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The map label for this gene is lipA

Identifier: 150008802

GI number: 150008802

Start: 2579484

End: 2580329

Strand: Reverse

Name: lipA

Synonym: BDI_2192

Alternate gene names: 150008802

Gene position: 2580329-2579484 (Counterclockwise)

Preceding gene: 150008803

Following gene: 150008801

Centisome position: 53.63

GC content: 47.99

Gene sequence:

>846_bases
ATGGCGCAGCATTTAAGAAAGCCGGATTGGTTGAAGATCCGGCTTGGCGGAAATGAGCAATTCACGAAGACAAAAAGTAT
TGTTGAATCTCATTGCCTTCACACAATATGCACAAGTGGTAAATGCCCGAATATGGGTGAATGTTGGAGCCGGGGAACAG
CTACATTCATGATCGGTGGTGAGATCTGTACTCGCTCTTGTCGTTTTTGTAATACGCTAACGGGTAAACCTCTTCCGTTG
GACCCGAAAGAGCCTGCGAATGTAGCGGAGAGCATCCGTTTAATGAATCTCAAGCATGCGGTGATCACGTCCGTTGATCG
TGATGACTTACCAGACTTAGGCGCTTCTCATTGGGTAAATACGATCCGTACGATCAAAGAGGTGAATCCTCAAACGACCG
TGGAAGTCTTAATCCCGGATTTTCAAGGACGATTGGATCTGGTTGATCAGGTCGTGGATGCGGCGCCTGAGATTATCTCC
CATAATATGGAGACTGTACGCCGTATCAGTCCGCAAGTGCGTAGCGCCGCTAAGTATGACGTGAGCTTATCCGTTTTGCG
ACGAATCGCTGAGCGGGGTGTGGTAGCCAAGACTGGTATCATGGTCGGACTGGGGGAGACTGAGGATGAGGTGCTTGAAT
TAATGGATGATGTTTTGCAGGCGGGTGTTTCTGTTTTGACAATCGGGCAGTATTTACAGCCCTCTCGCAAGAATATTCCC
GTCTCTGAATATGTGACTCCTGAACGCTTTGAATATTATAGACAACAGGCCGTTAACAAAGGTTTTAAGAAAGTAGAGAG
CGCTCCGCTGGTGCGCTCGTCGTACCATGCGGAAAAGCATATATGA

Upstream 100 bases:

>100_bases
GCATGTTTATAAAGATCGGAATCATAGCATATATGGTGGTAATACCCGTTACCATTTGTATACGAAGATGGCTAATTATT
TATTCAATAATTTGTAAGAC

Downstream 100 bases:

>100_bases
TTCATGAACTTTTGACAATCTTTGTTGTTATTAATATAAAAGTGGACTAAAGGGGGGGAAGGAATGAACAGAGTAAAGAA
AGGTCTAGATAATCAGGCGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAQHLRKPDWLKIRLGGNEQFTKTKSIVESHCLHTICTSGKCPNMGECWSRGTATFMIGGEICTRSCRFCNTLTGKPLPL
DPKEPANVAESIRLMNLKHAVITSVDRDDLPDLGASHWVNTIRTIKEVNPQTTVEVLIPDFQGRLDLVDQVVDAAPEIIS
HNMETVRRISPQVRSAAKYDVSLSVLRRIAERGVVAKTGIMVGLGETEDEVLELMDDVLQAGVSVLTIGQYLQPSRKNIP
VSEYVTPERFEYYRQQAVNKGFKKVESAPLVRSSYHAEKHI

Sequences:

>Translated_281_residues
MAQHLRKPDWLKIRLGGNEQFTKTKSIVESHCLHTICTSGKCPNMGECWSRGTATFMIGGEICTRSCRFCNTLTGKPLPL
DPKEPANVAESIRLMNLKHAVITSVDRDDLPDLGASHWVNTIRTIKEVNPQTTVEVLIPDFQGRLDLVDQVVDAAPEIIS
HNMETVRRISPQVRSAAKYDVSLSVLRRIAERGVVAKTGIMVGLGETEDEVLELMDDVLQAGVSVLTIGQYLQPSRKNIP
VSEYVTPERFEYYRQQAVNKGFKKVESAPLVRSSYHAEKHI
>Mature_280_residues
AQHLRKPDWLKIRLGGNEQFTKTKSIVESHCLHTICTSGKCPNMGECWSRGTATFMIGGEICTRSCRFCNTLTGKPLPLD
PKEPANVAESIRLMNLKHAVITSVDRDDLPDLGASHWVNTIRTIKEVNPQTTVEVLIPDFQGRLDLVDQVVDAAPEIISH
NMETVRRISPQVRSAAKYDVSLSVLRRIAERGVVAKTGIMVGLGETEDEVLELMDDVLQAGVSVLTIGQYLQPSRKNIPV
SEYVTPERFEYYRQQAVNKGFKKVESAPLVRSSYHAEKHI

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=43.1578947368421, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=42, Blast_Score=209, Evalue=2e-54,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=46.0431654676259, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.1602787456446, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=43.010752688172, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=43.859649122807, Blast_Score=243, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PARD8 (A6LE09)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303545.1
- ProteinModelPortal:   A6LE09
- STRING:   A6LE09
- GeneID:   5307341
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2192
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   DPHEPQR
- ProtClustDB:   PRK05481
- BioCyc:   PDIS435591:BDI_2192-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 31394; Mature: 31263

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHLRKPDWLKIRLGGNEQFTKTKSIVESHCLHTICTSGKCPNMGECWSRGTATFMIGG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECH
EICTRSCRFCNTLTGKPLPLDPKEPANVAESIRLMNLKHAVITSVDRDDLPDLGASHWVN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
TIRTIKEVNPQTTVEVLIPDFQGRLDLVDQVVDAAPEIISHNMETVRRISPQVRSAAKYD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHH
VSLSVLRRIAERGVVAKTGIMVGLGETEDEVLELMDDVLQAGVSVLTIGQYLQPSRKNIP
HHHHHHHHHHHCCCEECCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
VSEYVTPERFEYYRQQAVNKGFKKVESAPLVRSSYHAEKHI
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHCCC
>Mature Secondary Structure 
AQHLRKPDWLKIRLGGNEQFTKTKSIVESHCLHTICTSGKCPNMGECWSRGTATFMIGG
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECH
EICTRSCRFCNTLTGKPLPLDPKEPANVAESIRLMNLKHAVITSVDRDDLPDLGASHWVN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
TIRTIKEVNPQTTVEVLIPDFQGRLDLVDQVVDAAPEIISHNMETVRRISPQVRSAAKYD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHH
VSLSVLRRIAERGVVAKTGIMVGLGETEDEVLELMDDVLQAGVSVLTIGQYLQPSRKNIP
HHHHHHHHHHHCCCEECCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
VSEYVTPERFEYYRQQAVNKGFKKVESAPLVRSSYHAEKHI
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA