Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 150008629

GI number: 150008629

Start: 2383369

End: 2384088

Strand: Direct

Name: hisA

Synonym: BDI_2017

Alternate gene names: 150008629

Gene position: 2383369-2384088 (Clockwise)

Preceding gene: 150008628

Following gene: 150008630

Centisome position: 49.54

GC content: 49.03

Gene sequence:

>720_bases
ATGATTGAGCTTATTCCCGCTATAGATATAATAGATGGCAAATGCGTCCGGTTGACGCAAGGAGATTACGCGACCAAGAA
GGTTTACAACGAAGATCCTCTGGAGGTGGCCAAGATGTTCGAGGGTAACGGCATCCGGCGTTTGCATGTGGTAGATTTGG
ATGGAGCCCGTGAAGGACGCATCATCAACTACCGTATCCTTGAGCGGATAGCGACCCGGACTTCCTTGATCATTGATTTC
GGAGGGGGCTTGAAACAAGAGGATGATCTGGAGATCGCCTTCGAAAGCGGCGCCCAGATGGTAACCGGAGGCAGTATCGC
CGTGAAGAACCCGGAAATGTTCACCTCTTGGATCAGCAAGTTCGGTAGCGAGAAAATCATATTAGGGGCCGATGCCAAAG
AAAAGAAGATCGCCATCAGCGGTTGGGAGGAAACGACCAGCCAAGAGTTGGTTCCTTTTATCAAAGGATATTACGACAAG
GGGATTACCAAGGTGATCTGCACGGATATCGCACGGGATGGTATGCTGCAAGGCCCGGCTATCGATTTATATAAAGAGAT
ACGGGACGAGATCCCCTTCCTTTATATCATAGCGAGCGGTGGCGTAAGCTCGATCGAGGATATTGAGAAATTATCTGAGG
CTGGGATTCCCGCTGTCATTTTCGGCAAGGCCATTTATGAGGGGAAGATCCAGTTGAAAGATTTACTTCGTTTTACTTGA

Upstream 100 bases:

>100_bases
TCAGCGCCGCTTTACATAAGGACAACTTCTACGCGACACAGTTCCATCCGGAAAAAAGTGGATCGGTAGGAGAGGTTATA
TTGAAAAACTTTTTAAAGAT

Downstream 100 bases:

>100_bases
AATAATAAAATAGATATATGCTAGCCAAGAGAATTGTTCCCTGTCTGGATATAAAGGATGGAAAGACCGTAAAAGGCATC
AACTTCGTGAACTTTCGCGA

Product: imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIELIPAIDIIDGKCVRLTQGDYATKKVYNEDPLEVAKMFEGNGIRRLHVVDLDGAREGRIINYRILERIATRTSLIIDF
GGGLKQEDDLEIAFESGAQMVTGGSIAVKNPEMFTSWISKFGSEKIILGADAKEKKIAISGWEETTSQELVPFIKGYYDK
GITKVICTDIARDGMLQGPAIDLYKEIRDEIPFLYIIASGGVSSIEDIEKLSEAGIPAVIFGKAIYEGKIQLKDLLRFT

Sequences:

>Translated_239_residues
MIELIPAIDIIDGKCVRLTQGDYATKKVYNEDPLEVAKMFEGNGIRRLHVVDLDGAREGRIINYRILERIATRTSLIIDF
GGGLKQEDDLEIAFESGAQMVTGGSIAVKNPEMFTSWISKFGSEKIILGADAKEKKIAISGWEETTSQELVPFIKGYYDK
GITKVICTDIARDGMLQGPAIDLYKEIRDEIPFLYIIASGGVSSIEDIEKLSEAGIPAVIFGKAIYEGKIQLKDLLRFT
>Mature_239_residues
MIELIPAIDIIDGKCVRLTQGDYATKKVYNEDPLEVAKMFEGNGIRRLHVVDLDGAREGRIINYRILERIATRTSLIIDF
GGGLKQEDDLEIAFESGAQMVTGGSIAVKNPEMFTSWISKFGSEKIILGADAKEKKIAISGWEETTSQELVPFIKGYYDK
GITKVICTDIARDGMLQGPAIDLYKEIRDEIPFLYIIASGGVSSIEDIEKLSEAGIPAVIFGKAIYEGKIQLKDLLRFT

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=236, Percent_Identity=35.1694915254237, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=21.8106995884774, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PARD8 (A6LDI6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303372.1
- ProteinModelPortal:   A6LDI6
- STRING:   A6LDI6
- GeneID:   5307167
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2017
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   CLSK822682
- BioCyc:   PDIS435591:BDI_2017-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26456; Mature: 26456

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELIPAIDIIDGKCVRLTQGDYATKKVYNEDPLEVAKMFEGNGIRRLHVVDLDGAREGR
CEEEECEEEECCCCEEEEECCCCHHHHCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCC
IINYRILERIATRTSLIIDFGGGLKQEDDLEIAFESGAQMVTGGSIAVKNPEMFTSWISK
EEHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCEEEECCEEEEECHHHHHHHHHH
FGSEKIILGADAKEKKIAISGWEETTSQELVPFIKGYYDKGITKVICTDIARDGMLQGPA
HCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCH
IDLYKEIRDEIPFLYIIASGGVSSIEDIEKLSEAGIPAVIFGKAIYEGKIQLKDLLRFT
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIELIPAIDIIDGKCVRLTQGDYATKKVYNEDPLEVAKMFEGNGIRRLHVVDLDGAREGR
CEEEECEEEECCCCEEEEECCCCHHHHCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCC
IINYRILERIATRTSLIIDFGGGLKQEDDLEIAFESGAQMVTGGSIAVKNPEMFTSWISK
EEHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCEEEECCEEEEECHHHHHHHHHH
FGSEKIILGADAKEKKIAISGWEETTSQELVPFIKGYYDKGITKVICTDIARDGMLQGPA
HCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCH
IDLYKEIRDEIPFLYIIASGGVSSIEDIEKLSEAGIPAVIFGKAIYEGKIQLKDLLRFT
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA