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The map label for this gene is cimA [H]

Identifier: 150008671

GI number: 150008671

Start: 2431244

End: 2432758

Strand: Reverse

Name: cimA [H]

Synonym: BDI_2061

Alternate gene names: 150008671

Gene position: 2432758-2431244 (Counterclockwise)

Preceding gene: 150008672

Following gene: 150008670

Centisome position: 50.56

GC content: 52.21

Gene sequence:

>1515_bases
ATGATAGAAATAATGGATACAACCCTCAGAGATGGGGAGCAAACTTCGGGCGTCTCCTTCGCCGCTCACGAGAAGCTTAG
CATAGCGCAAGTGCTGCTGAACGACTTAGGCGTGAACCGGGTGGAGATCGCCTCCGCCCGTGTGTCGGACGGAGAGTTTG
AGGCCGTGAAGCGTGTAGCCGCTTGGGCCGCCCGTTCCGGAAACCTAGACAAATTGGAGGTCTTGGGTTTCGTGGATGGT
ACGGTCTCTCTCGACTGGATTGAATCCGCAGGTTGCCGTGTCGTGAACTTGCTTTGCAAGGGCTCTTATAAGCATGTCAC
CGAACAGCTTCGAAAGACTCCCGAGCAACACGTTGACGATATTCGTACGGTCGTATTGGAAGCGGCGAAACGGAAGATCG
ACGTGAATATCTATTTGGAGGACTGGTCGAACGGTATCAAGCATTCCCCGGATTACGTATTCTTGGTAATCGATGCGTTG
AAAGGACTTCCGATCTGCCGTTTCATGCTCCCGGATACCTTGGGAGTCTTGAATCCCGGAAATACCTACGAGTATTGCAA
GATGATGGTGGACCGTTACCCTGATTTACGGTTTGACTTCCATGCGCATAACGACTATGATCTTGCCGTAGCGAATGTAT
ATTCCGCCATCCGTGCGGGTATCAAGGGAATCCATACGACCGTGAACGGTTTGGGAGAGCGTGCGGGTAATGCTCCATTG
AGCAGCGTCCTTGCCGTCATCAAGGATCAGTTGGGAGAGGAGACGAACTTGAGGGAGGAAAAGATAAACAAGGCCAGTCG
TTTGGTCGAGACCTATTCGGGGGTGCATATCCCGCCCAATAAGCCGATTATCGGAGAGCATGTGTTTACGCAATGCGCCG
GTGTCCATGCGGACGGTGATAGCAAGAACAACCTGTATTGCAACGACCTGCTTCCTGAGCGTTTCGGACGGGTTCGCGAG
TACGCCTTGGGCAAGACCTCCGGAAAAGCGAATATCCGCAAGAATCTGGAAGCGCTCGGTATCGACATGGATGAGGGCTC
GATGCGTAAGGTTACGGAACGGATCATTGAGTTAGGGGATAAGAAAGAAATGGTTACCCCGGAGGATCTTCCTTATATTA
TCAGCGATGTGCTTCATCATGATAATGCGCTGGAGGATCAGAAAATACGGATCTTGAACTATTCCTTATCCTTGGCCCAA
GGGCTGAAACCGGTGGCTACCTTGAAGATCGAGATCAATGGCGAGGCTTACGAGGAATCGGCTTCCGGTGATGGCCAATA
CGACGCTTTCGTTCGTGCCCTACGCCGGATTTATTCCCGTTTGAACCGCCCGTTCCCGATGTTGACGAACTATTCGGTAT
CCATCCCGCCGGGAGGACGTACGGATGCGTTCGTGCAGACGGTCATCAGCTGGAATTATGCCGGGGTCGATTTCAAGACT
CGTGGTCTGGACGCTGATCAGACTGAGGCGGCGATCAAGGCGACATTGAAGATGCTGAACAAGATAGAGGATTAA

Upstream 100 bases:

>100_bases
ACGCCTATAAAAAGGATTGCCTGATCAACGGATTGGACGATATCGACTATTTACTGGCAAATAAATCAAAGATAGAAGCC
TTCGAGGCAGCACGCTGATT

Downstream 100 bases:

>100_bases
TAATTAAAAAGGTAAAGGGTATGAAAAAGTTGACTATCGCGCTTGTAGCGCACGACAATCGTAAGGCGGAAATGGTAGAA
TGGGCGGTACATAACGCTGA

Product: 2-isopropylmalate synthase LeuA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQVLLNDLGVNRVEIASARVSDGEFEAVKRVAAWAARSGNLDKLEVLGFVDG
TVSLDWIESAGCRVVNLLCKGSYKHVTEQLRKTPEQHVDDIRTVVLEAAKRKIDVNIYLEDWSNGIKHSPDYVFLVIDAL
KGLPICRFMLPDTLGVLNPGNTYEYCKMMVDRYPDLRFDFHAHNDYDLAVANVYSAIRAGIKGIHTTVNGLGERAGNAPL
SSVLAVIKDQLGEETNLREEKINKASRLVETYSGVHIPPNKPIIGEHVFTQCAGVHADGDSKNNLYCNDLLPERFGRVRE
YALGKTSGKANIRKNLEALGIDMDEGSMRKVTERIIELGDKKEMVTPEDLPYIISDVLHHDNALEDQKIRILNYSLSLAQ
GLKPVATLKIEINGEAYEESASGDGQYDAFVRALRRIYSRLNRPFPMLTNYSVSIPPGGRTDAFVQTVISWNYAGVDFKT
RGLDADQTEAAIKATLKMLNKIED

Sequences:

>Translated_504_residues
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQVLLNDLGVNRVEIASARVSDGEFEAVKRVAAWAARSGNLDKLEVLGFVDG
TVSLDWIESAGCRVVNLLCKGSYKHVTEQLRKTPEQHVDDIRTVVLEAAKRKIDVNIYLEDWSNGIKHSPDYVFLVIDAL
KGLPICRFMLPDTLGVLNPGNTYEYCKMMVDRYPDLRFDFHAHNDYDLAVANVYSAIRAGIKGIHTTVNGLGERAGNAPL
SSVLAVIKDQLGEETNLREEKINKASRLVETYSGVHIPPNKPIIGEHVFTQCAGVHADGDSKNNLYCNDLLPERFGRVRE
YALGKTSGKANIRKNLEALGIDMDEGSMRKVTERIIELGDKKEMVTPEDLPYIISDVLHHDNALEDQKIRILNYSLSLAQ
GLKPVATLKIEINGEAYEESASGDGQYDAFVRALRRIYSRLNRPFPMLTNYSVSIPPGGRTDAFVQTVISWNYAGVDFKT
RGLDADQTEAAIKATLKMLNKIED
>Mature_504_residues
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQVLLNDLGVNRVEIASARVSDGEFEAVKRVAAWAARSGNLDKLEVLGFVDG
TVSLDWIESAGCRVVNLLCKGSYKHVTEQLRKTPEQHVDDIRTVVLEAAKRKIDVNIYLEDWSNGIKHSPDYVFLVIDAL
KGLPICRFMLPDTLGVLNPGNTYEYCKMMVDRYPDLRFDFHAHNDYDLAVANVYSAIRAGIKGIHTTVNGLGERAGNAPL
SSVLAVIKDQLGEETNLREEKINKASRLVETYSGVHIPPNKPIIGEHVFTQCAGVHADGDSKNNLYCNDLLPERFGRVRE
YALGKTSGKANIRKNLEALGIDMDEGSMRKVTERIIELGDKKEMVTPEDLPYIISDVLHHDNALEDQKIRILNYSLSLAQ
GLKPVATLKIEINGEAYEESASGDGQYDAFVRALRRIYSRLNRPFPMLTNYSVSIPPGGRTDAFVQTVISWNYAGVDFKT
RGLDADQTEAAIKATLKMLNKIED

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=29.0513833992095, Blast_Score=168, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320071, Length=381, Percent_Identity=25.9842519685039, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320019, Length=369, Percent_Identity=25.7452574525745, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324682, Length=364, Percent_Identity=23.9010989010989, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 55984; Mature: 55984

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQVLLNDLGVNRVEIASARVSDGEFEAVKRVA
CEEEECCHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
AWAARSGNLDKLEVLGFVDGTVSLDWIESAGCRVVNLLCKGSYKHVTEQLRKTPEQHVDD
HHHHCCCCCCEEEEEEEECCCEEEHHHHCCCCHHHHHHHCCCHHHHHHHHHHCHHHHHHH
IRTVVLEAAKRKIDVNIYLEDWSNGIKHSPDYVFLVIDALKGLPICRFMLPDTLGVLNPG
HHHHHHHHHHHEEEEEEEEECCCCCCCCCCCEEEEEEHHHCCCCEEHHHCCCHHCCCCCC
NTYEYCKMMVDRYPDLRFDFHAHNDYDLAVANVYSAIRAGIKGIHTTVNGLGERAGNAPL
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SSVLAVIKDQLGEETNLREEKINKASRLVETYSGVHIPPNKPIIGEHVFTQCAGVHADGD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCC
SKNNLYCNDLLPERFGRVREYALGKTSGKANIRKNLEALGIDMDEGSMRKVTERIIELGD
CCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
KKEMVTPEDLPYIISDVLHHDNALEDQKIRILNYSLSLAQGLKPVATLKIEINGEAYEES
CCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEEECCCHHHCC
ASGDGQYDAFVRALRRIYSRLNRPFPMLTNYSVSIPPGGRTDAFVQTVISWNYAGVDFKT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEE
RGLDADQTEAAIKATLKMLNKIED
CCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQVLLNDLGVNRVEIASARVSDGEFEAVKRVA
CEEEECCHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
AWAARSGNLDKLEVLGFVDGTVSLDWIESAGCRVVNLLCKGSYKHVTEQLRKTPEQHVDD
HHHHCCCCCCEEEEEEEECCCEEEHHHHCCCCHHHHHHHCCCHHHHHHHHHHCHHHHHHH
IRTVVLEAAKRKIDVNIYLEDWSNGIKHSPDYVFLVIDALKGLPICRFMLPDTLGVLNPG
HHHHHHHHHHHEEEEEEEEECCCCCCCCCCCEEEEEEHHHCCCCEEHHHCCCHHCCCCCC
NTYEYCKMMVDRYPDLRFDFHAHNDYDLAVANVYSAIRAGIKGIHTTVNGLGERAGNAPL
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SSVLAVIKDQLGEETNLREEKINKASRLVETYSGVHIPPNKPIIGEHVFTQCAGVHADGD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCC
SKNNLYCNDLLPERFGRVREYALGKTSGKANIRKNLEALGIDMDEGSMRKVTERIIELGD
CCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
KKEMVTPEDLPYIISDVLHHDNALEDQKIRILNYSLSLAQGLKPVATLKIEINGEAYEES
CCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEEECCCHHHCC
ASGDGQYDAFVRALRRIYSRLNRPFPMLTNYSVSIPPGGRTDAFVQTVISWNYAGVDFKT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEE
RGLDADQTEAAIKATLKMLNKIED
CCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]