Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 150008669

GI number: 150008669

Start: 2429703

End: 2430764

Strand: Reverse

Name: leuB [H]

Synonym: BDI_2059

Alternate gene names: 150008669

Gene position: 2430764-2429703 (Counterclockwise)

Preceding gene: 150008670

Following gene: 150008667

Centisome position: 50.52

GC content: 50.19

Gene sequence:

>1062_bases
ATGAAACTGAATATAGCAGTACTTCCCGGAGACGGGATCGGACCAGAGATTTCCGTGCAAGGCGTGGAAGTAATGAGTGC
CGTTTGTGAGAAATTCGGACATGAGGTTCACTATGAGTATGCCCTTTGTGGCGCTGACGCGATCGATAAGGTAGGCGATC
CTTTCCCGGAGGAGACCTATCGGATTTGTAAGGATGCCGACGCTGTTCTTTTCTCGGCCGTGGGCGATCCTAAGTTTGAT
AATGATCCGACAGCGAAAGTACGTCCGGAGCAAGGTCTTTTGGCGATGCGTAAGAAGTTGGGCCTGTTCGCTAATATCCG
TCCGGTGCAGACCTTCAAATGCCTATTGCATAAATCGCCGTTGCGTGCCGAATTGGTGGATGGCGCGGATTTCTTATGTA
TCCGTGAGTTGACCGGTGGTATGTATTTCGGTGAGAAATATCAAGACAACGATAAGGCATACGATACGAATATGTATACC
CGCCCGGAAATCGAGCGTATCTTGAAAGTGGGCTTCGAGTATGCGATGAAACGGAATAAGCACCTGACGGTTGTTGACAA
GGCGAACGTACTTGCCTCTTCCCGTCTATGGCGCCAGATCGCTCAAGAGATGGCTCCTAGCTATCCGGAAGTAACGACAG
ACTATATGTATGTGGACAACGCCGCTATGCGTATGATCCAAGAGCCGAAATTCTTCGACGTGATGGTAACCGAGAATACG
TTTGGTGATATCTTGACGGATGAGGGTTCTTGCATCAGCGGCTCTATGGGCTTGCTGCCTTCCGCTTCTACCGGAGAGAG
CACGCCGGTTTTCGAGCCGATTCATGGCTCTTGGCCGCAGGCGAAAGGGCTGAATATTGCGAACCCCTTGGCGCAAATTC
TTTCAGTAGCGATGTTATTCGAGTATTTCGACCTCAAAGAAGAGGGAGCCTTGATCCGTGAAGCCGTAGACGCTTCCCTC
GATGCGAATGTCCGTACGCCGGAAATCCAAGTGGAAGGTGGAGCGAAATATGGAACGAAAGAAGTGGGTAGTTGGGTTGT
CAATTATATCACGGAAAAGTAG

Upstream 100 bases:

>100_bases
CGACTTGATGATTACAAGTACCCTGTGGGATGATGACACGTATTTCCCCACGGAGCCTAAGTATGTACATTTTAACAGAG
AATAATAACAAACATTTACG

Downstream 100 bases:

>100_bases
TATAAATGGGCCGATTTCGCACCAGTAGTGGCGCAGGCTCACACGTATAGTGGCATCGGCTCACGCGAGTAGTGGCGTAG
GCCAATACCACTACTCGCGT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKLNIAVLPGDGIGPEISVQGVEVMSAVCEKFGHEVHYEYALCGADAIDKVGDPFPEETYRICKDADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLLHKSPLRAELVDGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVGFEYAMKRNKHLTVVDKANVLASSRLWRQIAQEMAPSYPEVTTDYMYVDNAAMRMIQEPKFFDVMVTENT
FGDILTDEGSCISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGALIREAVDASL
DANVRTPEIQVEGGAKYGTKEVGSWVVNYITEK

Sequences:

>Translated_353_residues
MKLNIAVLPGDGIGPEISVQGVEVMSAVCEKFGHEVHYEYALCGADAIDKVGDPFPEETYRICKDADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLLHKSPLRAELVDGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVGFEYAMKRNKHLTVVDKANVLASSRLWRQIAQEMAPSYPEVTTDYMYVDNAAMRMIQEPKFFDVMVTENT
FGDILTDEGSCISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGALIREAVDASL
DANVRTPEIQVEGGAKYGTKEVGSWVVNYITEK
>Mature_353_residues
MKLNIAVLPGDGIGPEISVQGVEVMSAVCEKFGHEVHYEYALCGADAIDKVGDPFPEETYRICKDADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLLHKSPLRAELVDGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVGFEYAMKRNKHLTVVDKANVLASSRLWRQIAQEMAPSYPEVTTDYMYVDNAAMRMIQEPKFFDVMVTENT
FGDILTDEGSCISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGALIREAVDASL
DANVRTPEIQVEGGAKYGTKEVGSWVVNYITEK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=323, Percent_Identity=30.9597523219814, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=27.3015873015873, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=27.3015873015873, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=27.3255813953488, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=27.8787878787879, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI28178819, Length=159, Percent_Identity=33.9622641509434, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=45.8100558659218, Blast_Score=307, Evalue=6e-85,
Organism=Escherichia coli, GI1788101, Length=319, Percent_Identity=34.1692789968652, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=21.1796246648794, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=29.1907514450867, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=30.0291545189504, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=27.0194986072423, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=331, Percent_Identity=29.0030211480363, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=40.8839779005525, Blast_Score=255, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=32.8690807799443, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=332, Percent_Identity=29.2168674698795, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=326, Percent_Identity=28.2208588957055, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24643270, Length=305, Percent_Identity=31.8032786885246, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643268, Length=305, Percent_Identity=31.8032786885246, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=337, Percent_Identity=28.7833827893175, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI281362242, Length=329, Percent_Identity=28.2674772036474, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=329, Percent_Identity=28.2674772036474, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.2988505747126, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=27.4566473988439, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130355, Length=205, Percent_Identity=24.390243902439, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39124; Mature: 39124

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNIAVLPGDGIGPEISVQGVEVMSAVCEKFGHEVHYEYALCGADAIDKVGDPFPEETY
CEEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCHHHH
RICKDADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLLHKSP
HHHCCCCHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVDGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYTRPEIERILKVGFEYAMKRNK
CHHHHCCCCCCEEHHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
HLTVVDKANVLASSRLWRQIAQEMAPSYPEVTTDYMYVDNAAMRMIQEPKFFDVMVTENT
EEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSCISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDLKEEGALIREAVDASLDANVRTPEIQVEGGAKYGTKEVGSWVVNYITEK
HHHCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNIAVLPGDGIGPEISVQGVEVMSAVCEKFGHEVHYEYALCGADAIDKVGDPFPEETY
CEEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCHHHH
RICKDADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLLHKSP
HHHCCCCHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVDGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYTRPEIERILKVGFEYAMKRNK
CHHHHCCCCCCEEHHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
HLTVVDKANVLASSRLWRQIAQEMAPSYPEVTTDYMYVDNAAMRMIQEPKFFDVMVTENT
EEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSCISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDLKEEGALIREAVDASLDANVRTPEIQVEGGAKYGTKEVGSWVVNYITEK
HHHCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA