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The map label for this gene is proA

Identifier: 150008867

GI number: 150008867

Start: 2648006

End: 2649253

Strand: Direct

Name: proA

Synonym: BDI_2257

Alternate gene names: 150008867

Gene position: 2648006-2649253 (Clockwise)

Preceding gene: 150008866

Following gene: 150008868

Centisome position: 55.04

GC content: 48.4

Gene sequence:

>1248_bases
ATGAATGTAAAAGATATGCTGCGACAGGCATCGGATGCGGCTAAACAATTGATAACGCTATCGGATCAAACGATTATCGG
GATCTTAAAGGAGACAGCGCAGGTTCTACGGACTCATACGGAGGAGGTACTCGCCGCGAATCGCCAAGATCTGGAACGTA
TGGACCCAGCGAACCCGAAATACGACCGGCTGAAATTAACAGAGGAAAGGCTACAAGGTATCGCCGCCGATATGGAGAAT
GTATCGACATTACCTTCTCCATTAAATAAGGTCTTGAGTGAGACTATCCGCCCGAACGGAATGGTTATCCGAAAAGTAAC
CGTGCCTTTCGGGGTGATCGGCGTGATCTATGAGGCTCGTCCGAATGTTACCTTCGATGTTTTTTCCTTATGTTTCCGAA
GCGGTAATGCTTGCGTACTAAAGGGTGGATCGGATGCGGACTTCTCTAACCGAGCCTTGGTACGCATCATCCATTCTGTA
TTGGAGCGTCAGGGTATAAATCCGGCTGTATGCACTTTACTACCTCCGGATCGAGAGGCTACGGCGGAATTACTGGGAGC
GGTCGGCTTGGTTGACTTAATCATTCCTCGTGGAAGCAGTTCCTTGATTCACTTTGTCCGGGAACATGCGAAAGTTCCGG
TGATCGAGACTGGCGCTGGTATCTGTCATACCTATTTTGATCGCTCCGGAGATAAAGGCAAGGGACGTGAGATCGTAAAT
AACGCAAAGACACGGAGGGTCAGCGTATGTAATGCCTTGGATTGCTTAATCATCCACCGAGAACGTCTATCTGATCTTCC
GTATATCTGTGAAAAGCTTCCGGACAGCAACGTAATCATTTATGCGGACGAACCGGCTTATGCCGCTCTATCCGAACATT
ATCCGGAGACGCTTTTACAACAGGCTAACGAAAACAGTTTCGGTACCGAGTTCCTCGATTACAAAATGAGTATCCGTACC
GTATCCTCTTTAGACGAGGTTCTATCACATATCGCCCGCTACAGTTCAAAACATAGCGAAAGCATAATCAGCGAAGATCC
GGAAACTATCCGGCGCTTCCAGCAATTGGTAGATGCCGCTTGCGTATACGCAAATGTATCTACCGCCTTTACTGATGGGG
CCCAATTCGGATTCGGGGCGGAGATCGGAATCAGTACCCAAAAGCTACATGCCCGGGGACCGATGGCGCTTCCTGAATTA
ACGACTTATAAATACATCATAGAAGGAGACGGGCAGACGAGAATATAG

Upstream 100 bases:

>100_bases
GCTGTACCGCCTATGACAGCGAGGAAGCACGTAAGCAAATAGGGCAACGGGATTTAAAACCGCTTATACATTATGATTAT
CTATATCTGGATTAAAAAGT

Downstream 100 bases:

>100_bases
GAACTCGCCTGTCTCTTTCTTCTTGAACAACACTTAAATCTAAACGAATAATGTCAACAGTAATAGATTCAAATATTTTC
AACACTTTAGTTTCAAATAC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVKDMLRQASDAAKQLITLSDQTIIGILKETAQVLRTHTEEVLAANRQDLERMDPANPKYDRLKLTEERLQGIAADMEN
VSTLPSPLNKVLSETIRPNGMVIRKVTVPFGVIGVIYEARPNVTFDVFSLCFRSGNACVLKGGSDADFSNRALVRIIHSV
LERQGINPAVCTLLPPDREATAELLGAVGLVDLIIPRGSSSLIHFVREHAKVPVIETGAGICHTYFDRSGDKGKGREIVN
NAKTRRVSVCNALDCLIIHRERLSDLPYICEKLPDSNVIIYADEPAYAALSEHYPETLLQQANENSFGTEFLDYKMSIRT
VSSLDEVLSHIARYSSKHSESIISEDPETIRRFQQLVDAACVYANVSTAFTDGAQFGFGAEIGISTQKLHARGPMALPEL
TTYKYIIEGDGQTRI

Sequences:

>Translated_415_residues
MNVKDMLRQASDAAKQLITLSDQTIIGILKETAQVLRTHTEEVLAANRQDLERMDPANPKYDRLKLTEERLQGIAADMEN
VSTLPSPLNKVLSETIRPNGMVIRKVTVPFGVIGVIYEARPNVTFDVFSLCFRSGNACVLKGGSDADFSNRALVRIIHSV
LERQGINPAVCTLLPPDREATAELLGAVGLVDLIIPRGSSSLIHFVREHAKVPVIETGAGICHTYFDRSGDKGKGREIVN
NAKTRRVSVCNALDCLIIHRERLSDLPYICEKLPDSNVIIYADEPAYAALSEHYPETLLQQANENSFGTEFLDYKMSIRT
VSSLDEVLSHIARYSSKHSESIISEDPETIRRFQQLVDAACVYANVSTAFTDGAQFGFGAEIGISTQKLHARGPMALPEL
TTYKYIIEGDGQTRI
>Mature_415_residues
MNVKDMLRQASDAAKQLITLSDQTIIGILKETAQVLRTHTEEVLAANRQDLERMDPANPKYDRLKLTEERLQGIAADMEN
VSTLPSPLNKVLSETIRPNGMVIRKVTVPFGVIGVIYEARPNVTFDVFSLCFRSGNACVLKGGSDADFSNRALVRIIHSV
LERQGINPAVCTLLPPDREATAELLGAVGLVDLIIPRGSSSLIHFVREHAKVPVIETGAGICHTYFDRSGDKGKGREIVN
NAKTRRVSVCNALDCLIIHRERLSDLPYICEKLPDSNVIIYADEPAYAALSEHYPETLLQQANENSFGTEFLDYKMSIRT
VSSLDEVLSHIARYSSKHSESIISEDPETIRRFQQLVDAACVYANVSTAFTDGAQFGFGAEIGISTQKLHARGPMALPEL
TTYKYIIEGDGQTRI

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=406, Percent_Identity=35.4679802955665, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI62912457, Length=406, Percent_Identity=35.4679802955665, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786438, Length=419, Percent_Identity=39.8568019093079, Blast_Score=291, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17569967, Length=418, Percent_Identity=35.1674641148325, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17569969, Length=418, Percent_Identity=35.1674641148325, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=32.7102803738318, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21357643, Length=414, Percent_Identity=33.5748792270531, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_PARD8 (A6LE74)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303610.1
- ProteinModelPortal:   A6LE74
- SMR:   A6LE74
- STRING:   A6LE74
- GeneID:   5307406
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2257
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- BioCyc:   PDIS435591:BDI_2257-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR000965
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45818; Mature: 45818

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKDMLRQASDAAKQLITLSDQTIIGILKETAQVLRTHTEEVLAANRQDLERMDPANPK
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
YDRLKLTEERLQGIAADMENVSTLPSPLNKVLSETIRPNGMVIRKVTVPFGVIGVIYEAR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHEEECC
PNVTFDVFSLCFRSGNACVLKGGSDADFSNRALVRIIHSVLERQGINPAVCTLLPPDREA
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
TAELLGAVGLVDLIIPRGSSSLIHFVREHAKVPVIETGAGICHTYFDRSGDKGKGREIVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCHHHHH
NAKTRRVSVCNALDCLIIHRERLSDLPYICEKLPDSNVIIYADEPAYAALSEHYPETLLQ
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
QANENSFGTEFLDYKMSIRTVSSLDEVLSHIARYSSKHSESIISEDPETIRRFQQLVDAA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CVYANVSTAFTDGAQFGFGAEIGISTQKLHARGPMALPELTTYKYIIEGDGQTRI
HHHHCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MNVKDMLRQASDAAKQLITLSDQTIIGILKETAQVLRTHTEEVLAANRQDLERMDPANPK
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
YDRLKLTEERLQGIAADMENVSTLPSPLNKVLSETIRPNGMVIRKVTVPFGVIGVIYEAR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHEEECC
PNVTFDVFSLCFRSGNACVLKGGSDADFSNRALVRIIHSVLERQGINPAVCTLLPPDREA
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
TAELLGAVGLVDLIIPRGSSSLIHFVREHAKVPVIETGAGICHTYFDRSGDKGKGREIVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCHHHHH
NAKTRRVSVCNALDCLIIHRERLSDLPYICEKLPDSNVIIYADEPAYAALSEHYPETLLQ
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
QANENSFGTEFLDYKMSIRTVSSLDEVLSHIARYSSKHSESIISEDPETIRRFQQLVDAA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CVYANVSTAFTDGAQFGFGAEIGISTQKLHARGPMALPELTTYKYIIEGDGQTRI
HHHHCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA