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The map label for this gene is leuD [H]

Identifier: 150008672

GI number: 150008672

Start: 2432761

End: 2433348

Strand: Reverse

Name: leuD [H]

Synonym: BDI_2062

Alternate gene names: 150008672

Gene position: 2433348-2432761 (Counterclockwise)

Preceding gene: 150008673

Following gene: 150008671

Centisome position: 50.57

GC content: 46.43

Gene sequence:

>588_bases
ATGAAAACAAAATTCAATATAATCACAAGCACTTGCGTACCTCTTCCTTTAGAGAACGTGGATACCGACCAGATTATCCC
AGCCCGCTTTCTGAAAGCGACTACCAAAGAAGGGTTCGGCGAGAACTTATTCCGGGATTGGCGATATGATAAGGAAGGTA
ATAAGATCGAATCATTCGTCTTGAACGATCCTACGTATAGCGGAAAGATATTGGTAGCGGGAAAGAACTTCGGTTCCGGT
TCCAGCCGTGAGCATGCGGCTTGGGCGATCGCCGATTACGGTTTCCGTGTCGTGGTCTCCAGTTTCTTCGCCGATATCCA
TAAAAACAACGAGTTGAACAACTTCGTGCTGCCGGTTGTTGTGACTGAAGAGTTCCTCGCTGAATTGTTCGATTCGATTT
ACAAGGACCCGAAAACTGAGGTCGAGGTAAATCTACCGGAGCAAACGATCACGAACAAGGCTACGGGCAAAAGCGAGCAT
TTCGAGATCAACGCCTATAAAAAGGATTGCCTGATCAACGGATTGGACGATATCGACTATTTACTGGCAAATAAATCAAA
GATAGAAGCCTTCGAGGCAGCACGCTGA

Upstream 100 bases:

>100_bases
TCTTAGCGGGGCCGCTTGTGGCCGCCGCCGCCGCTGTGACAGGTAAGATCACGGACCCGAGAGAACTGATGTAAAGACTA
ATCTAATAAAGTAGCATAAG

Downstream 100 bases:

>100_bases
TTATGATAGAAATAATGGATACAACCCTCAGAGATGGGGAGCAAACTTCGGGCGTCTCCTTCGCCGCTCACGAGAAGCTT
AGCATAGCGCAAGTGCTGCT

Product: 3-isopropylmalate dehydratase small subunit LeuD

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKTKFNIITSTCVPLPLENVDTDQIIPARFLKATTKEGFGENLFRDWRYDKEGNKIESFVLNDPTYSGKILVAGKNFGSG
SSREHAAWAIADYGFRVVVSSFFADIHKNNELNNFVLPVVVTEEFLAELFDSIYKDPKTEVEVNLPEQTITNKATGKSEH
FEINAYKKDCLINGLDDIDYLLANKSKIEAFEAAR

Sequences:

>Translated_195_residues
MKTKFNIITSTCVPLPLENVDTDQIIPARFLKATTKEGFGENLFRDWRYDKEGNKIESFVLNDPTYSGKILVAGKNFGSG
SSREHAAWAIADYGFRVVVSSFFADIHKNNELNNFVLPVVVTEEFLAELFDSIYKDPKTEVEVNLPEQTITNKATGKSEH
FEINAYKKDCLINGLDDIDYLLANKSKIEAFEAAR
>Mature_195_residues
MKTKFNIITSTCVPLPLENVDTDQIIPARFLKATTKEGFGENLFRDWRYDKEGNKIESFVLNDPTYSGKILVAGKNFGSG
SSREHAAWAIADYGFRVVVSSFFADIHKNNELNNFVLPVVVTEEFLAELFDSIYKDPKTEVEVNLPEQTITNKATGKSEH
FEINAYKKDCLINGLDDIDYLLANKSKIEAFEAAR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=45.4545454545455, Blast_Score=166, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=38.5786802030457, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22012; Mature: 22012

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKFNIITSTCVPLPLENVDTDQIIPARFLKATTKEGFGENLFRDWRYDKEGNKIESFV
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEE
LNDPTYSGKILVAGKNFGSGSSREHAAWAIADYGFRVVVSSFFADIHKNNELNNFVLPVV
ECCCCCCCEEEEEECCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEE
VTEEFLAELFDSIYKDPKTEVEVNLPEQTITNKATGKSEHFEINAYKKDCLINGLDDIDY
EHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHH
LLANKSKIEAFEAAR
HHHCCCHHHHHHCCC
>Mature Secondary Structure
MKTKFNIITSTCVPLPLENVDTDQIIPARFLKATTKEGFGENLFRDWRYDKEGNKIESFV
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEE
LNDPTYSGKILVAGKNFGSGSSREHAAWAIADYGFRVVVSSFFADIHKNNELNNFVLPVV
ECCCCCCCEEEEEECCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEE
VTEEFLAELFDSIYKDPKTEVEVNLPEQTITNKATGKSEHFEINAYKKDCLINGLDDIDY
EHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHH
LLANKSKIEAFEAAR
HHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA