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The map label for this gene is hisH [H]

Identifier: 150008628

GI number: 150008628

Start: 2382782

End: 2383372

Strand: Direct

Name: hisH [H]

Synonym: BDI_2016

Alternate gene names: 150008628

Gene position: 2382782-2383372 (Clockwise)

Preceding gene: 150008626

Following gene: 150008629

Centisome position: 49.52

GC content: 43.82

Gene sequence:

>591_bases
ATGGACGTTGCCATTATTAAATATAATGCCGGAAACATCTACTCGGTAGATTACGCTTTGAAGCGGTTGGGCGTTACCCC
GATCATCACGGCTGATCCGGAATTATTAAATAAAGCGGATAAGGTTATCTTTCCCGGAGTAGGTGAAGCTTTCACGACCA
TGGAGTATTTAAGGGAGCATAAATTGGATCAGGTGATCTTGAACCTAAAGCAACCGGTACTCGGGATATGCCTCGGCATG
CAATTGATGTGCATGCGTTCAGAGGAGGGAAACGCGGATTGCTTGGGGATCTTCCCTACGGAAGTGAAACGTTTCATTCC
GCGAAAGCATGAGGACAAGGTTCCTCACATGGGTTGGAATACTTTGACAAACGTTAAAAGCGGCCTATTCAATGAGCGAT
TGGAGAACAAATTTGTTTACTTCGTACATAGTTATTATGTACCTGTAAATGAATATACGGCGGCTACGACCGATTACATC
CTTCCGTTCAGCGCCGCTTTACATAAGGACAACTTCTACGCGACACAGTTCCATCCGGAAAAAAGTGGATCGGTAGGAGA
GGTTATATTGAAAAACTTTTTAAAGATATGA

Upstream 100 bases:

>100_bases
TATCACAAATACATTTCATTTTGGAACTTTTCAATCGCCATTCATAATTATGTAATGATAATTTGTTACTTTTGCGACTC
AATATAAATGCTTAACAACA

Downstream 100 bases:

>100_bases
TTGAGCTTATTCCCGCTATAGATATAATAGATGGCAAATGCGTCCGGTTGACGCAAGGAGATTACGCGACCAAGAAGGTT
TACAACGAAGATCCTCTGGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MDVAIIKYNAGNIYSVDYALKRLGVTPIITADPELLNKADKVIFPGVGEAFTTMEYLREHKLDQVILNLKQPVLGICLGM
QLMCMRSEEGNADCLGIFPTEVKRFIPRKHEDKVPHMGWNTLTNVKSGLFNERLENKFVYFVHSYYVPVNEYTAATTDYI
LPFSAALHKDNFYATQFHPEKSGSVGEVILKNFLKI

Sequences:

>Translated_196_residues
MDVAIIKYNAGNIYSVDYALKRLGVTPIITADPELLNKADKVIFPGVGEAFTTMEYLREHKLDQVILNLKQPVLGICLGM
QLMCMRSEEGNADCLGIFPTEVKRFIPRKHEDKVPHMGWNTLTNVKSGLFNERLENKFVYFVHSYYVPVNEYTAATTDYI
LPFSAALHKDNFYATQFHPEKSGSVGEVILKNFLKI
>Mature_196_residues
MDVAIIKYNAGNIYSVDYALKRLGVTPIITADPELLNKADKVIFPGVGEAFTTMEYLREHKLDQVILNLKQPVLGICLGM
QLMCMRSEEGNADCLGIFPTEVKRFIPRKHEDKVPHMGWNTLTNVKSGLFNERLENKFVYFVHSYYVPVNEYTAATTDYI
LPFSAALHKDNFYATQFHPEKSGSVGEVILKNFLKI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=44.9494949494949, Blast_Score=169, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22268; Mature: 22268

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAIIKYNAGNIYSVDYALKRLGVTPIITADPELLNKADKVIFPGVGEAFTTMEYLREH
CCEEEEEECCCCEEEHHHHHHHCCCCEEEECCHHHHCCCCCEEECCCCHHHHHHHHHHHH
KLDQVILNLKQPVLGICLGMQLMCMRSEEGNADCLGIFPTEVKRFIPRKHEDKVPHMGWN
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCH
TLTNVKSGLFNERLENKFVYFVHSYYVPVNEYTAATTDYILPFSAALHKDNFYATQFHPE
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCHHCCHHHHHCCCCEEEEEECCC
KSGSVGEVILKNFLKI
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDVAIIKYNAGNIYSVDYALKRLGVTPIITADPELLNKADKVIFPGVGEAFTTMEYLREH
CCEEEEEECCCCEEEHHHHHHHCCCCEEEECCHHHHCCCCCEEECCCCHHHHHHHHHHHH
KLDQVILNLKQPVLGICLGMQLMCMRSEEGNADCLGIFPTEVKRFIPRKHEDKVPHMGWN
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCH
TLTNVKSGLFNERLENKFVYFVHSYYVPVNEYTAATTDYILPFSAALHKDNFYATQFHPE
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCHHCCHHHHHCCCCEEEEEECCC
KSGSVGEVILKNFLKI
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA