEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]