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The map label for this gene is vsr [H]

Identifier: 150008636

GI number: 150008636

Start: 2390041

End: 2390574

Strand: Reverse

Name: vsr [H]

Synonym: BDI_2024

Alternate gene names: 150008636

Gene position: 2390574-2390041 (Counterclockwise)

Preceding gene: 150008637

Following gene: 150008627

Centisome position: 49.69

GC content: 46.25

Gene sequence:

>534_bases
ATGACAGATCTTATCTCTAAAGAAAAGCGTAGCTATATCATGTCTATGATTAAGGGCAAGAATACAAAGCCCGAGATTAT
AGTCCGTAAATACCTGTTCCATTGTGGCTTTCGCTTTCGTGTGAATGTAAAGCGCCTTCCCGGTACCCCTGATATCGTAT
TGCGTAAATATAAGACCGCTATCTTCGTGAACGGCTGTTTCTGGCATGGGCACGAGGGTTGCCCTGAATTTCGTCCTCCC
CGTACCCGGGTAGAGTGGTGGACCGAGAAACTAAACCGGAATAAAGCCCGGGATGCCCGTGTACGTGAGGAATTACGAGC
GATGGGCTGGAATACGATGGTGATTTGGGAATGCCAGCTTAAACCCAAAGAACGGCAAGCTACTTTAGAAAATGTCGTCC
GTTTATTGGAAAAGACGTATGTGGAATCTACCTATCATGCGAAGTTTACCCCAGCCTACCCGCTATTAGAGGAGGAAGAA
CTTCCCAAAGTGGCGGAAGAAGAGATATCCTATGAGAGTGCTCATCATTATTAA

Upstream 100 bases:

>100_bases
AGGACCTTCCTATTTGATTACGAAGATTGATCCGGCCAAGAGTATCCGTTTTGAATAAAAGCCGTATCTTTGGCATACGT
TCTAGTTTTGCCGGATTGAT

Downstream 100 bases:

>100_bases
TTCTCCAACCGGAACGTATAATCCTTCCTACTTCTGAAAAGCCGGTAAATACGGACATATCCATTGAATATGGGTTGAAT
AAACTCCAACAGGAATAACC

Product: putative very-short-patch-repair endonuclease

Products: DNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTDLISKEKRSYIMSMIKGKNTKPEIIVRKYLFHCGFRFRVNVKRLPGTPDIVLRKYKTAIFVNGCFWHGHEGCPEFRPP
RTRVEWWTEKLNRNKARDARVREELRAMGWNTMVIWECQLKPKERQATLENVVRLLEKTYVESTYHAKFTPAYPLLEEEE
LPKVAEEEISYESAHHY

Sequences:

>Translated_177_residues
MTDLISKEKRSYIMSMIKGKNTKPEIIVRKYLFHCGFRFRVNVKRLPGTPDIVLRKYKTAIFVNGCFWHGHEGCPEFRPP
RTRVEWWTEKLNRNKARDARVREELRAMGWNTMVIWECQLKPKERQATLENVVRLLEKTYVESTYHAKFTPAYPLLEEEE
LPKVAEEEISYESAHHY
>Mature_176_residues
TDLISKEKRSYIMSMIKGKNTKPEIIVRKYLFHCGFRFRVNVKRLPGTPDIVLRKYKTAIFVNGCFWHGHEGCPEFRPPR
TRVEWWTEKLNRNKARDARVREELRAMGWNTMVIWECQLKPKERQATLENVVRLLEKTYVESTYHAKFTPAYPLLEEEEL
PKVAEEEISYESAHHY

Specific function: May nick specific sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=125, Percent_Identity=40, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 21162; Mature: 21031

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLISKEKRSYIMSMIKGKNTKPEIIVRKYLFHCGFRFRVNVKRLPGTPDIVLRKYKTA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHE
IFVNGCFWHGHEGCPEFRPPRTRVEWWTEKLNRNKARDARVREELRAMGWNTMVIWECQL
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECC
KPKERQATLENVVRLLEKTYVESTYHAKFTPAYPLLEEEELPKVAEEEISYESAHHY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHCHHHCCCC
>Mature Secondary Structure 
TDLISKEKRSYIMSMIKGKNTKPEIIVRKYLFHCGFRFRVNVKRLPGTPDIVLRKYKTA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHE
IFVNGCFWHGHEGCPEFRPPRTRVEWWTEKLNRNKARDARVREELRAMGWNTMVIWECQL
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECC
KPKERQATLENVVRLLEKTYVESTYHAKFTPAYPLLEEEELPKVAEEEISYESAHHY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]