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The map label for this gene is hisF

Identifier: 150008630

GI number: 150008630

Start: 2384106

End: 2384861

Strand: Direct

Name: hisF

Synonym: BDI_2018

Alternate gene names: 150008630

Gene position: 2384106-2384861 (Clockwise)

Preceding gene: 150008629

Following gene: 150008631

Centisome position: 49.55

GC content: 52.38

Gene sequence:

>756_bases
ATGCTAGCCAAGAGAATTGTTCCCTGTCTGGATATAAAGGATGGAAAGACCGTAAAAGGCATCAACTTCGTGAACTTTCG
CGACGCTGGAGACCCCGTGGAGTTGGGCGCCCAGTACAGCCGTGAAGGGGCCGACGAATTAGTGTACTTGGATATTACCG
CCTCACACGAGGGGCGAAAGACTTTCACCGAGTTGGTAAAACAAGTGGCGGCCCATATCAGCATCCCTTTTACCGTGGGT
GGAGGTATCAACGAATTGAAGGACGTGGATCGTTTGCTTAGCGCTGGCGCCGATAAGGTCTCGATCAACTCGGCGGCTTT
ACGTAACCCGGCATTAATCGAGGAGATCGCCAAGAACTTTGGTAGTCAGGTATGCGTAGTCGCTATCGACGCTAATTTTG
AGAACAATGACTGGCTATGCTATCTAAACGGAGGGCGTGTCCCGACGGATAAGTATTTGTTTCAATGGGCCTCGGAAGCG
GAAAGCCGTGGCGCCGGCGAGATCCTTTTCACCAGTATGACGCACGATGGCGTAAAAGACGGATACGCGAACGACGCTCT
GGCTACCTTGGCCGATACGTTGCATATCCCGGTTATCGCCTCCGGTGGAGCGGGAAAGATGGAACATTTCCGGGATACGT
TTTCCAACGGGAAAGCGGGCGCGGCTTTAGCGGCCAGCGTTTTCCATTTCGGCGAGATACGGATTAGCGACTTGAAGCAA
TACCTCAAGGACGAGGGGATCAATGTAAGGATATAA

Upstream 100 bases:

>100_bases
AGGCTGGGATTCCCGCTGTCATTTTCGGCAAGGCCATTTATGAGGGGAAGATCCAGTTGAAAGATTTACTTCGTTTTACT
TGAAATAATAAAATAGATAT

Downstream 100 bases:

>100_bases
CACATTATATATAAAGATGAAATTAGATTTCGAGAAAATGGGCGGATTAATACCCGCTATCGTACAAGACAACAACACGA
GTAAGGTATTAATGTTGGGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIVPCLDIKDGKTVKGINFVNFRDAGDPVELGAQYSREGADELVYLDITASHEGRKTFTELVKQVAAHISIPFTVG
GGINELKDVDRLLSAGADKVSINSAALRNPALIEEIAKNFGSQVCVVAIDANFENNDWLCYLNGGRVPTDKYLFQWASEA
ESRGAGEILFTSMTHDGVKDGYANDALATLADTLHIPVIASGGAGKMEHFRDTFSNGKAGAALAASVFHFGEIRISDLKQ
YLKDEGINVRI

Sequences:

>Translated_251_residues
MLAKRIVPCLDIKDGKTVKGINFVNFRDAGDPVELGAQYSREGADELVYLDITASHEGRKTFTELVKQVAAHISIPFTVG
GGINELKDVDRLLSAGADKVSINSAALRNPALIEEIAKNFGSQVCVVAIDANFENNDWLCYLNGGRVPTDKYLFQWASEA
ESRGAGEILFTSMTHDGVKDGYANDALATLADTLHIPVIASGGAGKMEHFRDTFSNGKAGAALAASVFHFGEIRISDLKQ
YLKDEGINVRI
>Mature_251_residues
MLAKRIVPCLDIKDGKTVKGINFVNFRDAGDPVELGAQYSREGADELVYLDITASHEGRKTFTELVKQVAAHISIPFTVG
GGINELKDVDRLLSAGADKVSINSAALRNPALIEEIAKNFGSQVCVVAIDANFENNDWLCYLNGGRVPTDKYLFQWASEA
ESRGAGEILFTSMTHDGVKDGYANDALATLADTLHIPVIASGGAGKMEHFRDTFSNGKAGAALAASVFHFGEIRISDLKQ
YLKDEGINVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=46.692607003891, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI87082028, Length=180, Percent_Identity=28.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=31.8471337579618, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PARD8 (A6LDI7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303373.1
- ProteinModelPortal:   A6LDI7
- SMR:   A6LDI7
- STRING:   A6LDI7
- GeneID:   5307168
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2018
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   PDIS435591:BDI_2018-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27092; Mature: 27092

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDIKDGKTVKGINFVNFRDAGDPVELGAQYSREGADELVYLDITASHEGRK
CCHHHCCCCEECCCCCEECCEEEEEECCCCCHHHHCCHHCCCCCCCEEEEEEECCCCCHH
TFTELVKQVAAHISIPFTVGGGINELKDVDRLLSAGADKVSINSAALRNPALIEEIAKNF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQVCVVAIDANFENNDWLCYLNGGRVPTDKYLFQWASEAESRGAGEILFTSMTHDGVKD
CCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
GYANDALATLADTLHIPVIASGGAGKMEHFRDTFSNGKAGAALAASVFHFGEIRISDLKQ
CCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEHHHHHH
YLKDEGINVRI
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIVPCLDIKDGKTVKGINFVNFRDAGDPVELGAQYSREGADELVYLDITASHEGRK
CCHHHCCCCEECCCCCEECCEEEEEECCCCCHHHHCCHHCCCCCCCEEEEEEECCCCCHH
TFTELVKQVAAHISIPFTVGGGINELKDVDRLLSAGADKVSINSAALRNPALIEEIAKNF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQVCVVAIDANFENNDWLCYLNGGRVPTDKYLFQWASEAESRGAGEILFTSMTHDGVKD
CCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
GYANDALATLADTLHIPVIASGGAGKMEHFRDTFSNGKAGAALAASVFHFGEIRISDLKQ
CCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEHHHHHH
YLKDEGINVRI
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA